Summary
The genes for the initiator tRNACAU Met. tRNAUCC Gly, tRNAGGU Thr, tRNAUUC Glu and tRNAGUA Tyr and an open reading frame of 62 codons have been identified by sequencing a 2,358 by BamHl and a 1,378 by BamHI-Sst2 DNA fragments from wheat chloroplasts. A comparison of the organization of these five tRNA genes and of the open reading frame on the wheat, tobacco and spinach chloroplast genomes suggests that at least three genomic inversions must have occurred during the evolution of the wheat chloroplast genome from a spinach-like ancestor genome. Furthermore, it seems that in wheat the 91 by intergenic region between the genes for the initiator tRNAMet and the gene for tRNAUCC Gly is one end-point of the 20 kbp genomic inversion proposed by Palmer and Thompson in the case of maize (Palmer and Thompson 1982). A 119 bp duplication is located at this junction: the first copy comprises the 91 by of the intergenic region and the first 28 by of the tRNAMet gene, the second copy is found downstream of the tRNAMet gene.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Alt J, Winter P, Sebald W, Moser JG, Schedel R, Westhoff P, Herrmann RG (1983) Curr Genet 7:129–138
Alt J, Morris J, Westhoff P, Herrmann RG (1984) Curr Genet 8:597–606
Bedbrook JR, Link G, Coen DM, Bogorad L, Rich A (1978) Proc Natl Acad Sci USA 75:3060–3064
Bohnert HJ, Crouse EJ, Schmitt JM (1982) In: Parthier B, Boulter D (eds) Encyclopedia of plant physiology, nucleic acids and proteins in plant II. Springer, Berlin Heidelberg, New Series 14B:475–530
Bowman CM, Koller B, Delius H, Dyer TA (1981) Mol Gen Genet 183:93–101
Bowman CM, Bonnard G, Dyer TA (1983) Theor Appl Genet 65:247–262
Canaday J, Guillemaut P, Weil JH (1980) Nucleic Acids Res 8:999–1008
Clewell DB, Helinski DR (1969) Proc Natl Acad Sci USA 62:1159–1166
Crouse EJ, Bohnert HJ, Schmitt JM (1984) Chloroplast RNA synthesis. In: Ellis RJ (ed) Chloroplast biogenesis, Seminar Series of the Society for Experimental Biology. Cambridge University Press, 21:83–136
Deno H, Sugiura M (1983) Nucleic Acids Res 11:8407–8414
Deno H, Sugiura M (1984) Proc Natl Acad Sci USA 81:405–408
Deno H, Shinozaki K, Sugiura M (1983) Nucleic Acids Res 11:2185–2191
Ecarot-Charrier B, Cedergren RS (1976) FEBS Lett 63:287–290
Fluhr R, Edelman M (1981) Nucleic Acids Res 9:6841–6853
Holschuh K, Bottomley W, Whitfeld PR (1983) Nucleic Acids Res 11:8547–8554
Holschuh K, Bottomley W, Whitfeld PR (1984a) Plant Mol Biol 3:313–317
Holschuh K, Bottomley W, Whitfeld PR (1984b) Nucleic Acids Res 12:8819–8834
Hopp TP, Woods KR (1981) Proc Natl Acad Sci USA 78:3824–3828
Howe CJ, Auffret AD, Doherty A, Bowman CM, Dyer TA, Gray JC (1982) Proc Natl Acad Sci USA 79:6903–6907
Howe CJ, Bowman CM, Dyer TA, Gray JC (1983) Mol Gen Genet 190:51–55
Krebbers E, Steinmetz A, Bogorad L (1984) Plant Mol Biol 3:13–20
Krebbers ET (1983) Characterization of some Zea mays chloroplast genes. PhD thesis, Harvard University, Cambridge, Mass
Kung SD, Zhu YS, Shen GF (1982) Theor Appl Genet 61:73–79
Kuntz M, Weil JH, Steinmetz AA (1984) Nucleic Acids Res 12:5037–5047
Michel F, Dujon B (1983) EMBO J 2:33–38
Ohme M, Kamogashira T, Shinozaki K, Sugiura M (1984) Nucleic Acids Res 12:6741–6749
Palmer JD, Thompson WF (1982) Cell 29:537–550
Quigley F, Grienberger JM, Weil JH (1985) Plant Mol Biol 4:305–310
Rasmussen OF, Stummann BM, Henningsen KW (1984) Nucleic Acids Res 12:9143–9153
Schwarz Z, Jolly SO, Steinmetz AA, Bogorad L (1981) Proc Natl Acad Sci USA 78:3423–3427
Smith AJH (1980) Methods Enzymol 65:560–580
Sprinzl M, Gauss DH (1984) Nucleic Acids Res 12:r59-r132
Steinmetz AA, Krebbers ET, Schwarz Z, Gubbins EJ, Bogorad L (1983) J Biol Chem 258:5503–5511
Sugita M, Sugiura M (1984) Mol Gen Genet 195:308–313
Sugita M, Kato A, Shimada H, Sugiura M (1984) Mol Gen Genet 194:200–205
Vedel F, Lebacq P, Quetier F (1980) Theor Appl Genet 58:219–224
Westhoff P, Nelson N, Bunemann H, Herrmann RG (1981) Curr Genet 4:109–120
Zurawski G, Bottomley W, Whitfeld PR (1984) Nucleic Acids Res 12:6547–6558
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Quigley, F., Weil, J.H. Organization and sequence of five tRNA genes and of an unidentified reading frame in the wheat chloroplast genome: evidence for gene rearrangements during the evolution of chloroplast genomes. Curr Genet 9, 495–503 (1985). https://doi.org/10.1007/BF00434054
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00434054