Abstract
Symbiotic nitrogen fixation of Rhizobium meliloti bacteroids in Medicago sativa root nodules was suppressed by several inorganic nitrogen sources. Amino acids like glutamine, glutamic acid and aspartic acid, which can serve as sole nitrogen sources for the unnodulated plant did not influence nitrogenase activity of effective nodules, even at high concentrations.
Ammonia and nitrate suppressed symbiotic nitrogen fixation in vivo only at concentrations much higher than those needed for suppression of nitrogenase activity in free living nitrogen fixing bacteria. The kinetics of suppression were slow compared with that of free living nitrogen fixing bacteria. On the other hand, nitrite, which acts as a direct inhibitor of nitrogenase, suppressed very quickly and at low concentrations. Glutamic acid and glutamine enhanced the effect of ammonia dramatically, while the suppression by nitrate was enhanced only slightly.
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References
Brown, C. M., Dilworth, M. J.: Ammonia assimilation by Rhizobium cultures and bacteriods. J. gen. Microbiol. 86, 39–48 (1975)
Copeland, R., Pate, J. S.: Nitrogen metabolism of nodulated white clover in the presence and absence of nitrate nitrogen. Occasional symposium No. 6 of the British Grassland Society pp. 71–77, British Grassland Society. Hurley, Berkshire (1970)
Dart, P. J., Wildon, D. C.: Nodulation and nitrogen fixation by Vigna sinensis and Vicia atropurpurea: The influence of concentration, form and site of application of combined nitrogen. Austr. J. Agric. Res. 21, 45–56 (1969)
Drozd, J. W., Tubb, R. S., Postgate, J. R.: A chemostat study of the effect of fixed nitrogen sources on nitrogen fixation, membranes and free amino acids in Azotobacter chroococcum. J. gen. Microbiol. 73, 221–232 (1972)
Gibson, A. H., Nutman, P. S.: Studies on the physiology of nodule formation. VII. A reappraisal of the effect of preplanting. Ann. Bot. 24, 420–433 (1960)
Hardy, R. W. F., Burns, R. C., Holsten, R. D.: Application of the acetylene-ethylene assay for measurement of nitrogen fixation. Soil Biol. Biochem. 5, 47–81 (1973)
Hardy, R. W. F., Holsten, R. D., Jackson, E. K., Burns, R. C.: The acetylene-ethylene assay for N2-fixation: Laboratory and field evaluation. Plant Physiol. 43, 1185–1207 (1968)
Jensen, H. L.: Nitrogen fixation in leguminous plants. I. General characters of root nodule bacteria isolated from species of Medicago and Trifolium in Australia. Proc. Linn. Soc. N.S.W. 66, 98–108 (1942)
Keister, D. J.: Acetylene reduction by pure cultures of Rhizobia. J. Bacteriol. 123, 1265–1268 (1975)
Kennedy, I. R.: Primary products of symbiotic nitrogen fixation. II. Pulse-labelling of seradella nodules with 15N2. Biochim. Biophys. Acta. 130, 295–303 (1966)
Kennedy, I. R., Rigaud, J., Trichant, J. C.: Nitrate reductase from bacteriods of Rhizobium japonicum: Enzyme characteristics and possible interactions with nitrogen fixation. Biochim. Biophys. Acta. 397, 24–35 (1975)
Kleiner, D.: Quantitative relations for the repression of nitrogenase synthesis in Azotobacter vinelandii by ammonia. Arch. Microbiol. 101, 153–159 (1974)
Kurz, W. G. W., Rokosh, D. A., LaRue, T. A.: Enzymes of ammonia assimilation in Rhizobium leguminosarum bacteroids. Can. J. Microbiol. 21, 1009–1012 (1975)
Munns, D. N.: Nodulation of Medicago sativa in solution culture. II. Compensating effects of nitrate and of prior nodulation. Plant Soil. 28, 246–257 (1968)
Pate, J. S., Dart, P. J.: Nodulation studies in legumes. IV. The influence of inoculum strain and time of application of ammonium nitrate on symbiotic response. Plant Soil. 15, 329–346 (1961)
Rigaud, J., Bergersen, F. J., Turner, G. L., Daniel, R. M.: Nitrate dependent anaerobic acetylene-reduction and nitrogen-fixation by soybean bacteriods. J. gen. Microbiol. 77, 137–144 (1973)
Robertson, J. G., Scott, D. B., Farnden, K. J. F.: Ammonia assimilation in lupin nodules. Nature 263, 703–705 (1976)
Robertson, J. G., Warburton, M. P., Farnden, K. J. F.: Induction of glutamate synthase during nodule development in lupin. FEBS Letters 55, 33–37 (1975)
Ryan, E., Fortrell, P. F.: Subcellular localization of enzymes involved in the assimilation of ammonia by soybean root nodules. Phytochemistry 13, 2647–2652 (1974)
Schmidt, G.: On the effect of environmental conditions, especially the nitrogen nutrition, on nodulation and N-linkage of Vicia faba L. Kühn-Arch. 69, 163–222 (1955)
Sorger, G. J.: Regulation of nitrogen fixation in Azotobacter vinelandii OP: The role of nitrate reductase. J. Bacteriol 98, 56–61 (1969)
Van Straten, J., Schmidt, E. L.: Action of water in depressing acetylene reduction by detached nodules. Appl. Microbiol. 29, 432–434 (1975)
Tubb, R. S., Postgate, J. R.: Control of nitrogenase synthesis in Klebsiella pneumoniae. J. gen. Microbiol. 79, 103–117 (1973)
Wilson, P. W.: The biochemistry of symbiotic nitrogen fixation, pp. 114–141. Madison, Wisconsin: The Univ. of Wisconsin Press 1940
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Kamberger, W. Regulation of symbiotic nitrogen fixation in root nodules of alfalfa (Medicago sativa) infected with Rhizobium meliloti . Arch. Microbiol. 115, 103–108 (1977). https://doi.org/10.1007/BF00427852
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DOI: https://doi.org/10.1007/BF00427852