Abstract
Somatic embryogenesis and plantlet formation were obtained from callus and cell suspension cultures of 40-year- old Himalayan Poplar (Populus ciliata Wall ex Royle). Callus and cell suspensions were obtained by transfer of inoculum of semiorganized leaf cultures, which were maintained on Murashige and Skoog (MS) medium supplemented with benzylaminopurine (BAP), to MS with 2,4-dichlorophenoxyacetic acid (2,4-D). Reduction of 2,4-D concentration during subsequent subculture of cell suspensions resulted in the formation of embryoids. These embryoids developed further only after being transferred to agar-based MS medium supplemented with BAP and naphthalene acetic acid. Loss of embryogenic potential was observed in cell suspensions after 6 subcultures. However, callus cultures retained the embryogenic potential even after repeated subcultures for more than a year. Plantlets could be successfully hardened and grown in natural outdoor conditions.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
Abbreviations
- BAP:
-
6-benzylaminopurine
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
- NAA:
-
1-naphthalene acetic acid
- MS:
-
Murashige and Skoog (1962) medium
References
Ahuja MR (1986) In: Evans DA, Sharp WR, Ammirato PV (eds). Handbook of Plant Cell Culture. Vol. 4. MacMillan New York, pp 626–651
Ahuja MR (1987) In: Bonga JM, Durzan DJ (eds) Cell and Tissue Culture in Forestry, Vol. 3, Martinus Nijhoff, Dordrecht, pp 207–223
Ammirato PV (1983) In: Evans DA, Sharp WR, Ammirato PV, Yamada Y (eds). Handbook of Plant Cell Culture: Vol. 1, MacMillan, New York, pp 82–123
Bajaj YPS (1986) (ed) Biotechnology in Agriculture and Forestry, Trees I, Springer-Verlag, Berlin, pp 515
Bonga JM, Durzan DJ (1987) (ed) Cell and Tissue Culture in Forestry, Vol. 3, Martinus Nijhoff, Dordrecht, pp 1–416
Cheema GS, Sharma DP (1983) Acta Hortic. 131: 75–88
Fossard De RA, Bourne RA (1976) Proc. Intl.Plant Prop.Soc. 26: 373–378
Fossard De RA (1978) In: Proc.Symp. Plant Tissue Culture, Peking, China, pp 425–438
Gupta PK, Nadgir AL, Mascarenhas AF, Jagannathan V (1980) Plant Sci. Lett. 17: 259–268
Gupta PK, Mascarenhas AF, Jagannathan V (1981) Plant Sci. Lett. 20: 265–270
LakshmiSita G, Raghava Ram NV, Vaidyanathan CS (1980) Plant Sci. Lett. 20: 63–69
Litz RE (1984) HortSci. 19: 720–722
Mehra PN, Cheema GS (1980) Phytomorph. 30: 336–343
Mehra PN, Cheema GS (1985) Phytomorph. 35: 151–154
Murashige T, Skoog F (1962) Physiol.Pl. 15: 473–497
Redenbaugh K, Paasch BD, Nichol JW, Kossler ME, Wise RR, Walker KA (1986) Bio/Technology 4: 797–801
Sondahl MR, Sharp WR (1977) Z. Pflanzenphysiol. 81: 395–408
Tisserat B, Esan EB, Murashige T (1979) Hortic. Rev. 1: 1–78
Vasil IK (1985) In: Henke RR, Hughes KW, Constanin MJ, Hollaender A (eds). Tissue Culture in Forestry and Agriculture, Plenum Press, New York, pp 31–48
Author information
Authors and Affiliations
Additional information
Communicated by H. Lörz
Rights and permissions
About this article
Cite this article
Cheema, G.S. Somatic embryogenesis and plant regeneration from cell suspension and tissue cultures of mature himalayan poplar (Populus ciliata). Plant Cell Reports 8, 124–127 (1989). https://doi.org/10.1007/BF00716855
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00716855