The lateral septum (LS) is an important part of the limbic and reward brain systems; its activity has been associated with the development of different aspects of addiction. Using a stereotaxically inserted semi-microelectrode, we recorded local field potentials (LFPs) from the rat LS under background conditions and after i.p. injection of 2.0 g/kg body mass 15% ethanol (Ethl) solution. The recorded LFPs showed typical 1/f behavior. Acute Ethl intoxication resulted in increases in the spectral power of nearly all frequency LFP ranges, except the beta-1 sub-band. A prominent peak has been detected in the beta-1 sub-band centered at 14.77 Hz. Ethanol intoxication resulted in a shift of this peak toward 13.70 Hz with simultaneous decrease in the power of the above sub-band. Powers of most LFP ranges/subranges under conditions of the above intoxications demonstrated increments, most intense for delta, beta-2, gamma-high, and epsilon (ultrafast, >90 Hz) oscillations. Changes in the relatively highfrequency LFP components are assumed to reflect Ethl-induced modifications of the activity of local LS neuronal ensembles, while those of low-frequency (delta) oscillations are probably related to global modulation of the activity of the entire brain. It is supposed that the observed changes caused by acute Ethl intoxication may reflect mainly intensification of GABA-mediated signaling.
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References
V. H. Murthy, “Facing addiction in the United States: Surgeon general’s report on alcohol, drugs, and health,” JAMA, 317, No. 2, 133–134 (2017); https://doi.org/10.1001/jama.2016.18215
G. F. Koob and N. D. Volkow, “Neurocircuitry of addiction,” Neuropsychopharmacology, 35, No. 1, 217– 238 (2010); https://doi.org/10.1038/npp.2009.110
K. Deng, L. Yang, J. Xie, et al., “Whole-brain mapping of projection from mouse lateral septal nucleus,” Biol. Open, 8, No. 7, bio043554 (2019).
M. F. Gárate-Pérez, A. Méndez, C. Bahamondes, et al., “Vasopressin in the lateral septum decreases conditioned place preference to amphetamine and nucleus accumbens dopamine release,” Addict. Boil., 26, No. 1, e12851 (2021); 10.1111/adb.12851
G. C. Sartor and G. S. Aston-Jones, “A septal-hypothalamic pathway drives orexin neurons, which is necessary for conditioned cocaine preference,” J. Neurosci., 32, No. 13, 4623–4631 (2012); https://doi.org/10.1523/JNEUROSCI.4561-11.2012
G. Buzsáki, C. A. Anastassiou, and C. Koch, “The origin of extracellular fields and currents--EEG, ECoG, LFP and spikes,” Nat. Rev. Neurosci., 13, No. 6, 407–420 (2012); https://doi.org/10.1038/nrn3241
S. Katzner, I. Nauhaus, A. Benucci, et al., “Local origin of field potentials in visual cortex,” Neuron, 61, No. 1, 35–41 (2009); https://doi.org/10.1016/j.neuron.2008.11.016
W. J. Pan, G. J. Thompson, M. E. Magnuson, et al., “Infraslow LFP correlates to resting-state fMRI BOLD signals,” NeuroImage, 74, 288–297 (2013); 10.1016/j.neuroimage.2013.02.035
D. D. Wang, C. de Hemptinne, S. Miocinovic, et al., “Subthalamic local field potentials in Parkinson’s disease and isolated dystonia: An evaluation of potential biomarkers,” Neurobiol. Dis., 89, 213–222 (2016); 10.1016/j.nbd.2016.02.015
M. Chaturvedi, F. Hatz, U. Gschwandtner, et al., “Quantitative EEG (QEEG) measures differentiate Parkinson’s disease (PD) patients from healthy controls (HC),” Front. Aging Neurosci., 9, 3 (2017); 10.3389/fnagi.2017.00003
