Abstract
Ploidy levels were calculated for callus cultures of loblolly pine (Pinus taeda L.), based on nuclear DNA content measured by Feulgen cytophotometry. The nuclear DNA content of initial stem explants showed a predominant 2C condition with less 3C and 4C, in ratios approximating those expected from diploid cells as they replicate DNA in the mitotic cell cycle. Cells with higher ploidy were produced during callus initiation, as indicated by a sharp reduction in the 2C population and a concomitant increase in higher DNA levels up to 8C. A gradual decrease in the higher ploidy levels occurred in subsequent subculture intervals, so that by 18 weeks the diploid nuclear DNA distribution was again observed, with complete elimination of DNA levels greater than 4C. Established callus cultures derived from stem or embryo explants and cultured on three different nutrient media for 48–76 weeks also showed the diploid nuclear DNA distribution with no indication of polyploid cells.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
Abbreviations
- BAP:
-
benzylaminopurine
- NAA:
-
α-naphthaleneacetic acid
- BL:
-
Brown and Lawrence's medium
- BLG:
-
modified BL medium
- LM:
-
Litvay's medium
References
Bayliss MW (1980) Int Rev Cytol, Suppl 11A:113–114
Berlyn GP, Beck TC, Renfroe MH (1986) Tree Physiol 1:227–240
Berlyn GP, Miksche JP (1976) Iowa State University Press, Ames, Iowa, 326 pp
Brown CL, Lawrence RH (1968) For Sci 14:62–64
de Torok D, White PR (1960) Science 131:730–732
Dhillon SS, Berlyn GP, Miksche JP (1977) Amer J Bot 64:117–121
Dhillon SS, Rake AV, Miksche JP (1980) Plant Physiol 65:1121–1127
Dhillon SS, Miksche JP (1983) Histochem J 15:21–37
Hakman J, von Arnold S, Bengtsson A (1984) Physiol Plant 60:321–325
Konar RN, Nagmani R (1972) Curr Sci 41:714–715
Kurth E, Gifford EM (1985) Amer J Bot 72:1676–1683
Litvay JD, Johnson MA, Verma D, Einspahr D, Weyrauch K (1981) Inst Paper Chem Techn Paper Ser 115:1–17
Mehra PN, Anand M (1979) Physiol Plant 45:127–131
Mehra PN, Anand M (1983) Physiol Plant 58:282–286
Mergen F (1959) J For 57:180–190
Miksche JP (1967) Naturwissenschaften 54:322
Miksche JP, Dhillon SS (1984) In: Vasil IK (ed), Cell Culture and Somatic Cell Genetics of Plants, Vol 1, Laboratory Procedures and Their Applications, Academic Press, NY, pp 744–751
Partanen CR (1963) Int Rev Cytol 15:215–243
Patel RK, Berlyn GP (1982) Can J For Res 12:93–101
Renfroe MH, Berlyn GP (1984) Amer J Bot 71:268–272
Renfroe MH, Berlyn GP (1985) J Plant Physiol 121:131–139
Salmia MA (1975) Physiol Plant 33:58–61
Shimada T (1971) Jap J Genet 46:235–241
Wright JW (1976) In: Introduction to Forest Genetics, Academic Press, NY, 463 pp
Author information
Authors and Affiliations
Additional information
Communicated by I. K. Vasil
Paper No. 11952 of the Journal Series of the North Carolina Agricultural Research Service, Raleigh NC 27695-7643, USA
Rights and permissions
About this article
Cite this article
Franklin, C.I., Mott, R.L. & Vuke, T.M. Stable ploidy levels in long-term callus cultures of loblolly pine. Plant Cell Reports 8, 101–104 (1989). https://doi.org/10.1007/BF00716849
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00716849