Abstract
Photosynthetic bacteria offer excellent experimental opportunities to explore both the structure and function of the ubiquinol-cytochromec oxidoreductase (bc 1 complex). In bothRhodobacter sphaeroides andRhodobacter capsulatus, thebc 1 complex functions in both the aerobic respiratory chain and as an essential component of the photosynthetic electron transport chain. Because thebc 1 complex in these organisms can be functionally coupled to the photosynthetic reaction center, flash photolysis can be used to study electron flow through the enzyme and to examine the effects of various amino acid substitutions. During the past several years, numerous mutations have been generated in the cytochromeb subunit, in the Rieske iron-sulfur subunit, and in the cytochromec 1 subunit. Both site-directed and random mutagenesis procedures have been utilized. Studies of these mutations have identified amino acid residues that are metal ligands, as well as those residues that are at or near either the quinol oxidase (Qo) site or the quinol reductase (Qi) site. The postulate that these two Q-sites are located on opposite sides of the membrane is supported by these studies. Current research is directed at exploring the details of the catalytic mechanism, the nature of the subunit interactions, and the assembly of this enzyme.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Andrews, K. M., Crofts, A. R., and Gennis, R. B. (1990).Biochemistry 29, 2645–2651.
Atta-Asafo-Adjei, E., and Daldal, F. (1991).Proc. Natl. Acad. Sci. USA 88, 492–496.
Baciou, L., Sinning, I., and Sebban, P. (1991).Biochemistry 30, 9110–9116.
Beckmann, J. D., Ljungdahl, P. O., Lopez, J. L., and Trumpower, B. L. (1987).J. Biol. Chem. 262, 8901–8909.
Brantd, U., and von Jagow, G. (1991).Eur. J. Biochem. 195, 163–170.
Brandt, U., Haase, U., Schägger, H., and von Jagow, G. (1991).J. Biol. Chem. 266, 19958–19964.
Brasseur, R. (1988).J. Biol. Chem. 263, 12571–12575.
Britt, R. D., Sauer, K., Klein, M. P., Knaff, D. B., Kriaucuinas, A., Yu, C.-A., Yu, L., and Malkin, R. (1991).Biochemistry 30, 1892–1901.
Crofts, A. R., and Wraight, C. A. (1983).Biochim. Biophys. Acta 726, 149–185.
Crofts, A., Robinson, H., Andrews, K., VanDoren, S., and Berry, E. (1987). InCatalytic Sites for Reduction and Oxidation of Quinones (Papa, S., Chance, B., and Ernster, L., eds.), Plenum, New York, pp. 617–624.
Crofts, A., Hacker, B., Barquera, B., Yun, C.-H., and Gennis, R. (1992).Biochim. Biophys. Acta 1101, 162–165.
Daldal, F., Davidson, E., and Cheng, S. (1987).J. Mol. Biol. 194, 1–12.
Daldal, F., Tokito, M. K., Davidson, E., and Faham, M. (1989).EMBO J. 8, 3951–3961.
Davidson, E., and Daldal, F. (1987a).J. Mol. Biol. 195, 25–29.
Davidson, E., and Daldal, F. (1987b).J. Mol. Biol. 195, 13–24.
Davidson, E., Ohnishi, T., Atta-Asafo-Adjei, E., and Daldal, F. (1992a).Biochemistry 31, 3342–3351.
Davidson, E., Ohnishi, T., Tokito, M., and Daldal, F. (1992b).Biochemistry 31, 3351–3358.
Degli Esposti, M., De Vries, S., Crimi, M., Ghelli, A., Patarnello, T., and Meyer, A. (1993).Biochem. Biophys. Acta, submitted.
