Summary
In Escherichia coli the six known genes specifying the utilization of l-fucose as carbon and energy source cluster at 60.2 min and constitute a regulon. These genes include fucP (encoding l-fucose permease), fucI (encoding l-fucose isomerase), fucK (encoding l-fuculose kinase), fucA (encoding l-fuculose 1-phosphate aldolase), fucO (encoding l-1,2-propanediol oxidoreductase), and fucR (encoding the regulatory protein). In this study the fuc genes were cloned and their positions on the chromosome were established by restriction endonuclease and complementation analyses. Clockwise, the gene order is: fucO-fucA-fucP-fucI-fucK-fucR. The operons comprising the structural genes and the direction of transcription were determined by complementation analysis and Southern blot hybridization. The fuc-PIK and fucA operons are transcribed clockwise. The fucO operon is transcribed counterclockwise. The fucR gene product activates the three structural operons in trans.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Bachmann BJ (1983) Linkage map of Escherichia coli (Edition 7). Microbiol Rev 47:180–230
Backman K, Chen Y-M, Magasanik B (1981) Physical and genetic characterization of the glnA-glnG region of the Escherichia coli chromosome. Proc Natl Acad Sci USA 78:3743–3747
Bartkus JM, Mortlock RP (1986) Isolation of a mutation resulting in constitutive synthesis of l-fucose catabolic enzymes. J Bacteriol 165:710–714
Boronat A, Aguilar J (1979) Rhamnose-induced propanediol oxidoreductase in Escherichia coli: purification, properties, and comparison with the fucose-induced enzyme. J Bacteriol 140:320–326
Boronat A, Aguilar J (1981a) Experimental evolution of propanediol oxidoreductase in Escherichia coli. Biochim Biophys Acta 672:98–107
Boronat A, Aguilar J (1981b) Metabolism of l-fucose and l-rhamnose in Escherichia coli: differences in induction of propanediol oxidoreductase. J Bacteriol 147:557–566
Chakrabarti T, Chen Y-M, Lin ECC (1984) Clustering of genes for l-fucose dissimilation by Escherichia coli. J Bacteriol 157:984–986
Chen Y-M, Lin ECC (1984a) Post-transcriptional control of l-1,2-propanediol oxidoreductase in the l-fucose pathway of Escherichia coli K-12. J Bacteriol 157:341–344
Chen Y-M, Lin ECC (1984b) Dual control of a common l-1,2-propanediol oxidoreductase by l-fucose an l-rhamnose in Escherichia coli. Bacteriol 157:828–832
Chen Y-M, Lin ECC, Ros J, Aguilar J (1983) Use of operon fusions to examine the regulation of the l-1,2-propanediol oxidoreductase gene of the fucose system in Escherichia coli K12. J Gen Microbiol 129:3355–3362
Chen Y-M, Chakrabarti T, Lin ECC (1984) Constitutive activation of l-fucose genes by an unlinked mutation in Escherichia coli. J Bacteriol 159:725–729
Chen Y-M, Zhu Y, Lin ECC (1987a) NAD-linked aldehyde dehydrogenase for aerobic utilization of l-fucose and l-rhamnose by Escherichia coli. J Bacteriol 169:3289–3294
Chen Y-M, Tobin JF, Zhu Y, Schleif RF, Lin ECC (1987b) Crossinduction of the l-fucose system by l-rhamnose in Escherichia coli. J Bacteriol 169:3712–3719
Cocks T, Aguilar J, Lin ECC (1974) Evolution of l-1,2-propanediol catabolism in Escherichia coli by recruitment of enzymes for l-fucose and l-lactate metabolism. J Bacteriol 118:83–88
Hacking AJ, Lin ECC (1976) Disruption of the fucose pathway as a consequence of genetic adaptation to propanediol as a carbon source in Escherichia coli. J Bacteriol 126:1166–1172
Hacking AJ, Lin ECC (1977) Regulatory changes in the fucose system associated with the evolution of a catabolic pathway for propanediol in Escherichia coli. J Bacteriol 130:832–838
