Abstract
The morphological, cultural, and physiological characteristics are described of a yeast, LI70, which uses methanol as its source of energy and carbon; these characteristics have made it possible to identify the strain as Candida boidinii Ramirez. The identification was confirmed by a DNA-DNA genetic homology of 99.43% with the type strain of C. boidinii. Strain LI70 is not pathogenic.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Asthana, H., Humphrey, A. E. and Moritz, V. 1971. Growth of yeast on methanol as the sole carbon substrate. — Biotechnol. Bioeng. 13: 923–929.
Barnett, J. A. 1968. Biochemical differentiation of taxa with special reference to the yeasts. A discussion of the biochemical basis of the nutritional tests used for classification, p. 557–595. In G. C. Ainsworth and A. S. Sussman, (eds.), The Fungi, Vol. 3. — Academic Press, London.
Barnett, J. A. and Pankhurst, R. J. 1974. A new key to the yeasts. A key for identifying yeasts based on physiological tests only. — North-Holland Publ. Co., Amsterdam.
Bicknell, J. N. and Douglas, H. C. 1970. Nucleic acid homologies among species of Saccharomyces. — J. Bacteriol. 101: 505–512.
Campbell, I. 1974. Methods of numerical taxonomy for various genera of yeasts. — Adv. Appl. Microbiol. 17: 135–156.
Cardini, G., Cavalli, L. e Calcagno, B. 1976. Caratteristiche di un lievito coltivato su metanolo. Composizione degli acidi grassi. — Chim. Ind. (Milan), in press.
Cardini, G., Di Fiore, L. e Zotti, A. 1975. Caratteristiche di un lievito coltivato su metanolo. — Chim. Ind. (Milan) 57: 381–385.
Ciferri, R. 1958. Manuale di Micologia medica, I. — Cortina, Pavia.
Cooney, C. L., Makiguchi, N. and Montgomery, M. 1975. Effect of temperature and growth rate on viability and cell yield on methanol grown Hansenula polymorpha. — Abstr. Annu. Meet. Am. Soc. Microbiol. N.Y. 27 Apr.–2 May.
De Ley, J. and Tijtgat, R. 1970. Evaluation of membrane filter methods for DNA-DNA hybridization. — Antonie van Leeuwenhoek 36: 461–474.
Denhardt, D. T. 1966. A membrane-filter technique for the detection of complementary DNA. — Biochem. Biophys. Res. Comm. 23: 641–646.
Fryer, T. F., Reiter, B. and Lawrence, R. C. 1967. Lipolytic activity of lactic acid bacteria. — J. Dairy Sci. 50: 388–389.
Grillot, R. 1974. Contribution à l'identification des levures du Candida. Étude du métabolisme des glucides par une méthode normalisée. — Thèse d'Université de Pharmacie, Grenoble.
Grillot, R., Buissière, J., Doncet, M. et Ambroise-Thomas, P. 1973. Étude qualitative et cinétique du métabolisme des glucides chez les levures du genre Candida par une méthode normalisée. Résultats préliminaires. — C.R. 9th Congr. Int. Med. Trop. Malaria, Athènes 2: 47–48.
Grinbergs, L. and Yarrow, D. 1970. Two new Candida species: Candida chilensis sp.n and Candida valdiviana sp.n. — Antonie van Leeuwenhoek 36: 143–148.
Guicciardi, A., Biffi, M. R., Manachini, P. L., Craveri, A., Scolastico, C., Rindone, R. e Craveri, R. 1968. Ricerche preliminari su un nuovo schizomicete termofilo del genere Bacillus e caratterizzazione del pigmento rosso prodotto. — Ann. Micr. 18: 191–205.
Hazeu, W., de Bruyn, J. C. and Bos, P. 1972. Methonol assimilation by yeasts. — Arch. Mikrobiol. 87: 185–188.
Hedrick, L. R. and Dupont, P. D. 1968. The utilization of l-amino acids as carbon source by yeasts of the genera Hansenula and Trichosporon. — Antonie van Leeuwenhoek 34: 465–473.
Henninger, W. und Windisch, S. 1975. Pichia lindnerii sp.n., eine neue Methanol assimilierende Hefe aus Erde. — Arch. Microbiol. 105: 47–48.
Kato, K., Kurimura, Y., Makiguchi, N. and Asai, Y. 1974. Determination of methanol strongly assimilating yeasts. — J. Gen. Appl. Microbiol. 20: 123–127.
Levine, D. W. and Cooney, C. L. 1973. Isolation and characterization of a thermotolerant methanol-utilizing yeast. — Appl. Microbiol. 26: 982–990.
Lodder, J. 1971. The Yeasts. A taxonomic study. — North-Holland Publ. Co., Amsterdam.
Martini, A., Phaff, H. J. and Douglass, S. A. 1972. Deoxyribonucleic acid base composition of species in the yeast genus Kluyveromyces van der Walt emend. van der Walt. —J. Bacteriol. 111: 481–487.
Ogata, K., Nishikawa, H., Oshugi, M. and Tochikura, T. 1970. Studies on the production of yeast. I. A yeast utilizing methanol as a sole carbon source. — J. Ferment. Technol. 48: 389–396.
Oki, T., Kouno, K., Kitai, A. and Ozaki, A. 1972. New yeasts capable of assimilating methanol. — J. Gen. Appl. Microbiol. 18: 295–305.
Ramirez, C. 1953. Estudio sobre nuevas especies de levaduras aisladas de diferentes sustratos. — Microbiol. Esp. 6: 249–253.
Sahm, H. und Wagner, F. 1972. Mikrobielle Verwertung von Methanol. Isolierung und Charakterisierung der Hefe Candida boidinii. — Arch. Mikrobiol. 84: 29–42.
Santa Maria, J. 1958. Ecologia de las levaduras. I. Nuevas especies aisladas de alpechin. —Bol. Inst. Nac. Invest. Agron. (Spain) 38: 301–313.
Scheda, R. and Yarrow, D. 1966. The instability of physiological properties used as criteria in the taxonomy of yeasts. — Arch. Mikrobiol. 55: 209–225.
Segretain, C., Drouhet, E. et Mariat, F. 1974. Diagnostic de laboratoire en mycologie médicale. — Maloine S. A. Ed., Paris.
Skinner, C. H. and Fletcher, D. W. 1960. A review of the genus Candida. — Bacteriol. Rev. 24: 397–416.
Tezuka, H., Nakahara, T., Minoda, Y. and Yamada, R. 1975. Production of yeast cells from methanol. — Agric. Biol. Chem. 39: 285–286.
Volfová, O. and Pilát, P. 1974. Studies on methanol-oxidizing yeasts. I. Isolation and growth studies. — Folia Microbiol. 19: 249–256.
van der Walt, J. P. 1971. Criteria and methods used in classification. — In J. Lodder, (ed.), The Yeasts. — North-Holland Publ. Co., Amsterdam.
Yokotsuka, I. and Goto, S. 1955. Studies on Japanese wine film yeasts. Part 4. On new species of genus Candida and Trichosporon. — J. Agric. Chem. Soc. Japan 29: 132–135.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Craveri, R., Cavazzoni, V., Sarra, P.G. et al. Taxonomical examination and characterization of a methanol-utilizing yeast. Antonie van Leeuwenhoek 42, 533–540 (1976). https://doi.org/10.1007/BF00410184
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00410184