Summary
Tobacco mosaic virus (TMV) is a message-sense, single-stranded RNA virus that infects many Solanaceae plants. A full-length cDNA copy of TMV genomic RNA was constructed and introduced into the genomic DNA of tobacco plants using a disarmed Ti plasmid vector. Transformed plants showed typical symptoms of TMV infection, and their leaves contained infectious TMV particles. This is the first example of the expression of RNA virus genomic RNAs in planta.
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References
Ahlquist P, Janda M (1984) cDNA cloning and in vitro transcription of the complete brome mosaic virus genome. Mol Cell Biol 4:2876–2882
Ahlquist P, French R, Janda M, Loesch-Fries LS (1984) Multicomponent RNA plant virus infection derived from cloned viral cDNA. Proc Natl Acad Sci USA 81:7066–7070
Baulcombe DC, Saunders GR, Bevan MW, Mayo MA, Harrison BD (1986) Expression of biologically active viral satellite RNA from the nuclear genome of transformed plants. Nature 321:446–449
Dawson WO, Beck DL, Knorr DA, Grantham GL (1986) cDNA cloning of the complete genome of tobacco mosaic virus and production of infectious transcripts. Proc Natl Acad Sci USA 83:1832–1836
Franck A, Guilley H, Jonard G, Richards K, Hirth L (1980) Nucleotide sequence of cauliflower mosaic virus DNA. Cell 21:285–294
Galbraith DW, Harkins KR, Maddox JM, Ayers NM, Sharma DP, Firoozabady E (1983) Rapid flow cytometric analysis of the cell cycle in intact plant tissue. Science 220:1049–1051
Garcia JA, Hille J, Vos P, Goldbach R (1987) Transformation of cowpea Vigna unguiculata with a full-length DNA copy of cowpea mosaic virus M-RNA. Plant Sci 48:89–98
Gardner RC, Chonoles KR, Owens RA (1986) Potato spindle tuber viroid infections mediated by the Ti plasmid of Agrobacterium tumefaciens. Plant Mol Biol 6:221–228
Goeddel D, Shepard HM, Yelverton E, Leung D, Crea R (1980) Synthesis of human fibroblast interferon by E. coli. Nucleic Acids Res 8:4057–4074
Grimsley N, Hohn B, Hohn T, Walden R (1986) “Agroinfection” an alternate route for viral infection of plants by using the Ti plasmid. Proc Natl Acad Sci USA 83:3282–3286
Grimsley N, Hohn T, Davies JW, Hohn B (1987) Agrobacterium-mediated delivery of infectious maize streak virus into maize plants. Nature 325:177–179
Guilley H, Dudley RK, Jonard G, Balàzs E, Richards KE (1982) Transcription of cauliflower mosaic virus DNA: Detection of promoter sequences, and characterization of transcripts. Cell 30:763–773
Guo LH, Wu R (1982) New rapid methods for DNA sequencing based on exonuclease III digestion followed by repair synthesis. Nucleic Acids Res 10:2065–2084
Hain R, Stabel R, Czernilofsky AP, Steinbiß HH, Herrera-Estrella L, Schell J (1985) Uptake, integration, expression and genetic transmission of a selectable chimaeric gene by plant protoplasts. Mol Gen Genet 199:161–168
Hattori M, Sakaki Y (1986) Dideoxy sequencing method using denatured plasmid templates. Anal Biochem 152:232–238
Horsch RB, Fry JE, Hoffmann NL, Eichholtz D, Rogers SG, Fraley RT (1985) A simple and general method for transferring genes into plants. Science 227:1229–1231
Ishikawa M, Meshi T, Motoyoshi F, Takamatsu N, Okada Y (1986) In vitro mutagenesis of the putative replicase genes of tobacco mosaic virus. Nucleic Acids Res 14:8291–8305
Meshi T, Ishikawa M, Motoyoshi F, Semba K, Okada Y (1986) In vitro transcription of infectious RNAs from full-length cDNAs of tobacco mosaic virus. Proc Natl Acad Sci USA 83:5043–5047
Odell JT, Nagy F, Chua NH (1985) Identification of DNA sequences required for activity of the cauliflower mosaic virus 35 S promoter. Nature 313:810–812
Ohno T, Aoyagi M, Yamanashi Y, Saito H, Ikawa S, Meshi T, Okada Y (1984) Nucleotide sequence of the tobacco mosaic virus (tomato strain) genome and comparison with the common strain genome. J Biochem 96:1915–1923
Paszkowski J, Shillito RD, Saul M, Mandák V, Hohn T, Hohn B, Potrykus I (1984) Direct gene transfer to plants. EMBO J 3:2717–2722
Reed CR, Mann DA (1985) Rapid transfer of DNA from agarose to nylon membranes. Nucleic Acids Res 13:7207–7221
Rogers SG, Bisaro DM, Horsch RB, Fraley RT, Hoffmann NL, Brand L, Elmer JS, Lloyd AM (1986) Tomato golden mosaic virus A component DNA replicates automonously in transgenic plants. Cell 45:593–600
Takamatsu N, Ishikawa M, Meshi T, Okada Y (1987) Expression of bacterial chloramphenicol acetyltransferase gene in tobacco plants mediated by TMV-RNA. EMBO J 6:307–311
Van Haute E, Joos H, Maes M, Warren G, Van Montagu M, Schell J (1983) Intergenic transfer and exchange recombination of restriction fragments cloned in pBR322: a novel strategy for the reversed genetics of the Ti plasmids of Agrobacterium tumefaciens. EMBO J 2:411–417
Zambryski P, Joos H, Genetello C, Leemans J, Van Montagu M, Schell J (1983) Ti plasmid vector for the introduction of DNA into plant cells without alteration of their normal regeneration capacity. EMBO J 2:2143–2150
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Yamaya, J., Yoshioka, M., Meshi, T. et al. Expression of tobacco mosaic virus RNA in transgenic plants. Molec. Gen. Genet. 211, 520–525 (1988). https://doi.org/10.1007/BF00425710
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DOI: https://doi.org/10.1007/BF00425710