Summary
Restriction fragment length polymorphisms have been used to determine the chromosomal location of the genes encoding the glycine decarboxylase complex (GDC) and serine hydroxymethyltransferase (SHMT) of pea leaf mitochondria. The genes encoding the H subunit of GDC and the genes encoding SHMT both show linkage to the classical group I marker i. In addition, the genes for the P protein of GDC show linkage to the classic group I marker a. The genes for the L and T proteins of GDC are linked to one another and are probably situated on the satellite of chromosome 7. The mRNAs encoding the five polypeptides that make up GDC and SHMT are strongly induced when dark-grown etiolated pea seedlings are placed in the light. Similarly, when mature plants are placed in the dark for 48 h, the levels of both GDC protein and SHMT mRNAs decline dramatically and then are induced strongly when these plants are returned to the light. During both treatments a similar pattern of mRNA induction is observed, with the mRNA encoding the P protein of GDC being the most rapidly induced and the mRNA for the H protein the slowest. Whereas during the greening of etiolated seedlings the polypeptides of GDC and SHMT show patterns of accumulation similar to those of the corresponding mRNAs, very little change in the level of the polypeptides is seen when mature plants are placed in the dark and then re-exposed to the light.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Blackwell D, Murray AJS, Lea PJ (1990) Photo respiratory mutants of the mitochondrial conversion of glycine to serine. Plant Physiol 94:1316–1322
Blake (1984) A rapid and sensitive method for detection of alkaline phosphatase-conjugated anti-antibody on Western blots. Anal Biochem 136:175–179
Blixt S (1974) The pea. In: King RC (ed) Handbook of genetics. vol 2. Plenum, New York, p 181
Bourguignon J, Neuburger M, Douce R (1988) Resolution and characterisation of the glycine-cleavage reaction in pea leaf mitochondria. Biochem J 255:169–178
Combette B, Bonnard G, Neuburger M, Douce R, Grienenberger JM (1991) Serine hydroxymethyltransferase is encoded in the mitochondrial genome of pea. Abstract 3-4 from the Third Plant Respiration Meeting, Utrecht, 1991
Day DA, Neuburger M, Douce R (1985) Biochemical characterisation of chlorophyll-free mitochondria from pea leaves. Aust J Plant Physiol 12:219–228
Ellis THN, Turners L, Hellens RP, Lee D, Harker CL, Enard C, Domoney C, Davies DR (1992) Linkage maps in pea. Genetics 130:649–663
Gilmartin PM, Sarokin L, Memelink J, Chua N (1990) Molecular light switches for plant genes. Plant Cell 2:369–378
Haldane JBS, Waddington CH (1931) Inbreeding and linkage. Genetics 16:357–374
Hiraga K, Kikuchi G (1980) The mitochondrial glycine cleavage system. J Biol Chem 255:11664–11670
Laemmli UK (1970) Cleavage of structural proteins during the the assembly of the head of bacteriophage T4. Nature 227:680–685
Kikuchi G (1973) The glycine cleavage system: composition, reaction mechanism, and physiological significance. Mol Cell Biochem 1:169–187
Kim Y, Oliver DJ (1990) Molecular cloning, transcriptional characterisation, and sequencing of cDNA encoding the H-protein of the mitochondrial glycine decarboxylase complex in peas. J Biol Chem 265:848–853
Kure S, Koyata H, Kume A, Ishiguro Y, Hiraga K (1991) The glycine cleavage system. The coupled expression of the glycine decarboxylase gene and the H-protein gene in the chicken. J Biol Chem 266:3330–3334
Macherel D, Lebrun M, Gagnon J, Neuburger M, Douce R (1990) cDNA cloning, primary structure and gene expression for Hprotein, a component of the glycine-cleavage system (glycine decarboxylase) of pea (Pisum sativum) leaf mitochondria. Biochem J 268:783–789
Morgan C, Turner SR, Rawsthorne S (1992) The cell-specific distribution of glycine decarboxylase in the leaves of C3, C4 and C3–C4 intermediate species. In: Lambers JT, van der Plaas LHW (eds) Molecular, biochemical and physiological aspects of plant respiration. SPB Academic Publishing, The Hague, in press
Neuburger M, Bourguignon J, Douce R (1986) Isolation of a large complex from the matrix of pea leaf mitochondria involved in the rapid transformation of glycine to serine. FEBS Lett 207:18–22
Okamura-Ikeda K, Fujiwara K, Motokawa Y (1982) Purification and characterisation of chicken liver T-protein, a component of the glycine cleavage system. J Biol Chem 257:135–139
Okamura-Ikeda K, Fujiwara K, Yamamoto K, Hiraga K, Motokawa Y (1991) Purification and characterisation of chicken liver T-protein, a component of the glycine cleavage system. J Biol Chem 266:4917–4921
Oliver DJ, Neuburger M, Bourguignon J, Douce R (1990) Interaction between the components of the glycine decarboxylase multisubunit complex. Plant Physiol 94:833–839
Shirch V, Peterson D (1980) Purification and properties of mitochondrial serine hydroxymethyltransferase. J Biol Chem 255:7801–7806
Somerville CR, Ogren WL (1982a) Mutants of Arabidopsis thaliana lacking glycine decarboxylase activity. Biochem J 202:373–380
Somerville CR, Ogren WL (1982b) Photorespiration-deficient mutants of Arabidopsis thaliana lacking mitochondria) serine transhydroxymethylase activity. Plant Physiol 67:666–671
Turner SR, Ireland R, Rawsthorne S (1992a) The cloning and characterisation of the P subunit of glycine decarboxylase from pea (Pisum sativum). J Biol Chem 267:5355–5360
Turner SR, Ireland R, Rawsthorne S (1992b) The cloning and characterisation of a cDNA encoding the L subunit of glycine decarboxylase from pea (Pisum satium). J Biol Chem 267:7745–7750
Turner SR, Ireland R, Rawsthorne S (1992c) Identification and localisation of multiple forms of serine hydroxymethyltransferase in pea (Pisum sativum) and characterisation of a cDNA encoding a mitochondrial isoform. J Biol Chem 267:13528–13534
Walker JL, Oliver DJ (1986a) Glycine decarboxylase multienzyme complex: purification and partial characterisation from pea leaf mitochondria. J Biol Chem 5:2214–2221
Walker JL, Oliver DJ (1986b) Light-induced increases in the glycine decarboxylase multienzyme complex from pea leaf mitochondria. Arc Biochem Biophys 248:626–638
Weeden NF, Wolko B (1990) Linkage map for the garden pea (Pisum sativum). In O'Brien SJ (ed) Genetic maps. Locus maps of complex genomes, 5th edn. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, pp 106–112
Author information
Authors and Affiliations
Additional information
Communicated by D.M. Lonsdale
Rights and permissions
About this article
Cite this article
Turner, S.R., Hellens, R., Ireland, R. et al. The organisation and expression of the genes encoding the mitochondrial glycine decarboxylase complex and serine hydroxymethyltransferase in pea (Pisum sativum). Molec. Gen. Genet. 236, 402–408 (1993). https://doi.org/10.1007/BF00277140
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00277140