Abstract
The reversibility of the inhibition of photosynthetic reactions by water stress was examined with four systems of increasing complexity—stromal enzymes, intact chloroplasts, mesophyll protoplasts, and leaf slices. The inhibition of soluble chloroplast enzymes by high solute concentrations was instantly relieved when solutes were properly diluted. In contrast, photosynthesis was not restored but actually more inhibited when isolated chloroplasts exposed to hypertonic stress were transferred to conditions optimal for photosynthesis of unstressed chloroplasts. Upon transfer, chloroplast volumes increased beyond the volumes of unstressed chloroplasts, and partial envelope rupture occurred. In protoplasts and leaf slices, considerable and rapid, but incomplete restoration of photosynthesis was observed during transfer from hypertonic to isotonic conditions. Chloroplast envelopes did not rupture in situ during water uptake. It is concluded that inhibition of photosynthesis by severe water stress is at the biochemical level brought about in part by reversible inhibition of chloroplast enzymes and in part by membrane damage which requires repair mechanisms for reversibility. Both soluble enzymes and membranes appear to be affected by the increased concentration of internal solutes, which is caused by dehydration.
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Arnon, D.I. (1949) Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris. Plant Physiol. 24, 1–15
Ashton, F.M. (1956) Effects of a series of cycles of alternating low and high soil water contents on the rate of apparent photosynthesis in sugar cane. Plant Physiol. 31, 226–274
Boyer, J.S. (1971) Recovery of photosynthesis in sunflower after a period of low leaf water potential. Plant Physiol. 47, 816–820
Cockburn, W., Walker, D.A., Baldry, C.W. (1968) The isolation of spinach chloroplasts in pyrophosphate media. Plant Physiol. 43, 1415–1418
Edwards, G.E., Robinson, S.P., Tyler, N.J.C., Walker, D.A. (1978) Photosynthesis by isolated protoplasts, protoplast extracts, and chloroplasts of wheat. Plant Physiol. 62, 313–319
Fischer, R.A., Hsiao, T.C., Hagan, R.M. (1970) After-effect of water stress on stomatal opening potential. I. Techniques and magnitude. J. Exp. Bot. 21, 371–385
Heber, U., Santarius, K.A. (1970) Direct and indirect transfer of ATP and ADP across the chloroplast envelope. Z. Naturforsch. 25b, 718–728
Heldt, H.W. (1979) Light-dependent changes of stomatal H+ and Mg2+ concentrations controlling CO2 fixation, In: Encyclopedia of plant physiol, vol. 6, pp. 202–207, Gibbs, M., Latzko, E., eds. Springer, Berlin Heidelberg New York
Kaiser, W.M., Heber U. (1981) Photosynthesis of isolated chloroplasts and protoplasts under osmotic stress. Effects of high solute concentrations on the permeability properties of the chloroplast envelope and on activity of stroma enzymes. Planta 153, 423–429
Kaiser, W.M., Stepper, W., Urbach, W. (1981a) Photosynthesis of isolated chloroplasts and protoplasts under osmotic stress. Reversible swelling of chloroplasts by hypotonic treatment and its effect on photosynthesis. Planta 151, 375–380
Kaiser, W.M., Kaiser, G., Prachuab, P.K., Wildman, S.G., Heber, U. (1981b) Photosynthesis of isolated chloroplasts and protoplasts under osmotic stress. Inhibition of photosynthesis of intact chloroplasts, protoplasts and leaf slices at low water potentials. Planta 153, 416–422
Kramer, P.J. (1950) Effects of wilting on the subsequent intake of water by plants. Am. J. Bot. 37, 280–284
Plaut, Z. (1971) Inhibition of photosynthetic carbon dioxide fixation in isolated spinach chloroplasts exposed to reduced osmotic potentials. Plant Physiol. 48, 591–595
Santarius, K.A. (1967) Das Verhalten von CO2-Assimilation, NADP-und PGS-Reduktion und ATP-Synthese intakter Blattzellen in Abhängigkeit vom Wassergehalt. Planta 73, 228–242
Troughton, J.H. (1969) Plant water status and carbon dioxide exchange of cotton leaves. Aust. J Biol. Sci. 22, 289–302
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Kaiser, W.M., Kaiser, G., Schöner, S. et al. Photosynthesis under osmotic stress. Planta 153, 430–435 (1981). https://doi.org/10.1007/BF00394981
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DOI: https://doi.org/10.1007/BF00394981