Summary
The entire set of transferred chloroplast DNA sequences in the mitochondrial genome of rice (Oryza sativa cv. Nipponbare) was identified using clone banks that cover the chloroplast and mitochondrial genomes. The mitochondrial fragments that were homologous to chloroplast DNA were mapped and sequenced. The nucleotide sequences around the termini of integrated chloroplast sequences in the rice mtDNA revealed no common sequences or structures that might enhance the transfer of DNA. Sixteen chloroplast sequences, ranging from 32 bases to 6.8 kb in length, were found to be dispersed throughout the rice mitochondrial genome. The total length of these sequences is equal to approximately 6% (22 kb) of the rice mitochondrial genome and to 19% of the chloroplast genome. The transfer of segments of chloroplast DNA seems to have occurred at different times, both before and after the divergence of rice and maize. The mitochondrial genome appears to have been rearranged after the transfer of chloroplast sequences as a result of recombination at these sequences. The rice mitochondrial DNA contains nine intact tRNA genes and three tRNA pseudogenes derived from the chloroplast genome.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Bowman CM, Barker RF, Dyer TA (1988) In wheat ctDNA, segments of ribosomal protein genes are dispersed repeats, probably conserved by nonreciprocal recombination. Curr Genet 14:127–136
Ellis J (1982) Promiscuous DNA — chloroplast genes inside plant mitochondria. Nature 299:678–679
Gantt JS, Baldauf SL, Calie PJ, Weeden NF, Palmer JD (1991) Transfer of rpl22 to the nucleus greatly preceded its loss from the chloroplast and involved the gain of an intron. EMBO J 10:3073–3078
Hirai A, Ishibashi T, Morikami A, Iwatsuki N, Shinozaki K, Sugiura M (1985) Rice chloroplast DNA: a physical map and the location of the genes for the large subunit of ribulose-1,5-bisphosphate carboxylase and the 32KD photosystem II reaction center protein. Theor Appl Genet 70:117–122
Hiratsuka J, Shimada H, Whittier R, Ishibashi T, Sakamoto M, Mori M, Kondo C, Honji Y, Sun C, Meng B, Li Y, Kanno A, Nishizawa Y, Hirai A, Shinozaki K, Sugiura M (1989) The complete sequence of the rice (Oryza sativa) chloroplast genome: Intermolecular recombination between distinct tRNA genes accounts for a major plastid DNA inversion during the evolution of the cereals. Mol Gen Genet 217:185–194
lams KP, Heckman JE, Sinclair JH (1985) Sequence of histidyl tRNA, present as a chloroplast insert in mtDNA of Zea mays. Plant Mol Biol 4:225–232
Iwahashi M, Nakazono M, Kanno A, Sugino K, Ishibashi T, Hirai A (1992) Genetic and physical maps and a clone bank of mitochondrial DNA from rice. Theor Appl Genet 84:275–279
Joyce PBM, Gray MW (1989) Chloroplast-like transfer RNA genes expressed in wheat mitochondria. Nucleic Acids Res 17:5461–5476
Kemble RJ, Mans RJ, Gabay-Laughnan S, Laughnan JR (1983) Sequence homologous to episomal mitochondrial DNAs in the maize nuclear genome. Nature 304:744–747
Lonsdale DM, Hodge TP, Howe CJ, Stern DB (1983) Maize mitochondrial DNA contains a sequence homologous to the ribulose-1,5-bisphosphate carboxylase large subunit gene of chloroplast DNA. Cell 34:1007–1014
Manna E, Brennicke A (1986) Site-specific circularisation at an intragenic sequence in Oenothera mitochondria. Mol Gen Genet 203:377–381
Maréchal-Drouard L, Guillemaut P, Cosset A, Arbogast M, Weber F, Weil J-H, Dietrich A (1990) Transfer RNAs of potato (Solanum tuberosum) mitochondria have different genetic origins. Nucleic Acids Res 18:3689–3696
Moon E, Kao T-H, Wu R (1988) Rice mitochondrial genome contains a rearranged chloroplast gene cluster. Mol Gen Genet 213:247–253
Newton KJ (1988) Plant mitochondrial genomes: organization, expression and variation. Annu Rev Plant Physiol Plant Mot Biol 39:503–532
Nugent JM, Palmer JD (1988) Location, identity, amount and serial entry of chloroplast DNA sequences in crucifer mitochondrial DNAs. Curr Genet 14:501–509
Nugent JM, Palmer JD (1991) RNA-mediated transfer of the gene coxII from the mitochondrion to the nucleus during flowering plant evolution. Cell 66:473–481
Palmer JD, Herbon LA (1988) Plant mitochondrial DNA evolves rapidly in structure, but slowly in sequence. J Mot Evol 28:87–97
Schuster W, Brennicke A (1987a) Plastid, nuclear and reverse transcriptase sequences in the mitochondrial genome of Oenothera: is genetic information transferred between organelles via RNA? EMBO J 6:2857–2863
Schuster W, Brennicke A (1987b) Plastid DNA in the mitochondriat genome of Oenothera: Intra- and interorganellar rearrangements involving part of the plastid ribosomal cistron. Mol Gen Genet 210:44–51
Schuster W, Brennicke A (1988) Interorganellar sequence transfer: Plant mitochondrial DNA is nuclear, is plastid, is mitochondrial. Plant Science 54:1f10
Stern DB, Lonsdale DM (1982) Mitochondrial and chloroplast genomes of maize have a 12-kilobase DNA sequence in common. Nature 299:698–702
Stern DB, Palmer JD (1984) Extensive and widespread homologies between mitochondrial DNA and chloroplast DNA in plant. Proc Natl Acad Sci USA 81:1946–1950
Stern DB, Palmer JD (1986) Tripartite mitochondrial genome of spinach: physical structure, mitochondrial gene mapping, and locations of transposed chloroplast DNA sequences. Nucleic Acids Res 14:5651–5666
Timmis JN, Scott NS (1983) Spinach nuclear and chloroplast DNAs have homologous sequences. Nature 305:65–67
Author information
Authors and Affiliations
Additional information
Communicated by R.G. Herrmann
Rights and permissions
About this article
Cite this article
Nakazono, M., Hira, A. Identification of the entire set of transferred chloroplast DNA sequences in the mitochondrial genome of rice. Molec. Gen. Genet. 236, 341–346 (1993). https://doi.org/10.1007/BF00277131
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00277131