Abstract
The replication patterns of larval salivary gland chromosomes of D. hydei and D. melanogaster were studied by autoradiography with tritiated thymidine injected in mid third instar larvae. The male X chromosome showed a different replication behavior in comparison to that of the female X chromosome and autosomes. It is concluded that the male X chromosome finishes its replication earlier than the female X chromosome. Moreover, the time needed for a complete replication cycle of individual identical replication units was found to be shorter in the male than in the female X chromosome. Although the whole X chromosomes behave different there were no differences observed in the sequence of the discontinuous labeling patterns of the two types of X chromosome. One autosomal replication unit was observed which showed a different replication behavior in males and females. The possible origin of the differential behavior of the two X chromosomes is discussed in terms of their difference in degree of polyteny.
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Literature
Aronson, J. F., G. T. Rudkin, and J. Schultz: A comparison of giant X chromosomes in male and female Drosophila melanogaster by cytophotometry in the ultraviolet. J. Histochem. Cytochem. 2, 458–459 (1954).
Baer, D.: Asynchronous replication of DNA in a heterochromatic set of chromosomes in Pseudococcus obscurus. Genetics 52, 275–285 (1965).
Berendes, H. D.: The salivary gland chromosomes of Drosophila hydei Sturtevant. Chromosoma (Berl.) 14, 195–206 (1963a).
—: Asynapsis in the salivary gland chromosomes of Drosophila hydei. Genetica 33, 288–300 (1963b).
Evans, H. J.: Uptake of H3thymidine and patterns of DNA replication in nuclei and chromosomes of Vicia faba. Exp. Cell Res. 35, 381–393 (1964).
Gabrusewycz-Garcia, N.: Cytological and autoradiographic studies in Sciara coprophila salivary gland chromosomes. Chromosoma (Berl.) 15, 312–344 (1964).
Gay, H.: Chromosome structure and function. Yearbook Carnegie Inst. Wash. 62, 503–510 (1963).
Hsu, T. C., W. Schmid, and E. Stubblefield: DNA replication sequences in higher animals. In: The role of chromosomes in development (ed. M. Locke), p. 82–112. New York: Academic Press 1964.
Keyl, H.-G., and C. Pelling: Differentielle DNS-Replikation in den Speicheldrüsenchromosomen von Chironomus thummi. Chromosoma (Berl.) 14, 347–359 (1963).
Lima de Faria, A.: Differential uptake of tritiated thymidine into hetero- and euchromatin in Melanoplus and Secale. J. biophys. biochem. Cytol. 6, 457–466 (1959).
Morishima, A., M. M. Grumbach, and J. H. Taylor: Asynchronous duplication of human chromosomes and the origin of sex chromatin. Proc. nat. Acad. Sci. (Wash.) 48, 756–763 (1962).
Mukherjee, A. S., and W. Beermann: Synthesis of ribonucleic acid by the X chromosome of Drosophila melanogaster and the problem of dosage compensation. Nature (Lond.) 207, 785–786 (1965).
Muller, H. J.: Further studies on the nature and causes of gene mutations. Proc. 6th Int. Congr. Genet. 1, 312–255 (1932).
Nicklas, R. B., and R. A. Jaqua: X chromosome DNA replication: Developmental shift from synchrony to asynchrony. Science 147, 1041–1043 (1965).
Offermann, C. A.: Branched chromosomes as symmetrical duplications. J. Genet. 32, 103–116 (1936).
Pelling, C.: A replicative and synthetic chromosomal unit: The modern concept of the chromomere. Proc. roy. Soc. B 164, 279–289 (1966).
Plaut, W., and D. Nash: Localized DNA synthesis in polytene chromosomes and its implications. In: The role of chromosomes in development (ed. M. Locke), p. 113–135. New York: Academic Press 1964.
—, and F. Fanning: Ordered replication of DNA in polytene chromosomes of Drosophila melanogaster. J. molec. Biol. 16, 85–93 (1966).
Rodkiewicz, B., and M. J. Olszewska: Asynchronie intra-et interchromosomique de l'incorporation de la thymidine H3 dans les chromosomes d'Haemanthus katharinae Bak. Chromosoma (Berl.) 14, 568–571 (1963).
Rudkin, G. T.: The proteins of the polytene chromosomes. In: The nucleohistones (ed. J. Bonner and P. Ts'o). San Francisco: Holden Day Inc. 1964.
Schmid, W.: DNA replication patterns of human chromosomes. Cytogenetics 2, 86–110 (1963).
Stern, C.: Dosage compensation: Development of a concept and new facts. Canad. J. Genet. Cytol. 2, 105–118 (1960).
Taylor, J. H.: Asynchronous duplication of chromosomes in cultured cells of Chinese hamster. J. biophys. biochem. Cytol. 7, 455–463 (1960).
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Berendes, H.D. Differential replication of male and female X-chromosomes in Drosophila. Chromosoma 20, 32–43 (1966). https://doi.org/10.1007/BF00331896
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DOI: https://doi.org/10.1007/BF00331896