Abstract
The photosynthetic unit of Rhodopseudomonas viridis contains a reaction centre (P960) and a light harvesting complex (B1015). Immune electron microscopy combined with image processing has allowed the central core of the photosynthetic unit to be identified as the reaction centre and the surrounding protein ring as the light harvesting complex. This light harvesting complex, subdivided into twelve subunits was shown to contain 24 bacteriochlorophyll b molecules. A model is presented which may account for the far red shift of the Qy absorption of the bacteriochlorophyll b molecules in vivo.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Aebi U, Ten Heggeler B, Onorato L, Kistler J, Showe MK (1977) New method for localizing proteins in periodic structures: Fab fragments labeling combined with image processing of electron micrographs. Proc Natl Acad Sci USA 74:5514–5518
Breton J, Farkas DL, Parson WW (1985) Organization of the antenna bacteriochlorophylls around the reaction center of Rhodopseudomonas viridis investigated by photoselection techniques. Biochim Biophys Acta 808:421–427
Brunisholz RA, Jay FA, Suter F, Zuber H (1985) The light harvesting polypeptides of Rhodopseudomonas viridis. The complete amino acid sequence of B1015-α, B1015-β and B1015-γ. Biol Chem Hoppe-Seyler 366:87–98
Cantor CR, Schimmel PR (1980) Techniques for the study of biological structure and function. In: Bartlett AC, Vapuek PC, McCombs LW (eds) Biophysical Chemistry, Part II, W. H. Freeman and Company, San Francisco, pp 390–408
Cogdell RJ, Zuber H, Thornber JP, Drews G, Gingras G, Niederman RA, Parson WW, Feher G (1985) Recommendations for the naming of photochemical reaction centres and light harvesting pigment-protein complexes from purple photosynthetic bacteria. Biochim Biophys Acta 806:185–186
Deisenhofer J, Epp O, Miki K, Huber R, Michel H (1984) X-ray structure analysis of a membrane protein complex. Electron density map at 3 Å resolution and a model of the chromophores of the photosynthetic reaction center from Rhodopseudomonas viridis. J Mol Biol 180:385–398
Deisenhofer J, Michel H, Huber R (1985) The structural basis of photosynthetic light reactions in bacteria. TIBS 10:243–248
Deisenhofer J, Epp O, Miki HK, Huber R, Michel H (1985b) Structure of the protein subunits in the photosynthetic reaction centre of Rhodopseudomonas viridis at 3 Å resolution. Nature 318:618–624
Engelhardt H, Baumeister W, Saxton WO (1983) Electron microscopy of photosynthetic membranes containing bacteriochlorophyll-b. Arch Microbiol 135:169–175
Garcia A, Vernon LP, Ke B, Mollenhauer H (1968) Some structural and photochemical properties of Rhodopseudomonas species NHTC 133 subchromatophore particles obtained by treatment with Triton X-100. Biochem 7:326–332
Henderson R (1975) The structure of the purple membrane from Halobacterium halobium: analysis of the X-ray diffraction pattern. J Mol Biol 93:123–138
Henderson R, Unwin PNT (1975) Three dimensional model of purple membrane obtained by electron microscopy. Nature 257:28–32
Jay FA, Lambillotte M (1985) Isolation of orientated vesicles from thylakoids of Rhodopseudomonas viridis and topographical studies. Eur J Cell Biol 37:7–13
Jay F, Lambillotte M, Muehlethaler K (1983) Localisation of Rhodopseudomonas viridis reaction centre and light harvesting proteins using ferritin antibody labelling. Eur J of Cell Biol 30:1–8
Jay FA, Lambillotte M, Stark W, Muehlethaler K (1984) The preparation and characterisation of native photoreceptor units from the thylakoids of Rhodopseudomonas viridis. EMBO J 3:773–776
Jay FA, Lambillotte M, Wyss F (1985) Immune electron microscopy with monoclonal antibodies against Rhodopseudomonas viridis thylakoid polypeptides. Eur J Cell Biol 37:14–20
Jones OTG, Saunders VA (1972) Energy-linked electron transfer reactions in Rhodopseudomonas viridis. Biochim Biophys Acta 275:427–436
Kramer HJM, Van Grondelle R, Hunter CR, Westerhuis WHJ, Amesz J (1984) Pigment organisation of the B800-850 antenna complex of Rhodopseudomonas sphaeroides. Biochim Biophys Acta 765:156–165
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin-phenol-reagent. J Biol Chem 193:265–275
Ludwig FR, Jay FA (1985) Reversible chemical cross-linking of the light-harvesting polypeptides of Rhodopseudomonas viridis. Eur J Biochem 151:83–87
Michel H, Deisenhofer J (1986) X-ray diffraction studies on a crystalline bacterial photosynthetic reaction centre: A progress report and conclusions on the structure of photosystem II reaction centres. In: Staehelin LA, Arntzen CJ (eds) Encyclopedia of Plant Physiology, Photosynthesis III. Springer, Berlin Heidelberg New York
Michel H, Deisenhofer J, Miki K, Weyer K, Lottspeich F (1983) Crystallization of membrane proteins and actual state of structure analysis of photosynthetic reaction centre crystals. In: Quagliariello E, Palmieri F (eds) Structure and function of membrane proteins. Elseviers Sci Publ, pp 191–197
Nabedryk E, Berger G, Andrianambinintsoa S, Breton J (1985) Comparison of α-helix orientation in chromatophore, quantasome and reaction centre of Rhodopseudomonas viridis by circular dichroism and polarized infrared spectroscopy. Biochim Biophys Acta 809:271–276
Paillotin G, Vermeglio A, Breton J (1979) Orientation of reaction center and antenna chromophores in the photosynthetic membrane of Rhodopseudomonas viridis. Biochim Biophys Acta 545:249–264
Peters J, Welte W, Drews G (1984) Topographical relationships of polypeptides in the photosynthetic membrane of Rhodopseudomonas viridis investigated by reversible chemical cross-linking. FEBS Lett 171:267–270
Stark W, Kuehlbrandt W, Wildhaber I, Wehrli E, Muehlethaler K (1984) The structure of the photoreceptor unit of Rhodopseudomonas viridis. EMBO J 3:777–783
Theiler R, Suter F, Wiemken V, Zuber H (1984) The light-harvesting polypeptides of Rhodopseudomonas sphaeroides R-26.1. I. Isolation, purification and sequence analysis. Hoppe-Seyler's Z Physiol Chem 365:703–719
Thornber JP (1971) The photochemical reaction centre of Rhodopseudomonas viridis. In: San Pietro A, Colowick SP, Kaplan NO (eds) Methods in Enzymology 23:688–691, Academic Press, New York
Thornber JP, Cogdell RJ, Seftor REB, Webster GD (1980) Further studies on the composition and spectral properties of the photochemical reaction centres of bacteriochlorophyll b-containing bacteria. Biochim Biophys Acta 593:60–75
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Stark, W., Jay, F. & Muehlethaler, K. Localisation of reaction centre and light harvesting complexes in the photosynthetic unit of Rhodopseudomonas viridis . Arch. Microbiol. 146, 130–133 (1986). https://doi.org/10.1007/BF00402339
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00402339