Summary
The genome structures of a temperate Lactobacillus phage, ϕFSW, and its virulent mutants, ϕFSVs, were examined by restriction, heteroduplex and nucleotide-sequence analyses. The results showed that two out of three ϕFSVs had the same 1.3 kbp insertion (designated as ISL1) at different positions in the ϕFSW sequence. ISL1 was 1,256 bp long and contained at least two long open reading frames of 279 and 822 bases on one strand. Inverted repeats were found at the termini of the ISL1 which was bracketed by 3 bp direct repeats of the ϕFSW sequence. From this evidence, we concluded that ISL1 was a transposable element in Lactobacillus casei.
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References
Auerswald E-A, Ludwig G, Schaller H (1981) Structural analysis of Tn5. Cold Spring Harbor Symp Quant Biol 45:107–113
Bernardi A, Bernardi F (1981) Complete sequence of an IS element present in pSC101. Nucleic Acids Res 9:2905–2911
DasSarma S, RajBhandary UL, Khorana HG (1983) High-frequency spontaneous mutation in the bacterio-opsin gene in Halobacterium halobium is mediated by transposable elements. Proc Natl Acad Sci USA 80:2201–2205
Davies RW, Gronenborn AM (1982) Molecular cloning of the gene for dihydrofolate reductase from Lactobacillus casei. Gene 17:229–233
Davis RW, Simon M, Davidson N (1971) Electron microscope heteroduplex methods for mapping regions of base sequence homology in nucleic acids. Methods Enzymol 31:413–428
Engler JA, van Bree MP (1981) The nucleotide sequence and protein-coding capability of the transposable element IS5. Gene 14:155–163
Ghosal D, Sommer H, Saedler H (1979) Nucleotide sequence of the transposable DNA-element IS2. Nucleic Acids Res 6:1111–1122
Gold L, Pribnow D, Schneider T, Shinedling S, Singer BS, Stormo G (1981) Translational initiation in prokaryotes. Annu Rev Microbiol 35:365–403
Grindley NDF, Joyce CM (1980) Genetic and DNA sequence analysis of the kanamycin resistance transposon Tn903. Proc Natl Acad Sci USA 77:7176–7180
Halling SM, Simons RW, Way JC, Walsh RB, Kleckner N (1982) DNA sequence organization of IS10-right of Tn10 and comparison with IS10-left. Proc Natl Acad Sci USA 79:2608–2612
Iida S, Meyer J, Arber W (1983) Prokaryotic IS Elements. In: Shapiro JA (ed) Mobile genetic elements. Academic Press, New York, pp 159–221
Klaer R, Kühn S, Tillmann E, Fritz H-J, Starlinger P (1981) The sequence of IS4. Mol Gen Genet 181:169–175
Kleckner N (1981) Transposable elements in prokaryotes. Annu Rev Genet 15:341–404
Lee L-J, Hansen JB, Jagusztyn-Krynicka EK, Chassy BM (1982) Cloning and expression of the β-d-phosphogalactoside galactohydrolase gene of Lactobacillus casei in Escherichia coli K-12. J Bacteriol 152:1138–1146
Maizel Jr JV, Lenk RP (1981) Enhanced graphic matrix analysis of nucleic acid and protein sequences. Proc Natl Acad Sci USA 78:7665–7669
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, New York
Maxam AM, Gilbert W (1980) Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol 65:499–560
Mollet B, Iida S, Shepherd J, Arber W (1983) Nucleotide sequence of IS26, a new prokaryotic mobile genetic element. Nucleic Acids Res 11:6319–6330
Ohtsubo H, Ohtsubo E (1978) Nucleotide sequence of an insertion element, IS1. Proc Natl Acad Sci USA 75:615–619
Oka A, Sugisaki H, Takanami M (1981) Nucleotide sequence of the kanamycin resistance transposon Tn903. J Mol Biol 147:217–226
Rosenberg M, Court D (1979) Regulatory sequences involved in the promotion and termination of RNA transcription. Annu Rev Genet 13:319–353
Sako T (1985) Overproduction of staphylokinase in Escherichia coli and its characterization. Eur J Biochem, in press
Schoner B, Kahn M (1981) The nucleotide sequence of IS5 from Escherichia coli. Gene 14:165–174
Shapiro JA (ed) (1983) Mobile genetic elements. Academic Press, New York
Shimizu-Kadota M, Sakurai T (1982) Prophage curing in Lactobacillus casei by isolation of a thermoinducible mutant. Appl Environ Microbiol 43:1284–1287
Shimizu-Kadota M, Tsuchida N (1984) Physical mapping of the virion and the prophage DNAs of a temperate Lactobacillus phage ϕFSW. J Gen Microbiol 130:423–430
Shimizu-Kadota M, Sakurai T, Tsuchida N (1983) Prophage origin of a virulent phage appearing on fermentations of Lactobacillus casei S-1. Appl Environ Microbiol 45:669–674
Shimizu-Kadota M, Kiwaki M, Hirokawa H, Tsuchida N (1984) A temperate Lactobacillus phage converts into virulent phage possibly by transposition of the host sequence. Dev Ind Microbiol 25:151–159
Simsek M, DasSarma S, RajBhandary UL, Khorona HG (1982) A transposable element from Halobacterium halobium which inactivates the bacteriorhodopsin gene. Proc Natl Acad Sci USA 79:7268–7272
Trieu-Cuot P, Courvalin P (1984) Nucleotide sequence of the transposable element IS15. Gene 30:113–120
Williams SA, Hodges RA, Strike TL, Snow R, Kunkee RE (1984) Cloning the gene for the malolactic fermentation of wine from Lactobacillus delbrueckii in Escherichia coli and yeasts. Appl Environ Microbiol 47:288–293
Xu W-L, Doolittle WF (1983) Structure of the archaebacterial transposable element ISH50. Nucleic Acids Res 11:4195–4199
Yousaf SI, Carroll AR, Clarke BE (1984) A new and improved method for 3′-end labelling DNA using [α-32P]ddATP. Gene 27:309–313
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Shimizu-Kadota, M., Kiwaki, M., Hirokawa, H. et al. ISL1: a new transposable element in Lactobacillus casei . Molec. Gen. Genet. 200, 193–198 (1985). https://doi.org/10.1007/BF00425423
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DOI: https://doi.org/10.1007/BF00425423