Abstract
Repeated sessions of electrical stimulation or lesions in the ventral aspect of the medial forebrain bundle (VMFB) region of the brain in rats resulted in a significant increase and a decrease in voluntary ethanol (10% v/v) intake, respectively. Whole brain and midbrain-diencephalon (MB-DE) aldehyde dehydrogenase (ALDH) were measured in different groups of experimental and control animals before, immediately after, and 30 days after the termination of the stimulation regimen, or 8 days and 30 days after the induction of the lesions. By the end of the stimulation regimen, the levels of MB-DE ALDH of the experimental (ethanol-drinking: stimulated) animals were significantly higher than those of control animals (ethanol-drinking: nonstimulated, water-drinking: stimulated, and water-drinking: nonstimulated). A marked decrease in MB-DE ALDH activity was noted in lesioned animals but not in cyanamide-treated or in implanted control animals. Neither the stimulation nor the lesions had any demonstrable effect on whole brain ALDH activity. Cyanamide administration caused a pronounced decrease in ethanol intake and in levels of liver ALDH activity. The increase in MB-DE ALDH activity in the ethanol-drinking, stimulated animals was attributed to the interaction between the VMFB activation and the ethanol drinking, while the reduction in ALDH activity was attributed to the degeneration of biogenic-amine-containing nerve fibers.
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Agid, Y., Javoy, F., Youdim, M. B. H.: Monoamine oxidase and aldehyde dehydrogenase activity in the striatum of rats after 6-hydroxydopamine lesion of the nigrostriatal pathway. Br. J. Pharmacol. 48, 175–178 (1973)
Alousi, A., Weiner, N.: The regulation of norepinephrine synthesis in sympathetic nerve. Effect of nerve stimulation, cocaine and catecholamine releasing agents. Proc. Nat. Acad. Sci. (U.S.A.) 56, 1491–1496 (1966)
Amir, S.: Brain and liver aldehyde dehydrogenase: relations to ethanol consumption in Wistar rats. Neuropharmacology 16, 781–784 (1977)
Amir, S.: Brain and liver aldehyde dehydrogenase activity and voluntary ethanol consumption by rats: relations to strain, sex and age. Psychopharmacology (in press, 1978)
Amit, Z., Stern, M. H., Wise, R. A.: Alcohol preference in the laboratory rat induced by hypothalamic stimulation. Psychopharmacologia (Berl.) 17, 367–377 (1970)
Amit, Z., Stern, M. H.: A further investigation of alcohol preference in the laboratory rat induced by hypothalamic stimulation. Psychopharmacologia (Berl.) 21, 317–327 (1971)
Amit, Z., Stern, M. H.: Electrochemical interactions in the medial forebrain bundle and alcohol dependence in the laboratory rat. In: Biological aspects of alcohol consumption, vol. 20, O. Forsander and K. Ericksson, eds., pp. 225–231. Helsinki: Finnish Found 1972
Amit, Z., Meade, R. G., Levitan, D. E., Singer, J.: Effects of dorsal and ventral lateral hypothalamic lesions on voluntary alcohol consumption by rats. J. Stud. Alc. 37, 1188–1196 (1976)
Cohen, G.: Alkaloid products in the metabolism of alcohol and biogenic amines. Biochem. Pharmacol. 25, 1123–1128 (1976)
Consbruch, U., Derwort, A.: Klinische Erfahrungen und biochemische Aspekte bei der Behandlung des Alkoholismus mit Calciumcarbimid. Int. Pharmacopsychiatry 1, 143–157 (1968)
Deitrich, R. A.: Tissue and subcellular distribution of mammalian aldehyde oxidizing capacity. Biochem. Pharmacol. 15, 1911–1922 (1966)
Deitrich, R. A.: Biochemical aspects of alcoholism. Psychoneuroendocrinology 1, 325–346 (1976)
Deitrich, R. A., Erwin, V. G.: Involvement of biogenic amine metabolism in ethanol addiction. Fed. Proc. 34, 1962–1968 (1975)
Deitrich, R. A., Troxell, P. A., Worth, W. S., Erwin, V. G.: Inhibition of aldehyde dehydrogenase in brain and liver by cyanamide. Biochem. Pharmacol. 25, 2733–2737 (1976)
Duncan, R. J. S., Tipton, K. F.: The purification and properties of NAD-linked aldehyde dehydrogenase from pig brain. Eur J. Biochem. 22, 257–262 (1971)
Duncan, R. J. S., Sourkes, T. L., Boucher, R., Poirier, L. J., Roberge, A.: Aldehyde dehydrogenase and monoamine oxidase in the striatum of cats with nigrostriatal lesions. J. Neurochem. 19, 2007–2010 (1972)
Duncan, R. J. S., Sourkes, T. L.: Some enzymic aspects of the production of oxidized or reduced metabolites of catecholamines and 5-hydroxytryptamine by brain tissue. J. Neurochem. 22, 663–669 (1974)