Ding, H., Robertson, D. E., Daldal, F., and Dutton, P. L. (1992).Biochemistry 31, 3144–3158.
diRago, J. P., and Colson, A.-M. (1988).J. Biol. Chem. 263, 12564–12570.
diRago, J.-P., Perea, X., and Colson, A.-M. (1986).FEBS Lett. 208, 208–210.
diRago, J.-P., Coppée, J.-Y., and Colson, A.-M. (1989).J. Biol. Chem. 264, 14543–14548.
diRago, J.-P., Perea, J., and Colson, A.-M. (1990).FEBS. Lett. 263, 93–98.
Doyle, P. M., Li, L.-B., Yu, L., and Yu, C.-A. (1989)J. Biol. Chem. 264, 1387–1392.
Erecinska, M., Wilson, D. F., and Blasie, J. K. (1978).Biochim. Biophys. Acta 501, 63–71.
Gabellini, N. (1988).J. Bioenerg. Biomembr. 20, 59–83.
Gabellini, N., and Sebald, W. (1986).Eur. J. Biochem. 154, 569–579.
Gadsby, P. M. A., and Thomson, A. J. (1990).J. Am. Chem. Soc. 112, 5003–5011.
Gatti, D. L., Meinhardt, S. W., Ohnishi, T., and Tzagoloff, A. (1989).J. Mol. Biol. 205, 421–435.
Gerhus, E., Steinrücke, P., and Ludwig, B. (1990).J. Bacteriol. 172, 2392–2400.
Glaser, E. G., and Crofts, A. R. (1984).Biochim. Biophys. Acta 766, 322–333.
Gonzalez-Halphen, D., Vazquez-Acevedo, M., and Garcia-Ponce, B. (1991).J. Biol. Chem. 266, 3870–3876.
Graham, L. A., and Trumpower, B. L. (1991).J. Biol. Chem. 266, 22485–22492.
Gray, K. A., Davidson, E., and Daldal, F. (1992).Biochemistry 31, 11864–11873.
Güner, S., Robertson, D. E., Yu, L., Qui, Z.-h., Yu, C.-A., and Knaff, D. B. (1991).Biochim. Biophys. Acta 1058, 269–279.
Gurbiel, R. J., Batie, C. J., Sivaraja, M., True, A. E., Fee, J. A., Hoffman, B. M., and Ballou, D. P. (1989).Biochemistry 28, 4861–4871.
Gurbiel, R. J., Ohnishi, T., Robertson, D. E., Daldal, F., and Hoffman, B. M. (1991).Biochemistry 30, 11579–11584.
Hauska, G., Nitschke, W., and Herrmann, R. G. (1988).J. Bioenerg. Biomembr. 20, 211–228.
Howell, N., and Gilbert, K. (1988).J. Mol. Biol. 203, 607–618.
Howell, N., Appel, J., Cook, J. P., Howell, B., and Hauswirth, W. W. (1987).J. Biol. Chem. 262, 2411–2414.
Irie, S., Doi, S., Yorifuji, T., Takagi, M., and Yano, K. (1987).J. Bacteriol. 169, 5174–5179.
Knaff, D. B. (1992).Photosyn. Res., in press.
Konishi, K., Van Doren, S. R., Kramer, D. M., Crofts, A. R., and Gennis, R. B. (1991).J. Biol. Chem. 266, 14270–14276.
Kurkela, S., Lehväslaiho, H., Palva, E. T., and Teeri, T. H. (1988).Gene 73, 355–362.
Li, Y., DeVries, S., Leonard, K., and Weiss, H. (1981).FEBS Lett. 135, 277–280.
Meinhardt, S. W., and Crofts, A. R. (1982).FEBS Lett. 149, 217–221.
Mitchell, P. (1976).J. Theor. Biol. 62, 327–367.
Myatt, J. F., Cotton, N. P. J., and Jackson, J. B. (1987).Biochim. Biophys. Acta 890, 251–259.
Nobrega, F. G., and Tzagoloff, A. (1980).J. Biol. Chem. 255, 9828–9837.
