Abstract
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1.
The inhibitory effects of CPTA, nicotine, DPA, and San 6706 on carotenogenesis in Myxococcus fulvus were investigated.
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2.
The effects of CPTA, D-nicotine, and L-nicotine were very similar. The action of the drugs wasadditive. The cyclization was inhibited at low doses, the introduction of the hydroxyl group at C-1′ at higher doses. Lycopene accumulated at high drug concentration. The mode of action of the inhibitors is discussed.
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3.
In a carotenoid mutant of M. fulvus a stimulation of the “7,8-dehydrogenase” by CPTA was observed.
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4.
The specific carotenoid content of bacteria was increased by DPA due to an enhanced formation of phytoene. At low doses of DPA small amounts of an intermediate carotenoid glucoside ester, a 7,8-dihydro derivative, were detected.
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5.
DPA was taken up by the plasma membrane. Quantitative removal of DPA by washing was not possible.
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6.
San 6706 specifically and reversibly blocked the desaturation of phytoene.
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Abbreviations
- CPTA:
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2-(4-chlorophenylthio) triethylamine hydrochloride
- DPA:
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diphenylamine
- San 6706:
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4-chloro-5-(dimethylamino)-2-α,α,α(trifluoro-m-tolyl(-3(2H)-pyridazinone
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Kleinig, H. Inhibition of carotenoid synthesis in Myxococcus fulvus (Myxobacterales) . Arch. Microbiol. 97, 217–226 (1974). https://doi.org/10.1007/BF00403061
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DOI: https://doi.org/10.1007/BF00403061