Abstract
By means of a bioassay employing the minnowPimephales promelas, the anesthetic activities and toxicities of various defensive steroids and norsesquiterpenes produced by the Dytiscidae and Gyrinidae were compared with those of a wide selection of steroid standards. The most widely occurring major components of dytiscid secretions, 4-pregnen-3-ones and related derivatives, were among those compounds most active in minnow bioassays. The norsesquiterpenes gyrinidal and gyrinidione were among the most toxic compounds tested but they possessed little anesthetic action. The anesthetic activity of gyrinidone was comparable to that of testosterone. Steroid activity in the minnow bioassay was highly related to the degree of oxygenation; steroids oxygenated only at the termini of the molecule were most active. Less or additional oxygenation resulted in a loss of activity. When steroids were rapidly administered to minnows the activities of many of them were similar, suggesting they share a common mode of action.
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Miller, J.R., Mumma, R.O. Physiological activity of water beetle defensive agents. I. Toxicity and anesthetic activity of steroids and norsesquiterpenes administered in solution to the minnowPimephales promelas Raf.. J Chem Ecol 2, 115–130 (1976). https://doi.org/10.1007/BF00987736
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DOI: https://doi.org/10.1007/BF00987736