Abstract
Compact, friable and embryogenic calli were initiated from immature inflorescences and young leaf bases of one week old seedlings of Paspalum scrobiculatum cultured on MS medium supplemented with 2,4-D. A stable, embryogenic suspension culture was initiated from these calli and maintained in a liquid version of the same MS medium. Embryogenic calli and somatic embryos were obtained by plating suspension culture cells onto semi-solid medium containing 2,4-D. Complete, normal plantlets developed on 2,4-D free medium at a high frequency from somatic embryos. NAA and BAP in the medium promoted plant development.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
Abbreviations
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
- NAA:
-
1-naphthaleneacetic acid
- BAP:
-
6-benzylaminopurine
- ABA:
-
Abscisic acid
- MS:
-
Murashige and Skoog (1962)
- CM:
-
Coconut milk
Reference
Abdullah R, Cocking EC, Thompson JA (1986) Bio/Technology 4: 1087–1090
Bhattacharya P, Sen SK (1980) Theor Appl Genet 56: 87–90
Coulibaly MY, Demarly Y (1986) Z Pflanzenzuchtg 96: 79–81
Deka PC, Sen SK (1976) Mol Gen Genet 145: 239–244
Fujimura T, Sakurai M, Agaki H, Negishi T, Hirose A (1985) Plant Tissue Culture Lett 2: 74–75
Halperin W (1968) Amer J Bot 53: 443–453
Karlsson SB, Vasil IK (1986) Amer J Bot 73: 894–901
Kyozuka J, Hayashi Y, Shimamoto K (1987) Mol Gen Genet 206: 408–413
Kyozuka J, Otoo E, Shimamoto K (1988) Theor Appl Genet 76: 887–890
Lu C, Vasil IK (1981) Ann Bot 47: 543–548
Murashige T, Skoog F (1962) Physiol Plant 15: 473–497
Nayak P, Sen SK (1987) In: Proc Symp Plant Cell & Tissue Culture of Economically Important Plants (ed. GM Reddy) Univ Osmania, Hyderabad pp 217–222
Toriyama K, Hinata K, Sasaki T (1986) Theor Appl Genet 73: 16–19
Vasil IK (1985) In: Tissue Culture in Forestry and Agriculture (ed. Randolph R Hanka, Karen W Hughes, Milton P Constantin, Alexander Hollaender) Plenum, New York pp 31–47
Vasil IK (1987) J Plant Physiol 128: 193–218
Vasil V, Vasil IK (1981) Ann Bot 47: 669–678
Vasil V, Vasil IK (1982) Amer J Bot 69: 1441–1449
Yamada M, Yang ZQ, Tang DT (1986) Plant Cell Rep 5: 85–88
Author information
Authors and Affiliations
Additional information
Communicated by G. C. Phillips
Rights and permissions
About this article
Cite this article
Nayak, P., Sen, S.K. Plant regeneration through somatic embryogenesis from suspension cultures of a minor millet, Paspalum scrobiculatum . Plant Cell Reports 8, 296–299 (1989). https://doi.org/10.1007/BF00274134
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00274134