Abstract
In plants, such as maize, cyclophilin (Cyp) genes are expressed at a basal level in all tissues. Amounts of Cyp mRNA above the basic level are observed in germinating seedlings, in growing tissues/organs such as roots and leaf meristematic tissue of young maize plants, nodes and embryonic female inflorescences of adult plants and also in non-proliferating tissues such as the internodes of adult plants. Salicylic acid (SA) enhances the transcription of maize Cyp genes. The possible involvement of SA in the pathway leading to defense responses induced by abiotic stresses such as mercuric chloride treatment is discussed. A maize Cyp genomic clone isolated using a maize Cyp cDNA probe contains 737 by of the 5′ upstream and the entire coding region. This Cyp gene is not interrupted by intervening sequences. In the 5′ upstream region, characteristic transcription signals as well as putative regulatory sequences were identified. Two TATA boxes are found at positions −56 by and −66 by with respect to the transcription start site. Two putative heat shock elements were identified in the promoter region; a metal regulatory element and a third heat shock element were localized in the 5′ untranslated leader. Several putative polyadenylation signals and (G)T-rich sequence motifs were identified in the 3′ untranslated region.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, Struhl K (1987) Current protocols in molecular biology. Greene Publishing Associates and Wiley-Interscience, New York
Battling D, Heese A, Weiler E (1992) Nucleotide sequence of a cDNA encoding an Arabidopsis cyclophilin-like protein. Plant Mol Biol 19:529–530
Bergsma DJ, Eder C, Gross M, Kersten H, Sylvester D, Appelbaum E, Cusimano D, Livi GP, McLaughlin MM, Kasyan K, Porter TG, Silverman C, Dunnington D, Hand A, Prichett WP, Bossard MJ, Brandt M, Levy MA (1991) The cyclophilin multigene family of peptidyl-prolyl isomerases - characterization of three separate human isoforms. J Biol Chem 266:23204–23214
Caroni P, Rothenfluh A, McGlynn E, Schneider C (1991) S-Cyclophilin: new member of the cyclophilin family associated with the secretory pathway. J Biol Chem 266:10739–10742
Danielson PE, Forss-Petter S, Brow MA, Calavetta L, Douglass J, Milner RJ, Sutcliffe JG (1988) p1B15: a cDNA clone of the rat mRNA encoding cyclophilin. DNA 7:261–267
Dean C, Tamaki S, Dunsmuir P, Favreau M, Katayama C, Dooner H, Bedbrook J (1986) mRNA transcripts of several plant genes are polyadenylated at multiple sitesin vivo. Nucleic Acids Res 14:2229–2240
Devereux J, Haeberli P, Smithies O (1984) A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res 12:387–395
Enyedi AJ, Yalpani N, Silverman P, Raskin I (1992) Signal molecules in systemic plant resistance to pathogens and pests. Cell 70:879–886
Evans JM, Gatehouse NL, Gatehouse JA, Robinson NJ, Croy RD (1990) A pea (Pisum sativum L.) gene with homology to metallothionein. FEBS Lett 262:29–32
Feinberg AP, Vogelstein A (1983) A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 132:6–13
Fischer G, Wittmann-Liebold B, Lang K, Kiefhaber T, Schmid FX (1989) Cyclophilin and peptidyl-prolylcis-trans isomerase are probably identical proteins. Nature 337:476–478
Gasser CS, Gunning DA, Budelier KA, Brown SM (1990) Structure and expression of cytosolic cyclophilin/peptidyl-prolylcis-trans isomerase of higher plants and production of active tomato cyclophilin inE. coli. Proc Natl Acad Sci USA 87:9519–9523
Gething MJ, Sambrook J (1992) Protein folding in the cell. Nature 355:33–45
Gurley WB, Key JL (1991) Transcriptional regulation of the heat shock response: a plant perspective. Biochemistry 30:1–11
Handschumacher RE, Harding MW, Rice J, Drugge RJ (1984) Cyclophilin: a specific cytosolic binding protein for cyclosporin A. Science 226:544–546
Hasel KW, Sutcliffe LG (1990) Nucleotide sequence of a cDNA coding for mouse cyclophilin. Nucleic Acids Res 18:4019
