The aim of the present work was to develop a method for the simultaneous detection of mast cells (MC) and nerve fiber terminals based on methods developed for the histochemical identification of MC with Alcian blue and an immunochemical reaction for synaptophysin. The protocol developed here provides highly selective and reproducible simultaneous detection of MC and nerve endings on paraffin sections of thymus from laboratory mammals. The method can be used both for detecting the spatial interactions of MC and nerve terminals and for independent studies of the innervation of the internal organs of mammals. The optimum fixative was zinc-ethanol-formaldehyde.
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Translated from Morfologiya, Vol. 145, No. 2, pp. 70–73, March–April, 2014.
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Gusel’nikova, V.V., Sukhorukova, E.G., Fedorova, E.A. et al. A Method for the Simultaneous Detection of Mast Cells and Nerve Terminals in the Thymus in Laboratory Mammals. Neurosci Behav Physi 45, 371–374 (2015). https://doi.org/10.1007/s11055-015-0084-x
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DOI: https://doi.org/10.1007/s11055-015-0084-x