Lin ECC (1976) Glycerol dissimilation and its regulation in bacteria. Annu Rev Microbiol 30:535–578
Lin ECC (1987) Dissimilatory pathways for sugars, polyols and carboxylates. In: Neidhardt FC, Ingraham JL, Low KB, Magasanik B, Schaechter M, Umbarger HE (eds) Escherichia coli and Salmonella typhimurium: cellular and molecular biology. Am Soc Microbiol, Washington, pp 244–284
Lin ECC, Wu TT (1984) Functional divergence of the l-fucose system in mutants of Escherichia coli. In: Mortlock RP (ed) Microorganisms as model systems for studying evolution. Plenum, New York, pp 134–165
Lopilato J, Garwin JL, Emr SD, Silhavy TJ, Beckwith JR (1984) d-ribose metabolism in Escherichia coli K-12: genetics, regulation and transport. J Bacteriol 158:665–673
Lopilato J, Bortner S, Beckwith J (1986) Mutations in a new chromosomal gene of E. coli, pcnB, reduce plasmid copy number of pBR322 and its derivatives. Mol Gen Genet 205:285–290
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning, a laboratory manual. Cold Spring Harbor Laboratory, New York
Mekalanos JJ (1983) Duplication and amplification of toxin genes in Vibrio cholerae. Cell 35:253–263
Power J (1967) The l-rhamnose genetic system in Escherichia coli K-12. Genetics 55:557–568
Silhavy TJ, Berman ML, Enquist LW (1984) Experiments with gene fusions. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
Skjold AC, Ezekiel DH (1982a) Analysis of lambda insertions in the fucose utilization region of Escherichia coli K-12: use of fuc and argA transducing bacteriophages to partially order the fucose utilization genes. J Bacteriol 152:120–125
Skjold AC, Ezekiel DH (1982b) Regulation of d-arabinose utilization in Escherichia coli K-12. J Bacteriol 152:521–523
Sridhara S, Wu TT (1969) Purification and properties of lactaldehyde dehydrogenase in Escherichia coli. J Biol Chem 244:5233–5238
Sridhara S, Wu TT, Chused TM, Lin ECC (1969) Ferrous-activated nicotinamide adenine dinucleotide-linked dehydrogenase from a mutant of Escherichia coli capable of growth on 1,2-propanediol. J Bacteriol 98:87–95
Sutcliffe JG (1979) Complete nucleotide sequence of the Escherichia coli plasmid pBR322. Cold Spring Harbor Symp Quant Biol 43:77–90
Tanaka S, Lerner SA, Lin ECC (1967) Replacement of a phosphoenolpyruvate-dependent phosphotransferase by nicotinamide adenine dinucleotide-linked dehydrogenase for the utilization of mannitol. J Bacteriol 93:642–648
Yanisch-Perron C, Vieira J, Messing J (1985) Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33:103–109
Zagalak B, Frey PA, Karabatsos GL, Abeles RH (1966) The stereochemistry of the conversion of d and l 1,2-propanediols to propionaldehyde. J Biol Chem 241:3028–3035
Zhu Y, Lin ECC (1986) An evolvant of Escherichia coli that employs the l-fucose pathway also for growth on l-galactose and d-arabinose. J Mol Evol 23:259–266
Zhu Y, Lin ECC (1987) Loss of aldehyde dehydrogenase in an Escherichia coli mutant selected for growth onthe rare sugar l-galactose. J Bacteriol 169:785–789
Author information
Authors and Affiliations
Additional information
Communicated by J.W. Lengeler
Rights and permissions
About this article
Cite this article
Chen, Y.M., Zhu, Y. & Lin, E.C.C. The organization of the fuc regulon specifying l-fucose dissimilation in Escherichia coli K12 as determined by gene cloning. Molec Gen Genet 210, 331–337 (1987). https://doi.org/10.1007/BF00325702
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00325702