Abstract
This study reports the use of lactate dehydrogenase release to monitor changes in culture viability in flask culture and fixed bed, porosphere bioreactor systems. Lactate dehydrogenase release shows good agreement with increase in non-viable cell numbers and decline in glucose utilisation in flask cultures. Studies with the immobilised system show that lactate dehydrogenase release can detect loss of viability which is not always indicated by a decrease in glucose utilisation. The data show that culture viability in a repeated-feed-and-harvest system is influenced markedly by both a) the medium change regime itself and b) the use of an immobilised bioreactor compared to a flask system for the same medium change regime.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Clarke JB and Spier RE (1980) Variation in the susceptibility of BHK populations and cloned cell lines to three strains of FMD virus. Arch. Virol. 86: 1–9.
Cook JA and Mitchell JB (1989) Viability measurements in mammalian cell systems. Anal. Biochem. 179: 1–7.
Donnelly M and Scheffler IE (1976) Energy metabolism in respiration-deficient and wild type chinese hamster fibroblasts in culture. J. Cell Physiol. 89: 39–52.
Griffiths JB (1988) Overview of cell culture systems and their scale-up. Animal Cell Biotechnol. 3: 179–220.
Looby P and Griffiths JB (1988a) Immobilisation of animal cells in fixed and fluidised porous glass sphere reactors. In: Spier RE, Griffiths JB, Stephenne J and Crooy PJ (eds.) Advances in Animal Cell Biology and Technology for Bioprocesses. Butterworths, Guildford, pp. 336–344.
Looby D and Griffiths JB (1988b) Fixed bed porous glass sphere (porosphere) bioreactors for animal cells. Cytotechnology 1: 339–346.
Petersen JF, McIntire LV and Papoutsakis ET (1988) Shear sensitivity of cultured hybridoma cells (CRL-8018) depends on mode of growth, culture age and metabolite concentration. J. Biotechnol. 7: 229–246.
Reuveny S, Velez D, Macmillan JD and Miller L (1986) Factors affecting cell growth and monoclonal antibody production in stirred reactors. J. Immunol. Meth. 86: 53–59.
Vassault A (1983) Lactate dehydrogenase. UV-method with pyruvate and NADH. In: Bergmeyer HU, Bergmeyer J and Grassl M (eds.) Methods of Enzymatic Analysis. III. Enzymes 1: Oxidoreductases, Transferases, 3rd ed. Verlag-Chemie, Weinheim, pp. 118–126.
Wright JP and Balfor AH (1983) Monoclonal antibodies to Toxoplasma gondii. Parasitol. 87: LXVI.
Zielke HR, Ozand PT, Tildon JT, Seudalion DA and Cornblath M (1976) Reciprocal regulation of glucose and glutamine utilisation by cultured human diploid fibroblasts. J. Cell Physiol. 95: 41–48.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Racher, A.J., Looby, D. & Griffiths, J.B. Use of lactate dehydrogenase release to assess changes in culture viability. Cytotechnology 3, 301–307 (1990). https://doi.org/10.1007/BF00365494
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00365494