Abstract
Phosphate uptake was studied by determining [32P]phosphate influx and by measurements of the electrical membrane potential in duckweed (Lemna gibba L.). Phosphate-induced membrane depolarization (ΔE m ) was controlled by the intracellular phosphate content, thus maximal ΔE m by 1 mM H2PO -4 was up to 133 mV after 15d of phosphate starvation. The ΔE m was strongly dependent on the extracellular pH, with a sharp optimum at pH 5.7. It is suggested that phosphate uptake is energized by the electrochemical proton gradient, proceeding by a 2H+/H2PO -4 contransport mechanism. This is supported also by the fusicoccin stimulation of phosphate influx. Kinetics of phosphate influx and of ΔE m , which represent mere plasmalemma transport, are best described by two Michaelis-Menten terms without any linear components.
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Abbreviations
- E m :
-
electrical membrane potential difference
- ΔE m :
-
phosphate-induced, maximal membrane depolarization
- FW:
-
fresh weight
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Ullrich-Eberius, C.I., Novacky, A. & van Bel, A.J.E. Phosphate uptake inLemna gibba G1: energetics and kinetics. Planta 161, 46–52 (1984). https://doi.org/10.1007/BF00951459
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DOI: https://doi.org/10.1007/BF00951459