Heitman J, Movva NR, Hall MN (1992) Proline isomerase at the crossroads of protein folding, signal transduction and immunosuppression. New Biol 4:448–460
High KP, Handschumacher RE (1992) Immunity, microbial pathogenesis, and immunophilins: finding the keys, now where are the locks? Infect Agent Dis 1:121–135
Iwai N, Inagami T (1990) Molecular cloning of a complementary DNA to rat cyclophilin-like protein mRNA. Kidney Int 37:1460–1465
Jakubowski M, Blum M, Roberts JL (1991) Postnatal development of gonadotropin-releasing hormone and cyclophilin gene expression in the female and male rat brain. Endocrinology 128:2702–2708
Joshi CP (1987a) Putative polyadenylation signals in nuclear genes of higher plants: a compilation and analysis. Nucleic Acids Res 15:9627–9640
Joshi CP (1987b) An inspection of the domain between putative TATA box and translation start site in 79 plant genes. Nucleic Acids Res 15:6643–6653
Koletsky AJ, Harding MW, Handschumacher RE (1986) Cyclophilin: distribution and variant properties in normal and neoplastic tissues. J Immunol 137:1054–1059
Malamy J, Klessig DF (1992) Salicylic acid and plant disease resistance. Plant J 2:643–654
Malamy J, Hennig J, Klessig D (1992) Temperature-dependent induction of salicylic acid and its conjugates during the resistance response to tobacco mosaic virus infection. Plant Cell 4:359–366
Marivet J, Frendo P, Burkard G (1992) Effects of abiotic stresses on cyclophilin gene expression in maize and bean and sequence analysis of bean cyclophilin cDNA. Plant Sci 84:171–178
Marty I, Brugidou C, Chartier Y, Meyer Y (1993) Growth-related gene expression inNicotiana tabacum mesophyll protoplasts. Plant J 4:265–278
Rosen KM, Villa-Komaroff L (1990) An alternative method for the visualization of RNA in formaldehyde agarose gels. Focus 12:23–24
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York
Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74:5463–5467
Sarris AH, Harding MW, Jiang TR, Aftab D, Handschumacher RE (1992) Immunofluorescent localization and immunochemical determination of cyclophilin-A with specific rabbit antisera. Transplantation 54:904–910
Schreiber SL, Crabtree GR (1992) The mechanism of action of cyclosporin-A and FK506. Immunol Today 13:136–142
Schumacher A, Schröter H, Multhaup G, Nordheim A (1991) Murine cyclophilin-S l: a variant peptidyl-prolyl isomerase with a putative signal sequence expressed in differentiating F9 cells. Biomed Biochim Acta 1129:13–22
Sorger PK (1991) Heat shock factor and the heat shock response. Cell 65:363–366
Sykes K, Gething MJ, Sambrook J (1993) Proline isomerases function during heat shock. Proc Natl Acad Sci USA 90:5853–5857
Takahashi N, Hayano T, Suzuki M (1989) Peptidyl-prolylcis-trans isomerase is the cyclosporin A-binding protein cyclophilin. Nature 337:373–375
Trandinh CC, Pao GM, Saier MH (1992) Structural and evolutionary relationships among the immunophilins: two ubiquitous families of peptidyl-prolylcis-trans isomerase. FASEB J 6:3410–3420
Walsh CT, Zydowsky LD, Mckeon FD (1992) Cyclosporin-A, the cyclophilin class of peptidylprolyl isomerases, and blockade of T-cell signal transduction. J Biol Chem 267:13115–13118
Yalpani N, Silverman P, Wilson TMA, Kleier DA, Raskin I (1991) Salicylic acid is a systemic signal and an inducer of pathogenesis-related proteins in virus-infected tobacco. Plant Cell 3:809–818
Author information
Authors and Affiliations
Additional information
Communicated by H. Saedler
The two first authors contributed equally to this work.
Rights and permissions
About this article
Cite this article
Marivet, J., Frendo, P. & Burkard, G. DNA sequence analysis of a cyclophilin gene from maize: developmental expression and regulation by salicylic acid. Molec. Gen. Genet. 247, 222–228 (1995). https://doi.org/10.1007/BF00705653
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00705653