Abstract
Highly synchronous cultures ofPlasmodium falciparum were exposed to therapeutic concentrations of sulfadoxine or pyrimethamine at different developmental stages to investigate the effect on subsequent growth. Morphological observations showed that schizont formation from uninuclear trophozoites was the only process inhibited by the drugs. Segmentation of mature schizonts, merozoite invasion and development of the ring stage remained unaffected. These results support earlier reports suggesting that DNA synthesis is most pronounced in 32–42 h old trophozoites. The possible relevance of our results to the metabolism ofP. falciparum is discussed.
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Assaraf YG, Golenser J, Spira DT, Bachrach U (1984) Polyamine levels and the activity of their biosynthetic enzymes in human erythrocytes infected with the malarial parasite,Plasmodium falciparum. Biochem J 222:815–819
Dieckmann A, Jung A (1986a) Mechanisms of sulfadoxine resistance inPlasmodium falciparum. Mol Biochem Parasitol 19:143–147
Dieckmann A, Jung A (1986b) The mechanism of pyrimethamine resistance inPlasmodium falciparum. Parasitology (in press)
Gritzmacher CA, Reese RT (1984) Protein and nucleic acid synthesis during synchronized growth ofPlasmodium falciparum. J Bact 160:1165–1167
Inselburg J, Banyal HS (1984a) Synthesis of DNA during the asexual cycle ofPlasmodium falciparum in culture. Mol Biochem Parasitol 10:79–87
Inselburg J, Banyal HS (1984b)Plasmodium falciparum: Synchronization of asexual development with aphidicolin, a DNA synthesis inhibitor. Exp Parasitol 57:48–54
Lambros C, Vanderberg JP (1979) Synchronization ofPlasmodium falciparum erythrocytic stages in culture. J Parasitol 65:418–420
Trager W, Jensen JB (1976) Human malaria parasites in continuous culture. Science 193:763–765
Vial HJ, Thuet MJ, Philippot JR (1982) Phospholipid biosynthesis in synchronousPlasmodium falciparum cultures. J Protozool 29:258–263
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Dedicated to Prof. Dr. W. Frank on the occasion of his sixtieth birthday
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Dieckmann, A., Jung, A. Stage-specific sensitivity ofPlasmodium falciparum to antifolates. Z. Parasitenkd. 72, 591–594 (1986). https://doi.org/10.1007/BF00925479
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DOI: https://doi.org/10.1007/BF00925479