Summary
A phosphate-limited resting cell system ofStreptomyces griseus in a synthetic medium has been developed in which biosynthesis of the polyene macrolide, candicidin, is linear for at least 36 h without cell growth. Glucose and to a lesser degree sucrose, but not lactose, support antibiotic synthesis. Glucose is utilized at a constant rate for antibiotic synthesis without affecting mycelial dry weight. Acetate and propionate, the building units of the macrolide aglycone, stimulate candicidin biosynthesis in cultures supplemented with glucose but do not support its synthesis in the absence of glucose. Maximal stimulation of candicidin biosynthesis was produced by 40 mM propionate or 250 mM acetate. The biosynthetic intermediate, methyl malonate, and the analog, 1-propanol, were more stimulatory than propionate at the same concentration.
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References
Corcoran,J.W., Chick,M. (1966). Biochemistry of the macrolide antibiotics. In: Biosynthesis of antibiotics, J.F.Snell, ed., pp.159–201. New York: Academic Press
Day,L.E., Chamberlin,J.W., Gordee,E.Z., Chen,S., Gorman,M., Hamill,R.L., Ness,T., Weeks,R.E., Stroshane,R. (1973). Antimicrob. A. Chemother.4, 410–414
Demain,A.L. (1972). J. Appl. Chem. Biotechnol.22, 345–362
Liu,C.M., McDaniel,L.E., Schaffner,C.P. (1972). J. Antibiot.25, 116–121
Liu,C.M., McDaniel,L.E., Schaffner,C.P. (1975). Antimicrob. Ag. Chemother.7, 196–202
Magasanik,B. (1961). Cold Spring Harbor Symposium Quantitative Biology26, 249–256
Majumdar,M.K., Majumdar,S.K. (1971). Folia Microbiol.16, 285–292
Martin,J.F., McDaniel,L.E. (1974). Dev. Ind. Microbiol.15, 324–337
Martin,J.F., McDaniel,L.E. (1975a). J. Chromatogr.104, 151–160
Martin,J.F., McDaniel,L.E. (1975b). Biotech. Bioengineer.17, 925–938
Martin,J.F., McDaniel,L.E. (1975c). Biochim. Biophys. Acta411, 186–194
Omura,S., Nakagawa, A. (1975). J. Antibiot.28, 401–433
Raczynska-Bojanowska,K., Ruczaj,Z., Ostrowska-Krysiak,B., Roszkowski,J., Gaworowska-Michalik,J., Sawnor-Korszynska,D. (1970). Acta Microbiol. Pol. B2, 103–110
Rafalski,A., Raczynska-Bojanowska,K. (1972). Acta Biochim. Pol.19, 71–87
Volpe,J.J., Vagelos,P.P. (1973). Ann. Rev. Biochem.42, 21–60
Waksman,S.A., Lechevalier,H.A., Schaffner,C.P. (1965). Bull. Wld. Hlth. Org.33, 219–226
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Martin, J.F., McDaniel, L.E. Biosynthesis of candicidin by phosphate-limited resting cells ofStreptomyces griseus . European J. Appl Microbiol. 3, 135–144 (1976). https://doi.org/10.1007/BF00928432
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DOI: https://doi.org/10.1007/BF00928432