Summary
The development of bacterial infections is a common complication during treatment with high concentrations of oxygen. To study the effect of hyperoxia on phagocytes, the adherence, chemotaxis, ingestion rates, degranulation as well as the bactericidal activity were measured in alveolar macrophages (AMs) and polymorphonuclear leukocytes (PMNs) obtained from guinea pigs exposed to 85% oxygen. The animal exposure to a Fi O2 of 85% impaired the adherence to nylon-wool, the chemotactic activity and the phagocytic rate of paraffinoil-droplets of AMs and PMNs. In AMs the secretion of β-glucuronidase upon stimulation with opsonized zymosan was also diminished. In addition, the bactericidal activity of AMs and PMNs demonstrated a reduction of 50%. These phagocytic defects may be caused by a cytoskeleton alteration, induced by the increase of oxygen derived metabolites, representing an additional sepsis promoting factor during hyperoxia.
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Autor AP, Frank L, Roberts RJ (1976) Developmental characteristics of pulmonary superoxide dismutase: Relationship to idiopathic respiratory distress syndrome. Pediatr Res: 10 154
Baehner RL, Boxer LA, Allen JM, Davis J (1977) Autooxidation as a basis for altered function by PMN. Blood 50: 327
Biggar WD, Buran S, Holmes B (1976) Bacterial mechanisms in rabbit alveolar macrophages: evidence against peroxidase and hydrogen peroxide bacterial mechanisms. Infect Immun 14: 6
Bontan BW, Gowron R, Warshaw JB (1977) Neonatal redcell superoxide dismutase enzyme levels: Possible role as a cellular defense mechanism against pulmonary oxygen toxicity. Pediatr Res 11: 754
Boxer LA, Rister M, Allen JA, Baehner RL (1977) Improvement of Chediak-Higashi-leukocyte function by cyclic guanosine monophosphate. Blood 49: 9
Clark JM (1974) The toxicity of oxygen. Am Rev Respir Dis 110: 40
Crapo JD, Tierney DF (1974) Superoxide dismutase and pulmonary oxygen toxicity. Am J Physiol 226: 1401
Duc G (1975) Behandlung der neonatalen Hypoxie. Pädiatr Praxis 15 107
Feeney WH, Bermann ER (1978) Oxygen toxicity: Membrane damage by free radicals. Invest Ophthalmol 15: 789
Fisher AB, Diamond S, Mellen S (1974) Effect of O2 exposure on metabolism of the rabbit alveolar macrophage. J Appl Physiol 37: 341
Flohé L (1971) Die Glutathionperoxidase. Enzymologie und biologische Aspekte. Klin Wochenschr 12: 669
Fridovich L (1970) Quantitative aspects of the production of superoxide anion radical by mild xanthine oxidase. J Biol Chem 245: 4053
Fridovich I (1972) Superoxide radical and superoxide dismutase. Acc Chem Res 5: 321
Fridovich I (1975) Oxygen: Boon and bane. Am Sci 63: 54
Hill HR, Hogan NA, Thomas GM (1975) Evaluation of a cytocentrifuge method for measuring neutrophil granulocyte chemotaxis. J Lab Clin Med 86: 703
Huber T, La Force GLF (1970) Comparative effects of ozone and oxygen on pulmonary antibacterial defense mechanisms. Antimicrob Agents Chemother 10: 129
Keuth U, Feldmann EM, Althaus W, Fries N, Zemke M (1975) Erfolgreiche Behandlung neonataler Ateminsuffizienz mit kontinuierlich erhöhter transalveolärer Druckdifferenz. Pädiatr Praxis 16: 445
Ludwin SU, Northway WH, Bonson KG (1975) Oxygen toxicity in the newborn, necrotzing bronchiolitis in mice exposed to 100% oxygen. Lab Invest 31: 425
Malawista SE (1975) Microtubules and the mobilization of Lysosomes in phagocytizing human leukocytes. Ann NY Acad Sci 253: 738
Maxwell US (1964) In situ method for harvesting guinea pig alveolar macrophages. Am Rev Respir Dis 89: 579
McCord JM, Fridovich I (1969) Superoxide dismutase. An enzyme function for erythrocuprein (hemocuprein). J Biol Chem 244: 6049
McCord JM, Keele Jr BB, Fridovich I (1971) An enzyme-based theory of obligate anaerobiasis: The physiological function of superoxide dismutase. Proc Natl Acad Sci USA 68: 1024