Eriksson, K.: Genetic selection for voluntary alcohol consumption in the albino rat. Science 159, 739–741 (1968)
Erwin, V. G., Deitrich, R. A.: Brain aldehyde dehydrogenase localization, purification and properties. J. Biol. Chem. 241, 3533–3539 (1966)
German, D. C., Bowden, B. M.: Catecholamine systems as to neural substrate for intracranial self-stimulation: a hypothesis. Brain Res. 73, 381–419 (1974)
Israel, Y., Mardones, J.: Biological basis of alcoholism, p. 326. New York: Wiley, Interscience 1971
Kopin, I. J.: Regulation of catecholamine-synthesizing enzymes. J. Psychiatr. Res. 11, 335–338 (1974)
Lahti, R. A., Majchrowicz, E.: Acetaldehyde—an inhibitor of enzymatic oxidation of 5-hydroxy indole acetaldehyde. Biochem. Pharmacol. 18, 535–538 (1969)
Lowry, O. H., Rosebrough, N. J., Farr, A. L., Randall, R. J.: Protein measurement with the folin phenol reagent. J. Biol. Chem. 193, 265–275 (1951)
Melchior, C. L., Myers, R. D.: Preference for alcohol evoked by tetrahydropapaveroline (THP) chronically infused in the cerebral ventricle of the rat. Pharmacol. Biochem. Behav. 7, 19–35 (1977)
Miller, R. G.: Simultaneous statistical inference. New York: McGraw-Hill 1966
Mogenson, G. J., Phillips, A. G.: Motivation: a psychological construct in search of a physiological substrate. In: Progress in psychobiology and physiological psychology, J. M. Sprague and A. N. Epstein, eds., pp. 189–244. New York: Academic Press 1976
Murrin, L. C., Roth, R. H.: Dopaminergic neurons: effects of stimulation on dopamine metabolism. Pharmacologist 15, 514 (1973)
Myers, R. D., Evans, J. E., Yaksh, T. L.: Ethanol preference in the rat: interactions between brain serotonin and ethanol, acetaldehyde, paraldehyde 5-HTP and 5-HTOL. Neuropharmacology 11, 539–549 (1972)
Myers, R. D., Melchior, C. L.: Alcohol drinking: abnormal intake caused by tetrahydropapaveroline in brain. Science 196, 554–556 (1977)
Myers, R. D., Veale, W. L.: Alternations in volitional alcohol intake produced in rats by chronic intraventricular infusions of acetaldehyde, paraldehyde or methanol. Arch. Int. Pharmacol. 180, 100–113 (1969)
Pellegrino, L. J., Cushman, A. J.: A stereotaxic atlas of the rat brain. New York: Appleton-Century-Crofts 1967
Pettersson, H., Kiessling, K. H.: Acetaldehyde occurrence in cerebrospinal fluid during ethanol oxidation in rats and its dependence on the blood level and on dietary factors. Biochem. Pharmacol. 26, 237–240 (1977)
Rahwan, R. G.: Toxic effects of ethanol: possible role of acetaldehyde, tetrahydroisoquinolines, and tetrahydrocarbolines. Toxicol. Appl. Pharmacol. 34, 3–27 (1975)
Russell, K. E., Stern, M. H.: Sex and strain as factors in voluntary alcohol intake. Physiol. Behav. 10, 641–642 (1973)
Sippel, H. W.: The acetaldehyde content in rat brain during ethanol metabolism. J. Neurochem. 23, 451–452 (1974)
Tabakoff, B., Anderson, R. A., Ritzmann, R. F.: Brain acetaldehyde after ethanol administration. Biochem. Pharmacol. 25, 1305–1309 (1976)
Ungerstedt, U.: Stereotaxic mapping of the monoamine pathways in the rat brain. Acta Physiol. Scand. [Suppl.] 367, 1–48 (1971)
von Wartburg, J. P., Berger, P., Ris, M. M., Tabakoff, B.: Enzymes of biogenic aldehyde metabolism. In: Alcohol intoxication and withdrawal, M. M. Gross, ed., pp. 119–138 New York: Plenum 1975
Wallgren, H., Barry, H., III: Actions of alcohol, vol. II, p. 419. Amsterdam and New York: Elsevier 1970
Walters, D. S., Eccleston, D.: Increase noradrenaline metabolism following electrical stimulation of the locus coeruleus in the rat. J. Neurochem. 21, 281–289 (1973)
Wayner, M. J., Greenberg, I., Carey, R. J., Nolley, D.: Ethanol drinking elicited during electrical stimulation of the lateral hypothalamus. Physiol. Behav. 7, 793–795 (1971)
Wayner, M. J., Greenberg, I., Tartaglione, R., Nolley, D., Fraley, S., Cott, A.: A new factor affecting the consumption of ethyl alcohol and other sapid fluids. Physiol. Behav. 8, 345–362 (1972)
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Amir, S., Stern, M.H. Electrical stimulation and lesions of the medial forebrain bundle of the rat: Changes in voluntary ethanol consumption and brain aldehyde dehydrogenase activity. Psychopharmacology 57, 167–174 (1978). https://doi.org/10.1007/BF00426883
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DOI: https://doi.org/10.1007/BF00426883