Summary
Mutations conferring resistance to arginine hydroxamate in Bacillus subtilis 168 have been located on the genetic map by PBSl-mediated transduction. The majority of these mutations, belonging to classes 1, 2 and 4 of Harwood and Baumberg (1977) and affecting only expression of arginine catabolic enzymes, map at a locus designated ahrA cotransducible with cysA, purA and sacA. The order of markers in this region appears to be sacA-ahrA-purA-cysA. Certain anomalies were observed in the properties of Pur+ transductants from crosses with an Ahr donor and a purA recipient. A single ahr mutation (class 3), also affecting only arginine catabolism, maps between ctrA and sacA at a locus designated ahrB. Two others (class 6), affecting simultaneously enzymes of both arginine biosynthesis and catabolism, map between lys and aroD at a locus designated ahrC. Preliminary attempts to define the nature of functional products specified by these ahr loci suggest that a protein is encoded at ahrA.
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Abbreviations
- OAT:
-
ornithine aminotransferase
- OCT:
-
ornithine carbamoyltransferase
- AS:
-
argininosuccinase
- AH:
-
arginine hydroxamate
- Ahs, Ahr:
-
arginine hydroxamate-sensitive or-resistant
- Ahos, Ahor:
-
sensitive or resistant to arginine hydroxamate in the presence of ornithine
- ahr :
-
allele conferring arginine hydroxamate resistance
- Out+, Out- :
-
able or unable to utilise ornithine as sole nitrogen source
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Communicated by B.A. Bridges
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Mountain, A., Baumberg, S. Map locations of some mutations conferring resistance to arginine hydroxamate in Bacillus subtilis 168. Molec. gen. Genet. 178, 691–701 (1980). https://doi.org/10.1007/BF00337880
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DOI: https://doi.org/10.1007/BF00337880