Abstract
The effects of pH, aeration, and temperature on the growth of fungal strain Mortierella alpina LPM-301 and the synthesis of lipids and arachidonic acid in glycerol-containing medium were studied. Arachidonic acid production in the stationary growth phase was found to depend considerably on the pH value; it reached the optimum at pH 6.0 and was irreversibly inhibited at a pH of 3.0. The pO2 values in a range from 10 to 50% showed no marked effect on mycelium growth or the synthesis of lipids and arachidonic acid. The temperature optimum for arachidonic acid production was 20–22°C. Under continuous cultivation, the amount of arachidonic acid reached 29.8% of lipids and 7.4% of biomass. The arachidonic acid yield from the glycerol consumed was 4.1% by mass and 8.8% by energy. It is suggested that arachidonic acid synthesis at an unfavorable pH and elevated temperatures was limited by the activity of Δ-12-desaturase and by the conversion of linoleic to arachidonic acid, respectively.
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Original Russian Text © E.G. Dedyukhina, T.I. Chistyakova, A.A. Mironov, S.V. Kamzolova, I.G. Minkevich, M.B. Vainshtein, 2015, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2015, Vol. 51, No. 2, pp. 243–250.
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Dedyukhina, E.G., Chistyakova, T.I., Mironov, A.A. et al. The effect of pH, aeration, and temperature on arachidonic acid synthesis by Mortierella alpina . Appl Biochem Microbiol 51, 242–248 (2015). https://doi.org/10.1134/S0003683815020040
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DOI: https://doi.org/10.1134/S0003683815020040