Synopsis
Hamster mast cells have been found to give strong peroxidatic reactions at pH 5, 7.5 and 10 when sections of skeletal muscle are incubated for 2.5 h in the dark at room temperature on semipermeable membranes covering a gelled incubation medium consisting of 0.01% hydrogen peroxide, 5.5 mM diaminobenzidine and 1.36% agar dissolved in Universal buffer. The techniques is very efficient: with it, all mast cells react in marked contrast to the negative reaction they usually give with conventional techniques.
The peroxidatic reactions are abolished if tissues are perfused beforehand with either aminotriazole or KCN but not if these inhibitors are incorporated in the gelled incubation medium. This and other evidence suggests that the mast cell reactions are not due to either catalase or haemoglobin adsorbed onto mast cell granules from lysed red blood cells.
Skeletal muscle fibres do not exhibit any visible-peroxidase activity with the membrane technique.
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Christie, K. N. &Stoward, P. J. (1977a) Endogenous peroxidase in muscle fibres.Proc. Roy. micr. Soc. 12, 120–1.
Christie, K. N. & Stoward, P. J. (1977b). Cytochemical localisation of endogenous peroxidase in the sarcoplasmic reticulum of hamster skeletal muscle.Riv. Istochim. Norm. Patol. (in press).
Desaga, J. F. (1972). Das Nachweiss von Myeloperoxydase in jungen Gewebsmaztzellen der Ratte.Klin. Wschr. 50, 444–5.
Dvorak, A. M., Dvorak, H. F. &Karnovsky, M. J. (1972). Cytochemical localisation of peroxidase activity in the developing erythrocyte.Am. J. Path. 67, 303–15.
Fahimi, H. D. (1970). The fine structural localisation of endogenous and exogenous peroxidase activity in Kupffer cells of rat liver.J. Cell Biol. 47, 247–62.
Fahimi, H. D. (1973). Diffusion artifacts in cytochemistry of catalase.J. Histochem. Cytochem. 21, 999–1009.
Hand, A. R. (1974). Peroxisomes (microbodies) in striated muscle cells.J. Histochem. Cytochem. 22, 207–9.
Herzog, V. &Fahimi, H. D. (1973). A new colorimetric assay for peroxidase using 3,3′-diaminobenzidine as hydrogen donor.Analyt. Biochem. 55, 554–62.
Herzog, V. &Fahimi, H. D. (1976). Intracellular distinction between peroxidase and catalase in exocrine cells of rat lacrimal gland: a biochemical and cytochemical study.Histochemistry 46, 273–86.
Lojda, Z., Gossrau, R. &Schiebler, T. H. (1976).Enzym-histochemische Methoden. Berlin, Heidelberg & New York: Springer-Verlag.
Meijer, A. E. F. H. (1972). Semipermeable membranes for improving the histochemical demonstration of enzyme activities in tissue sections. 1. Acid phosphatase.Histochemie 30, 31–9.
Meijer, A. E. F. H. & deVries, G. P. (1974). Semipermeable membranes for improving the histochemical demonstration of enzyme activities in tissue sections. IV. Glucose 6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase (decarboxylating).Histochemistry 40, 349–59.
Novikoff, A. B., Beard, M. E., Albala, A., Sheid, B., Quintana, N. &Biempica, L. (1971). Localisation of endogenous peroxidases in animal tissues.J. Microscopie 12, 381–404.
Okun, M. R., Hamada, G., Donnellan, B. &Lever, W. F. (1971). Latency of the Dopa reaction in mast cells.Histochemie 25, 1–8.
Okun, M. R., Edelstein, L. M., Patel, R. P. &Donnellan, B. (1973). A revised concept of mammalian melanogenesis: the possible synergistic functions of aerobic dopa oxidase and peroxidase. A review.Yale J. Biol. Med. 46, 535–40.
Rechcigl, M. &Evans, W. H. (1963). Role of catalase and peroxidase in the metabolism of leucocytes.Nature 199, 1001–2.
Robbins, D., Fahimi, H. D. &Cotran, R. S. (1971). Fine structural cytochemical localisation of peroxidase activity in rat peritoneal cells: mononuclear cells, eosinophils and mast cells.J. Histochem. Cytochem. 19, 571–5.
Selye, H. (1965).The Mast Cells, p. 50. Washington: Butterworths.
Somer, J. R. &Waugh, R. A. (1976). The ultrastructure of the mammalian cardiac inuscle cell—with special emphasis on the tubular membrane systems.Am. J. Path. 82, 191–221.
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Christie, K.N., Stoward, P.J. Endogenous peroxidase in mast cells localized with a semipermeable membrane technique. Histochem J 10, 425–433 (1978). https://doi.org/10.1007/BF01003006
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DOI: https://doi.org/10.1007/BF01003006