F. Maestú, P. Cuesta, O. Hasan, et al., “The importance of the validation of M/EEG with current biomarkers in Alzheimer’s disease,” Front. Hum. Neurosci., 13, 17 (2019); 10.3389/fnhum.2019.00017
J. Voges, U. Müller, B. Bogerts, et al., “Deep brain stimulation surgery for alcohol addiction,” World Neurosurg., 80, No. 3–4, S28.e21–S28.e31 (2013); 10.1016/j.wneu.2012.07.011
V. Di Lazzaro, G. Pellegrino, F. Ranieri, et al., “Effects of repetitive TMS of the motor cortex on disease progression and on glutamate and GABA levels in ALS: A proof of principle study,” Brain Stimul., 10, No. 5, 1003–1005 (2017); https://doi.org/10.1016/j.brs.2017.05.003
T. Tsurugizawa, Y. Abe, and D. Le Bihan, “Water apparent diffusion coefficient correlates with gamma oscillations of local field potentials in the rat brain nucleus accumbens following alcohol injection,” J.Cereb. Blood Flow Metab., 37, No. 9, 3193–3202 (2017); https://doi.org/10.1177/0271678X16685104
A. M. Henricks, E. Sullivan, L. L. Dwiel, et al., “Sex differences in the ability of corticostriatal oscillations to predict rodent alcohol consumption,” Biol. Sex Differ., 10, No. 1, 61 (2019); 10.1186/s13293-019-0276-0
H. Cruces-Solis, O. Babaev, H. Ali, et al., “Altered theta/beta frequency synchrony links abnormal anxietyrelated behavior to synaptic inhibition in Neuroligin-2 knockout mice,” BioRxiv, 726190 (2019); https://doi.org/10.1101/726190
K. Miyake, S. Yagi, Y. Aoki, et al., “Acute effects of ethanol on hippocampal spatial representation and offline reactivation,” Front. Cell. Neurosci., 14, 571175 (2020); 10.3389/fncel.2020.571175
T. L. Doremus-Fitzwater, H. M. Buck, K. Bordner, et al., “Intoxication- and withdrawal-dependent expression of central and peripheral cytokines following initial ethanol exposure,” Alcoholism: Clin. Exp. Res., 38, No. 8, 2186–2198 (2014); https://doi.org/10.1111/acer.12481
O. Karlsson and E. Roman, “Dose-dependent effects of alcohol administration on behavioral profiles in the MCSF test,” Alcohol, 50, 51–56 (2016); 10.1016/j.alcohol.2015.10.003
G. Paxinos and C. Watson, The Rat Brain in Stereotaxic Coordinates, 6th edition, Elsevier, Academic Press (2006).
V. M. Moroz, O. V. Vlasenko, I. L. Rokunets, et al., “Coupled spike activity in micropopulations of motor cortex neurons in rats,” Neurophysiology, 42, No. 2, 110–117 (2010).
O. Chaikovska, O. Ponomarenko, O. Dovgan, et al., “Concept and realization of back-pack type system for multichannel electrophysiology in freely behaving rodents,” Informatics Control Measurement in Economy and Environmental Protection, 9, No. 4, 64–68 (2019); 10.35784/iapgos.688
E. Pérez-Garci, Y. del Río-Portilla, M. A. Guevara, et al., “Paradoxical sleep is characterized by uncoupled gamma activity between frontal and perceptual cortical regions,” Sleep, 24, N. 1, 118–126 (2001); https://doi.org/10.1093/sleep/24.1.118
M. A. A. van der Meer and A. D. Redish, “Low and high gamma oscillations in rat ventral striatum have distinct relationships to behavior, reward, and spiking activity on a learned spatial decision task,” Front. Integr. Neurosci., 3, 9 (2009); 10.3389/neuro.07.009.2009
W. Jing, Y. Wang, et al., “EEG bands of wakeful rest, slow-wave and rapid-eye-movement sleep at different brain areas in rats,” Front. Comput. Neurosci., 10, 79 (2016); 10.3389/fncom.2016.00079
P. Welch, “The use of fast Fourier transform for the estimation of power spectra: a method based on time averaging over short, modified periodograms,” IEEE Trans. Audio Electroacoust., 15, No. 2, 70–73 (1967).
