Abstract
The localization of catecholamines was documented in the cercaria ofSchistosoma mansoni andS. japonicum by a fluorescent histochmical method using glyoxylic acid (GA). Cell bodies and nerve fibres were spatially visualized in wholemount preparations, and the fluorescent traces were investigated. The nervous system was bilaterally symmetrical, showing a similar formation in both species. A pair of cerebral ganglia with a transverse commussure showed a complex network of fluorophores, and each radiated two sets of anterior and posterior longitudinal nerve cords. A third pair of longitudinal nerve cords was observed in the most dorsal area. A posterior transverse commissure was seen to connect the posterior longitudinal cords, and the posterior terminals of the postero-ventral cords communicated with the tail cords. The glyoxylic acid-induced fluorescence (GAIF) method was demonstrated to be quite suitable for neuroanatomical and neurophysiological investigations of larval forms.
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Orido, Y. Histochemical evidence of the catecholamine-associated nervous system in certain schistosome cercariae. Parasitol Res 76, 146–149 (1989). https://doi.org/10.1007/BF00930837
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DOI: https://doi.org/10.1007/BF00930837