Abstract
Twenty known gibberellins (GAs) have been identified by combined capillary gas chromatography-mass spectrometry in extracts from less than 10 g fresh weight of maturing seeds of the cucurbit Sechium edule Sw. The GAs are predominantly 3- and-or 13-hydroxylated. This is the first reported identification of non-conjugated 13-hydroxylated GAs in a cucurbit. Gibberellin A8 and gibberellin A8-catabolite are the major GAs in terms of quantity and are largely accumulated in the testa. The catabolites of 2β-hydroxylated GAs are α,β-unsaturated ketones which no longer possess of a γ-lactone. They were hitherto known only in legumes. The presence of GA8-catabolite as a major component of Sechium seeds indicates that the distribution of these GA-catabolites may be more widespread than previously envisaged. The localization of known GAs in maturing seeds of the legume Phaseolus coccineus L. was found to resemble closely that in Sechium. Gibberellin A8, a putative conjugate of GA8 and GA8-catabolite are accumulated in the testa. The localization in the testa of end-products of the GA-biosynthetic pathway, which was first observed in maturing seeds of Pisum sativum, and is now described in Phaseolus and Sechium, may be a general feature of seed development.
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Abbreviations
- GAn :
-
gibberellin An
- GC-MS:
-
combined gas chromatography-mass spectrometry
References
Beeley, L.J., Gaskin, P., MacMillan, J. (1975) Gibberellin A43, and other terpenes in endosperm of Echinocystis macrocarpa. Phytochemistry 14, 779–783
Blechschmidt, S., Castel, U., Gaskin, P., Hedden, P., Graebe, J.E., MacMillan, J. (1984) GC/MS analysis of the plant hormones in seeds of Cucurbita maxima. Phytochemistry 23, 553–558
Ceccarelli, N., Lorenzi, R. (1982) Biosynthesis of GA9 in a cell-free system of Sechium edule S. Z. Pflanzenphysiol. 108, 477–479
Ceccarelli, N., Lorenzi, R. (1983) Gibberellin biosynthesis in endosperm and cotyledons of Sechium edule seeds. Phytochemistry 22, 2203–2206
Durley, R.C., MacMillan, J., Pryce, R.J. (1971) Investigation of gibberellins and other growth substances in the seed of Phaseolus multiflorus and of Phaseolus vulgaris by gas chromatography and by gas chromatography-mass spectrometry. Phytochemistry 10, 1891–1908
Durley, R.C., Sassa, T., Pharis, R.P. (1979) Metabolism of tritiated gibberellin A20 in immature seeds of dwarf pea cv. Meteor. Plant Physiol. 64, 214–219
Elson, G.W., Jones, D.F., MacMillan, J., Suter, P.J. (1964) Plant hormones. IV. Identification of the gibberellins of Echinocystis macrocarpa Greene by thin layer chromatography. Phytochemistry 3, 93–101
Fukui, H., Koshimizu, K., Nemori, R. (1978) Two new gibberellins A50 and A52 in seeds of Lagenaria leucantha. Agric. Biol. Chem. 42, 1571–1576
Fukui, H., Koshimizu, K., Usuda, S., Yamazuki, Y. (1977a) Isolation of plant growth regulators from seeds of Cucurbita pepo L. Agric. Biol. Chem. 41, 175–180
Fukui, H., Nemori, R., Koshimizu, K., Yamazuki, Y. (1977b) Structures of gibberellins A39, A48, A49 and a new kaurenolide in Cucurbita pepo L. Agric. Biol. Chem. 41, 181–187
Gaskin, P., Gilmour, S.J., Lenton, J.R., MacMillan, J., Sponsel, V.M. (1984) Endogenous gibberellins and kauranoids identified from developing and germinating barley grain. J. Plant Growth Regul. 2, 229–242
Gaskin, P., Kirkwood, P.S., MacMillan, J. (1981) Partial synthesis of ent-13-hydroxy-2-oxo-20-norgibberella-1(10), 16-diene-7,19-doic acid, a catabolite of GA29, and of related compounds. J. Chem. Soc. Perkin Trans. 1, 1083–1091
Gaskin, P., MacMillan, J., Firn, R.D., Pryce, R.J. (1971) ‘Parafilm’ a convenient source of n-alkane standards for the determination of gas chromatographic retention indices. Phytochemistry 10, 1155–1157
Hemphill, D.D., Baker, L.R., Sell, H.M. (1972) Isolation and identification of the gibberellins of Cucumis sativus and Cucumis melo. Planta 103, 241–248
Hemphill, D.D., Baker, L.R., Sell, H.M. (1973) Isolation of novel conjugated gibberellins from Cucumis sativus seed. Can. J. Biochem. 51, 1647–1653
Hutchinson, J. (1967) The genera of flowering plants. Clarendon Press, Oxford
Lorenzi, R., Ceccarelli, N. (1983) Endogenous gibberellins in endosperms and cotyledons of Sechium edule during seed growth and maturation. Phytochemistry 22, 2189–2192
MacMillan, J., Suter, P.J. (1958) The occurrence of gibberellin A1 in higher plants: isolation from the seed of runner bean (Phaseolus multiflorus). Naturwissenschaften 45, 46
Sponsel, V.M. (1980) Gibberellin metabolism in legume seeds. In: Gibberellins — chemistry, physiology and use. British Plant Growth Regulator Group, Monograph No. 5, pp. 49–62, Lenton, J.R., ed. British Plant Growth Regulator Group, Wantage
Sponsel, V.M. (1983a) The localization, metabolism and biological activity of gibberellins in maturing and germinating seeds of Pisum sativum cv. Progress No. 9. Planta 159, 454–468
Sponsel, V.M. (1983b) In vivo gibberellin metabolism in higher plants. In: The biochemistry of gibberellins, vol. 1, pp. 151–250, Crozier, A., ed. Praeger, New York
Sponsel, V.M., Gaskin, P., MacMillan, J. (1979) The identification of gibberellins in immature seeds of Vicia faba, and some chemotaxonomic considerations. Planta 146, 101–105
Sponsel (née Frydman) V.M., MacMillan, J. (1977) Further studies on the metabolism of gibberellins (GAs) A9, A20 and A29 in immature seeds of Pisum sativum cv. Progress No. 9. Planta 135, 129–136
Sponsel, V.M., MacMillan, J. (1978) Metabolism of gibberellin A29 in seeds of Pisum sativum cv. Progress No. 9; use of [2H] and [3H]GAs, and the identification of a new GA catabolite. Planta 144, 69–78
Sponsel, V.M., MacMillan, J. (1980) Metabolism of [13C1]gibberellin A29 to [13C1]gibberellin catabolite in maturing seeds of Pisum sativum cv. Progress No. 9. Planta 150, 46–52
Whitaker, T.W., Davis, G.N. (1962) Cucurbits botany, cultivation and utilization. Interscience, New York
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Albone, K.S., Gaskin, P., MacMillan, J. et al. Identification and localization of gibberellins in maturing seeds of the cucurbit Sechium edule, and a comparison between this cucurbit and the legume Phaseolus coccineus . Planta 162, 560–565 (1984). https://doi.org/10.1007/BF00399923
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DOI: https://doi.org/10.1007/BF00399923