Paddock, M. L., Feher, G., and Okamura, M. Y. (1991).Photosyn. Res. 27, 109–119.
Prince, R. C. (1983).Biochim. Biophys. Acta 723, 133–138.
Robertson, D. E., and Dutton, P. L. (1988).Biochim. Biophys. Acta 935, 273–291.
Robertson, D. E., Davidson, E., Prince, R. C., van den Berg, W., Marcs, B. L., and Dutton, P. L. (1986).J. Biol. Chem. 261, 584–591.
Robertson, D. E., Daldal, F., and Dutton, P. L. (1990).Biochemistry 29, 11249–11260.
Robertson, D. E., Ding, H., Chelminski, P. R., Slaughter, C., Hsu, J., Moonraw, C., Tokito, M., Daldal, F., and Dutton, P. L. (1993).Biochemistry 32, 1310–1317.
Robertson, D. E., Giangiacono, K. M., deVries, S., Moser, C. C., and Dutton, P.L. (1984).FEBS Lett. 178, 343–350.
Salerno, J. C., Blum, H. and Ohnishi, T. (1979).Biochim. Biophys. Acta 547, 270–281.
Saraste, M. (1984).FEBS Lett. 166, 367–372.
Schägger, H., Borchart, U., Machleidt, W., Link, T. A., and Von Jagow, G. (1987).FEBS Lett. 219, 161–168.
Simpkin, D., Palmer, G., Devlin, F. J., McKenna, M. C., Jensen, G. M., and Stephens, P. J. (1989).Biochemistry 28, 8033–8039.
Sinning, I. (1992).Trends Biochem. Sci. 17, 150–154.
Steppuhn, J., Rother, C., Hermans, J., Jansen, T., Salnikow, J., Hauska, G., and Herrmann, R. G. (1987).Mol. Gen. Genet. 210, 171–177.
Tehiler, R., and Niederman, R. A. (1991).J. Biol. Chem. 266, 23163–23168.
Tron, T., Crimi, M., Colson, A.-M., and Degli Esposti, M. (1991).Eur. J. Biochem. 199, 753–760.
Trumpower, B. L. (1990a).Microbiol. Rev. 54, 101–129.
Trumpower, B. L. (1990b).J. Biol. Chem. 265, 11409–11412.
Usui, S., and Yu, L. (1991).J. Biol. Chem. 266, 15644–15649.
Van Doren, S. R., Gennis, R. B., Barquera, B., and Crofts, A. R. (1993a).Biochemistry, in press.
Van Doren, S. R., Yun, C.-H., Crofts, A. R., and Gennis, R. B. (1993b).Biochemistry 32, 628–636.
von Jagow, G., and Ohnishi, T. (1985).FEBS Lett. 185, 311–315.
von Jagow, G., Gribble, G. W., and Trumpower, B. L. (1986).Biochemistry 25, 775–780.
Weber, S., and Wolf, K. (1988).FEBS Lett. 237, 31–34.
Widger, W. R., Cramer, W. A., Herrmann, R. G., and Trebst, A. (1984).Proc. Natl. Acad. Sci. 81, 674–678.
Yu, L., and Yu, C.-A. (1991).Biochemistry 30, 4934–4939.
Yun, C.-H., Beci, R., Crofts, A. R., Kaplan, S., and Gennis, R. B. (1990).Eur. J. Biochem. 194, 399–411.
Yun, C.-H., Crofts, A. R., and Gennis, R. B. (1991a).Biochemistry 30, 6747–6754.
Yun, C.-H., Van Doren, S. R., Crofts, A. R., and Gennis, R. B. (1991b).J. Biol. Chem. 266, 10967–10973.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Gennis, R.B., Barquera, B., Hacker, B. et al. Thebc 1 complexes ofRhodobacter sphaeroides andRhodobacter capsulatus . J Bioenerg Biomembr 25, 195–209 (1993). https://doi.org/10.1007/BF00762582
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00762582