Summary
Previous tetrad analyses defined a yeast strain (332-7c) as containing a single nuclear gene (11.8 map units from the centromere) conferring resistance to oligomycin. Resistance to 18 additional inhibitors of mitochondrial function (Table 1) was determined on (i) ascospore isolates from tetrads segregating 2 resistant: 2 sensitive for oligomycin (Table 2) and (ii), spontaneously derived sensitive isolates of the oligomycin resistant strain (Tables 3 and 4). The observed pattern of resistance suggests that the gene for resistance to oligomycin also results in (i) cross resistance to rutamycin, venturicidin, triethyltin bromide, antimycin A, carbonylcyanide m-chlorophenylhydrazone, tetra-N-butylammonium bromide, dibenzyl-dimethylammonium chlorop and tetracycline and (ii), collateral sensitivity to paromomycin, neomycin, dequalinium chloride, ethidium bromide and acriflavin.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Aldridge, W. N., Street, B. W.: Oxidative phosphorylation. The relation between the specific binding of trimethyltin and triethyltin to mitochondria and their effects on various mitochondrial functions. Biochem. J. 124, 221–234 (1971)
Avner, P. R., Griffiths, D. E.: Studies on energy-linked reactions. Isolation and characterization of oligomycin-resistant of Saccharomyces cerevisiae. Europ. J. Biochem. 32, 301–311 (1973a)
Avner, P. R., Griffiths, D. E.: Studies on energy-linked reactions. Genetic analysis of oligomycin-resistant mutants of Saccharomyces cerevisiae. Europ. J. Biochem. 32, 312–321 (1973b)
Bakeeva, L. E., Grinius, L. L., Jasaitis, A. A., Kuliene, V. V., Levitsky, D. O., Liberman, E. A., Severina, I. I., Skulachev, V. P.: Conversion of biomebrane-produced energy into electric form. II. Intact mitochondria. Biochim. biophys. Acta (Amst.) 216, 13–21 (1970)
Bech-Hansen, N. T., Rank, H. G.: Ethidium bromide resistance and petite induction in Saccharomyces cerevisiae. Canad. J. Genet. Cytol. 14, 681–689 (1972)
Beck, J. C., Parker, J. H., Balcavage, W. X., Mattoon, J. R.: Mendelian genes affecting development and function of yeast mitochondria. Autonomy and biogenesis of mitochondria and chloroplasts, eds. N. K. Boardman, A. W. Linnane, and R. M. Smillie, p. 194–204. Amsterdam: North Holland 1971
Davey, P. J., Haslam, J. M., Linnane, A. W.: Biogenesis of motochondria. 12. The effects of aminoglycoside antibiotics on the mitochondrial and cytoplasmic protein-synthesizing systems of Saccharomyces cerevisiae. Arch. Biochem. Biophys. 139, 54–64 (1970)
Fukuhara, H., Kujawa, C.: Selective inhibition of the in vivo transcription of mitochondrial DNA by ethidium bromide and by acriflavin. Biochem. biophys. Res. Commun. 41, 1002–1008 (1970)
Goldring, E., Grossman, L., Krupnick, D., Cryer, D., Marmur, J.: The petite mutation in yeast. Loss of mitochondrial deoxyribonucleic acid during induction of petites with ethidium bromide. J. molec. Biol. 52, 323–335 (1970)
Grivell, L. A., Reijnders, L.: Altered mitochondrial ribosomes in a cytoplasmic mutant of yeast. FEBS Letters 16, 159–163 (1971)
Hackenbrock, C.: States of activity and structure in mitochondrial membranes. Ann. N.Y. Acad. Sci. 195, 492–505 (1972)
Heytler, P. G., Prichard, W. W.: A new class of uncoupling agents-carbonyl cyanide phenyl-hydrazones. Biochem. biophys. Res. Commun. 4, 272–275 (1962)
Hugo, W. B., Frier, M.: Mode of action of the antibacterial compound dequalinium acetate. Appl. Microbiol. 17, 118–127 (1970)
Perlman, P. S., Mahler, H. R.: Molecular consequences of ethidium bromide mutagenesis. Nature (Lond.) New Biol. 231, 12–16 (1971)
Pestka, S.: Inhibitors of ribosome functions. Ann. Rev. Microbiol. 25, 487–592 (1971)
Rank, G. H.: A pleiotropic nuclear gene effecting functions of the mitochondrial inner membrane of S. cerevisiae: Tetrad analyses of chloramphenicol and oligomycin resistance (submitted)
Rank, G. H., Bech-Hansen, N. T.: Somatic segregation, recombination, asymmetrical distribution and complementation tests of cytoplasmically-inherited antibiotic-resistance mitochondrial markers in S. cerevisiae. Genetics 72, 1–15 (1972)
Till, J. E., Baker, R. M., Brunette, D. M., Ling, V., Thompson, L. H., Wright, J. A.: Genetic regulation of membrane function in mammalian cells in culture. Fed. Proc. 32, 29–33 (1973)
Tzagoloff, A., Maegher, P.: Assembly of the mitochondrial membrane system. VI. Mitochondrial synthesis of subunit proteins of the rutamycin-sensitive adenosine triphosphatase. J. biol. Chem. 247, 594–603 (1972)
Walter, P., Lardy, A., Johnson, D.: Antibiotics as a tool for metabolic studies. X. Inhibition of phosphoryl transfer reactions in mitochondria by peliomycin, ossamycin, and venturicidin. J. biol. Chem. 242, 5014–5018 (1967)
Wiseman, D.: The effect of cetyltrimethylammonium bromide on the cytochrome system of Escherichia coli. J. Pharm. Pharmacol. 23, 257S (1971)
Author information
Authors and Affiliations
Additional information
Communicated by G. Magni
Rights and permissions
About this article
Cite this article
Rank, G.H., Bech-Hansen, N.T. Single nuclear gene inherited cross resistance and collateral sensitivity to 17 inhibitors of mitochondrial function in S. cerevisiae . Molec. gen. Genet. 126, 93–102 (1973). https://doi.org/10.1007/BF00330986
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00330986