McGregor RR, Spagnnolo PJ, Lentek AA (1974) Inhibition of granulocyte adherence by ethanol, prednisone, and aspirin measured with an assay system. N Engl J Med 291: 642
McGregor RR (1977) Granulocyte adherence changes induced by hemodialysis, endotoxin, epinephrine and glucocorticoids. Ann Intern Med 86: 35
McGregor RR, Macarak EJ, Kefalides NA (1978) Comparative adherence of granulocytes to endothelial monolayers and nylon fiber. J Clin Invest 61: 697
Misra HP, Fridovich I (1971) The generation of superoxide radical during the autooxidation of ferridoxins. J Biol Chem 246: 6886
Northway WH, Rosan RC, Porter DY (1967) Pulmonary disease following respirator therapy of hyaline-membrane disease. N Engl J Med 276: 357
Northway WH, Rezau L, Petriceks R, Bensch K (1976) Oxygen toxicity in the newborn lung: Reversal of inhibition of DNA synthesis in the mouse. Pediatrics 57: 41
Oliver JM, Albertini DF, Berlin RD (1976) Effects of Glutathione-Oxidizing agents on microtubule assembly and microtubule-dependent surface properties of human neutrophils. J Cell Biol 71: 921
Oliver JM, Zurier RB (1976) Correction of characteristic abnormalities of microtubule function and granule morphology in Chediak-Higashi-syndrome with cholinergic agonists. J Clin Invest 57: 1239
Pratt PC (1974) Pathology of pulmonary oxygen toxicity. Am Rev Respir Dis 110: 51
Quie PG, White JG, Holmes B, Good RA (1967) In vitro bactericidal capacity of human polymorphonuclear leukocytes: Diminished activity in chronic granulomatous disease of childhood. J Clin Invest 46: 688
Rister M, Baehner RL (1976) A comparative study of superoxide dismutase activity in polymorphonuclear leukocytes, monocytes, and alveolar macrophages of the guinea pig. J Cell Physiol 87: 345
Rister M, Baehner RL (1976) The alteration of superoxide dismutase, catalase, glutathione peroxidase, and NAD (P) H cytochrome C reductase in guinea pig polymorphonuclear leukocytes and alveolar macrophages during hyperoxia. J Clin Invest 58: 117
Rister M, Baehner RL (1977) Effects of hyperoxia on superoxide anion and hydrogen peroxide production of polymorphonuclear leukocytes and alveolar macrophages. Br J Haematol 36: 241
Rister M, Baehner RL (1978) Neue Aspekte zur Toxizität des Sauerstoffs. Dtsch Med Wochenschr 23: 977
Rister M (1980) Phagozytosemechanismen alveolärer Makrophagen und Granulozyten. Blut 41: 257
Rister M (1980) The cellular basis of oxygen toxicity (Abstract). Pediatr Res 14: 167A
Rosenbaum RM, Wittner M, Lenger M (1969) Mitochondrial and other ultrastructural changes in great alveolar cells of oxygen-adapted and poisoned rats. Lab Invest 20: 516
Stopfkuchen H, Eckert H-G, Emmrich P, Tröger J (1979) Komplikationen und Überlebensraten bei mechanisch beatmeten Früh-und Neugeborenen. Monatschr Kinderheilkd 127: 454
Stossel TP (1973) Evaluation of opsonic and leukocyte function with a spectrophotometric test in patients with infection and phagocytic disorders. Blood 42: 121
Wolff LJ, Boxer LA, Allen JM, Baehner RL (1978) The selective effect of hyperoxia on the guinea pig. J Reticuloendothel Soc 24: 381
Yamamoto E, Wittner M, Rosenbaum RM (1970) Resistance and susceptibility to oxygen toxicity by cell types of the gas-blood barriw of the rat lung. Am J Pathol 59: 409
Zurier RB, Weismann G, Hoffstein S, Kammermann S, Tai HH (1974) Mechanismus of lysosomal enzyme release from human leukocytes. II. Effects of cAMP and cGMP, autonomic agonists, and agents which affect microtubule function. J Clin Invest 53: 297
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Rister, M. Effects of hyperoxia on phagocytosis. Blut 45, 157–166 (1982). https://doi.org/10.1007/BF00320800
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DOI: https://doi.org/10.1007/BF00320800