Abstract
The disialylated poly-(N-acetyllactosamine)-containingO-linked oligosaccharide alditols, released by alkaline borohydride treatment of the enzymicallyN-deglycosylated β-subunit of equine chorionic chonadotropin, were purified by fast protein liquid chromatography (FPLC) on Mono Q and analysed by fast ion bombardment mass spectrometry (FAB-MS) and1H-NMR spectroscopy. The identified oligosaccharide alditols have the following structure:
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Abbreviations
- CG:
-
chorionic gonadotropin
- eCG:
-
equine chorionic gonadotropin
- FAB-MS:
-
fast atom bombardment mass spectrometry
- FID:
-
free induction decay
- FSH:
-
follicle stimulating hormone
- FPLC:
-
fast protein liquid chromatography
- GLC:
-
gas liquid chromatography
- Hex:
-
hexose
- HexNAc:
-
N-acetylhexosamine
- LH:
-
luteinizing hormone
- NAc:
-
N-acetyl
- Neu4,5Ac2 :
-
N-acetyl-4-O-acetylneuraminic acid
- PNGase-F:
-
peptide-N 4-(N-acetyl-β-glycosaminyl)asparagine amidase F
- WEFT:
-
water eliminated Fourier transform
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Hokke, C.H., Roosenboom, M.J.H., Thomas-Oates, J.E. et al. Structure determination of the disialylated poly-(N-acetyllactosamine)-containingO-linked carbohydrate chains of equine chorionic gonadotropin. Glycoconjugate J 11, 35–41 (1994). https://doi.org/10.1007/BF00732430
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DOI: https://doi.org/10.1007/BF00732430