Summary
By a brief exposure of small intestine (mouse, rat, guinea pig) to lysolecithin prior to vital methylene blue staining we were able to demonstrate in a selective way the complete network of interstitial cells of Cajal, located in the space between the longitudinal and circular muscle layers. A combination with fluorescence labeling (FITC-dextran uptake) of macrophage-like cells, allowed us to demonstrate 1) the complete, regular distribution of both cell populations along the entire small intestine, and 2) the constant, intimate associations between interstitial cells of Cajal and macrophage-like cells.
In relation to current hypotheses concerning a role of interstitial cells of Cajal in motility regulation, our results call attention to the possible involvement of another cell type, the macrophage-like cell.
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Arthur RP, Shelley WB (1959) The technology of in vitro staining of nerves in human skin with thiazin dyes. J Invest Dermatol 33:121–144
Cajal SR (1893) Sur les ganglions et plexus nerveux de l'intestin. Compt Rend Soc Biol (Paris) 45:217–223
Daniel EE (1977) Nerves and motor activity of the gut. In: Brooks FB, Evers PW (eds) Nerves and the gut. CB Slack New York, pp 154–196
Daniel EE, Posey-Daniel V (1984) Neuromuscular structures in opossum esophagus: role of interstitial cells of Cajal. Am J Physiol 246 (Gastrointest Liver Physiol 9): G305–315
Durdle NG, Kingma YJ, Bowes KL, Chambers MM (1983) Origin of slow waves in the canine colon. Gastroenterology 84:375–382
Faussone-Pellegrini MS, Cortesini C (1983) Some ultrastructural features of the muscular coat of human small intestine. Acta Anat 115:47–68
Gabella G (1987) The number of neurons in the small intestine of mice, guinea pigs and sheep. Neuroscience 22:737–752
Hara Y, Kubota M, Szurszewski JH (1986) Electrophysiology of smooth muscle of the small intestine of some mammals. J Physiol 372:501–520
Hurley CK, Giles RC, Capra JD (1983) The human MHC: evidence for multiple HLA-D-region genes. Immunology Today 4:219–226
Mikkelsen HB, Thuneberg L, Rumessen JJ, Thorball N (1985) Macrophage-like cells in muscularis externa of mouse small intestine. Anat Rec 213:77–86
Mikkelsen HB, Mirsky R, Jessen KR, Thuneberg L (1988) Macrophage-like cells in muscularis externa of mouse small intestine: immunohistochemical localization of F4/80, M1/70 and Ia-antigen. Cell Tissue Res (in press)
Richardson KC (1969) The fine structure of autonomic nerves after vital staining with methylene blue. Anat Rec 164:359–378
Rumessen JJ, Thuneberg L (1982) Plexus muscularis profundus and associated interstitial cells. I. Light microscopical studies of mouse small intestine. Anat Rec 203:115–127
Rumessen JJ, Thuneberg L, Mikkelsen HB (1982) Plexus muscularis profundus and associated interstitial cells. II. Ultrastructural studies of mouse small intestine. Anat Rec 203:129–146
Smith TK, Reed JB, Sanders KM (1987) Origin and propagation of electrical slow waves in circular muscle of canine proximal colon. Am J Physiol 252 (Cell Physiol 21):C215-C224
Stefanini M, De Martino C, Zamboni L (1967) Fixation of ejaculated spermatozoa for electron microscopy. Nature 216:173–174
Suzuki N, Prosser CL, Dahms V (1986) Boundary cells between longitudinal and circular layers: essential for electrical slow waves in cat intestine. Am J Physiol 250 (Gastrointest Liver Physiol 13):G287-G294
Taxi J (1965) Contribution à l'étude des connexions des neurones moteurs du systeme nerveux autonome. Ann Sci Nat Zool 7:413–674
Thuneberg L (1982) Interstitial cells of Cajal: intestinal pacemaker cells? Adv Anat Embryol Cell Biol 71:1–130
Thuneberg L (1988) Interstitial cells of Cajal. In: Wood JD (ed) Handbook of Physiology (Gastrointestinal Physiology) (in press)
Unanue ER (1984) Antigen-presenting function of the macrophage. Ann Rev Immunol 2:395–428
Weltzier HV (1979) Cytolytic and membrane perturbing properties of lysophosphatidylcholine. Biochim Biophys Acta 559:259–287
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A preliminary account of this study was published in the proc. of The XIth Symp. Gastrointest. Motil., Oxford, 1987, in: Digest. Dis. Sci. 32:922
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Mikkelsen, H.B., Thuneberg, L. & Wittrup, I.H. Selective double staining of interstitial cells of Cajal and macrophage-like cells in small intestine by an improved supravital methylene blue technique combined with FITC-dextran uptake. Anat Embryol 178, 191–195 (1988). https://doi.org/10.1007/BF00318222
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DOI: https://doi.org/10.1007/BF00318222