M. X. Cohen, “A better way to define and describe Morlet wavelets for time-frequency analysis,” NeuroImage, 199, 81–86 (2019); 10.1016/j.neuroimage.2019.05.048
Y. Zhou, A. Sheremet, Y. Qin, et al., “Methodological considerations on the use of different spectral decomposition algorithms to study hippocampal rhythms,” eNeuro, 6, No. 4, ENEURO.0142-19.2019 (2019); 10.1523/ENEURO.0142-19.2019
M. X. Cohen, Analyzing Neural Time Series Data: Theory and Practice, MIT Press (2014).
M. C. Ng, J. Jing, and M. B. Westover, Atlas of Intensive Care Quantitative EEG, Springer (2019).
N. Lotfullina and R. Khazipov, “Ethanol and the developing brain: inhibition of neuronal activity and neuroapoptosis,” Neuroscientist, 24, No. 2, 130–141 (2018); https://doi.org/10.1177/1073858417712667
K. P. Abrahao, M. J. Pava, and D. M. Lovinger, “Dosedependent alcohol effects on electroencephalogram: Sedation/anesthesia is qualitatively distinct from sleep,” Neuropharmacology, 164, 107913 (2020); 10.1016/j.neuropharm.2019.107913
C. Kamarajan, B. Porjesz, K. A. Jones, et al., “The role of brain oscillations as functional correlates of cognitive systems: a study of frontal inhibitory control in alcoholism,” Int. J. Psychophysiol., 51, No. 2, 155–180 (2004); https://doi.org/10.1016/j.ijpsycho.2003.09.004
J. R. Manning, J. Jacobs, I. Fried, and M. J. Kahana, “Broadband shifts in local field potential power spectra are correlated with single-neuron spiking in humans,” J. Neurosci., 29, No. 43, 13613–13620 (2009); https://doi.org/10.1523/JNEUROSCI.2041-09.2009
B. Porjesz and H. Begleiter, “Alcoholism and human electrophysiology,” Alcohol Res. Health, 27, No. 2, 153–160 (2003).
M. Rangaswamy and B. Porjesz, “Understanding alcohol use disorders with neuroelectrophysiology,” Handb. Clin. Neurol., 125, 383–414 (2014); 10.1016/B978-0-444-62619-6.00023-9
M. Rangaswamy, B. Porjesz, D. Chorlian, et al., “Theta power in the EEG of alcoholics,” Alcohol. Clin. Exp. Res., 27, No. 4, 607–615 (2003); https://doi.org/10.1097/01.ALC.0000060523.95470.8F
J. R. Criado and C. L. Ehlers, “Effects of adolescent ethanol exposure on event-related oscillations (EROs) in the hippocampus of adult rats,” Behav. Brain Res., 210, No. 2, 164–170 (2010); https://doi.org/10.1016/j.bbr.2010.02.021
B. Givens “Low doses of ethanol impair spatial working memory and reduce hippocampal theta activity,” Alcoholism: Clin. Exp. Res., 19, No. 3, 763–767 (1995); https://doi.org/10.1111/j.1530-0277.1995.tb01580.x
A. E. Campbell, P. Sumner, K. D. Singh, and S. D. Mu-thukumaraswamy, “Acute effects of alcohol on stimu-lus-induced gamma oscillations in human primary visual and motor cortices,” Neuropsychopharmacology, 39, No. 9, 2104–2113 (2014); https://doi.org/10.1038/npp.2014.58
C. F. Valenzuela, “Alcohol and neurotransmitter interactions,” Alcohol Health Res. World, 21, No. 2, 144–148 (1997).
S. Kumar, P. Porcu, D. E. Werner, et al., “The role of GABAA receptors in the acute and chronic effects of ethanol: a decade of progress,” Psychopharmacology, 205, No. 4, 529–564 (2009); https://doi.org/10.1007/s00213-009-1562-z
T. J. Baumgarten, G. Oeltzschner, N. Hoogenboom, et al., “Beta peak frequencies at rest correlate with endogenous GABA+/Cr concentrations in sensorimotor cortex areas,” PloS One, 11, No. 6, e0156829 (2016); 10.1371/journal.pone.0156829
B. L. Osinski, A. Kim, W. Xiao, et al., “Pharmacological manipulation of the olfactory bulb modulates beta oscillations: testing model predictions,” J. Neurophysiol., 120, No. 3, 1090–1106 (2018); https://doi.org/10.1152/jn.00090.2018
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Chaikovska, O.V., Rokunets, I.L., Dovhan, O.V. et al. Effects of Acute Ethanol Intoxication on Local Field Potentials in the Rat Lateral Septum. Neurophysiology 53, 30–40 (2021). https://doi.org/10.1007/s11062-021-09910-8
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DOI: https://doi.org/10.1007/s11062-021-09910-8