Abstract
Azospirillum brasilense and Arthrobacter giacomelloi were grown together in batch culture under different oxygen pressures. The response to oxygen of growth, nitrogenase activity and respiration rate was determined. The two microorganisms were found to be able to coexist all over the range of partial oxygen pressures examined, that is from 0.004–0.20 bar. Nitrogenase activity by mixed culture of A. brasilense and A. giacomelloi always appeared higher than that of A. brasilense pure culture. Low respiratory activity at partial oxygen pressures higher than 0.02 bar by both pure and mixed cultures seemed not to account for the high nitrogenase activity and improved oxygen tolerance of the mixed culture.
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Abbreviations
- pO2 :
-
partial oxygen pressure
References
Cacciari I, Lippi D, Bordeleau LM (1979) Effect of oxygen on batch and continuous cultures of a nitrogen-fixing Arthrobacter sp. Can J Microbiol 25:746–751
Cacciari I, Lippi D, Ippoliti S, Del Gallo M, Pietrosanti T, Pietrosanti W (1984) Growth and nitrogen fixation of mixed cultures of Azospirillum brasilense and Arthrobacter giacomelloi. In: Veeger C, Newton WE (eds) Advances in nitrogen fixation research. Nijhoff/Junk, Pudoc, p 329
Cacciari I, Lippi D, Ippoliti S, Pietrosanti T, Pietrosanti W (1985) Regulation of respiratory activity by oxygen in Arthrobacter fluorescens ammonium-limited chemostat culture. Can J Microbiol 31:896–899
Cacciari I, Del Gallo M, Ippoliti S, Lippi D, Pietrosanti T, Pietrosanti W (1986) Growth and survival of Azospirillum brasilense and Arthrobacter giacomelloi in binary continuous culture. Plant Soil 90:107–116
Day JM, Döbereiner J (1976) Physiological aspects of N2-fixation by a Spirillum from Digitaria roots. Soil Biol Biochem 8:45–50
Del Gallo M, Gratani L, Morpurgo G (1988) Selection at the chemostat of Azospirillum brasilense Cd N2-fixing at high O2 pressure. In: Klingmüller W (ed) Azospirillum. IV. Genetics, physiology, ecology. Springer, Berlin Heidelberg New York, pp 75–82
Drozd J, Postgate JR (1970) Effects of oxygen on acetylene reduction, cytochrome content and respiratory activity of Azotobacter chroococcum. J Gen Microbiol 63:63–73
Halsall DM, Goodchild DJ (1986) Nitrogen fixation associated with development and localization of mixed populations of Cellulomonas spp and Azospirillum brasilense grown on cellulose or wheat straw. Appl Environ Microbiol 51:849–854
Harper SHT, Lynch JM (1984) Nitrogen fixation by cellulolytic communities at aerobic-anaerobic interfaces in straw. J Appl Bacteriol 57:131–137
Hartmann A, Fu HA, Song SD, Burris RH (1985) Comparison of nitrogenase regulation in A. brasilense, A. lipoferum and A. amazonense. In: Klingmüller W (ed) Azospirillum. III. Genetics, physiology, ecology. Springer, Berlin Heidelberg New York, pp 116–126
Hurek T, Reinhold B, Fendrik I, Niemann EG (1987) Root-zone specific oxygen tolerance of Azospirillum, spp. and diazotrophic roots closely associated with Kallar grass. Appl Environ Microbiol 53:163–169
Hurek T, Reinhold B, Niemann EG, Fendrik I (1988) N2-dependent growth of Azospirillum spp in batch cultures at low concentrations of oxygen. In: Klingmüller W (ed) Azospirillum. IV. Genetics, physiology, ecology. Springer, Berlin Heidelberg New York, pp 115–121
Jagnow G (1983) Nitrogen fixation (C2H2-reduction) and growth of pure and mixed cultures of Azospirillum lipoferum, Klebsiella and Enterobacter sp. from cereal roots in liquid and semisolid media at different temperatures and oxygen concentrations. In: Klingmüller W (ed) EXS 48 Azospirillum II. Birkhäuser, Basel, pp 127–137
Kloss M, Iwannek KH, Fendrik I (1983) Physiological properties of Azospirillum brasilense sp7 in a malate-limited chemostat. J Gen Appl Microbiol 29:447–457
Love AJW, Rawson DM (1986) A note on the effects of associated microorganisms on the growth and nitrogenase activity of the cyanobacterium Anabaena cylindrica. J Appl Bacteriol 60:143–146
Lowry OH, Rosebrough NJ, Farr AJ, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275
Nelson LM, Knowles R (1978) Effect of oxygen and nitrate on nitrogen fixation and denitrification by Azospirillum brasilense grown in continous culture. Can J Microbiol 24:1395–1403
Nur I, Okon Y, Henis Y (1982) Effect of dissolved oxygen tension on production of carotenoids, poly-β-hydroxybutyrate, succinate oxidase and superoxide dismutase by Azospirillum brasilense Cd grown in continuous culture. J Gen Microbiol 128:2937–2943
Okon, Y (1985) Azospirillum: physiological properties, mode of association with roots and its application for the benefit of cereal and forage grass crops. Isr J Bot 31:214–220
Okon Y (1985) Azospirillum as a potential inoculant for agriculture. Trends Biotechnol 3:223–228
Okon Y, Albrecht SL, Burris RH (1976) Factors affecting growth and nitrogen fixation of Spirillum lipoferum. J Bacteriol 127:1248–1254
Okon Y, Houchins JP, Albrecht SL, Burris RH (1977) Growth of Spirillum lipoferum at constant partial pressures of oxygen, and the properties of its nitrogenase in cell-free extracts. J Gen Microbiol 98:87–93
Veal DA, Lynch JM (1987) Associative cellulolysis and N2 fixation by co-cultures of Trichoderma harzianum and Clostridium butyricum: the effects of ammonium-N on these processes. J Appl Bacteriol 63:245–253
Williamson JR (1963) Succinate. In: Bergmeyer HU (ed) Methods in enzymatic analysis, vol 3. Academic Press, New York, pp 1616–1621
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Cacciari, I., Lippi, D., Ippoliti, S. et al. Response to oxygen of diazotrophic Azospirillum brasilense — Arthrobacter giacomelloi mixed batch culture. Arch. Microbiol. 152, 111–114 (1989). https://doi.org/10.1007/BF00456086
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DOI: https://doi.org/10.1007/BF00456086