Summary
The induction of alkane hydroxylase activity was investigated in two strains of Pseudomonas putida with a view to the production of primary alcohols. n-Nonanol production rates (16.0 μmol/g dry wt/h) with an alcohol dehydrogenase negative mutant P. putida PpS173 were considerably lower than might be expected from the growth of a wild type on n-alkane. Production of cells by fed-batch culture on n-nonane, with a specific alkane hydroxylase activity of 3.9 mmol/g/h, was considered most suitable for isolation of the alkane hydroxylase.
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Fish, N.M., Allenby, D.J. & Lilly, M.D. Oxidation of n-alkanes: Growth of Pseudomonas putida . European J. Appl. Microbiol. Biotechnol. 14, 259–262 (1982). https://doi.org/10.1007/BF00498474
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DOI: https://doi.org/10.1007/BF00498474