Summary
Developmental changes in the distribution of parvalbumin-specific immunoreactivity in the brain, in particular in the cerebral cortex and hippocampus, were followed immunohistochemically in two different species, the rat and the Mongolian gerbil (Meriones unguiculatus) using an antibody raised against for rat parvalbumin. The gerbil is known to develop its auditory and visual capacity later than rat. In both the rat and gerbil, parvalbumin-specific immunoreactivity appeared after birth in both the cerebral cortex and hippocampus. The timing of the development of expression of parvalbumin varied among different parts of the cerebral cortex. The parietal cortex showed evidence of the earliest expression of parvalbumin whilst the occipital and temporal cortices expressed parvalbumin at a later stage of a development. This feature was common to both the rat and gerbil but occurred at a relatively later stage in the gerbil. The profile of the distribution of parvalbumin in the brain of the developing and adult gerbil was similar to that of the rat, but there were some differences. The frequency of bead-like structures on the dendrites of the parvalbumin-positive cells in the CA1 region of the hippocampus was markedly lower in the gerbil; instead, straight non-beaded fibers which ran vertically into the pyramidal layer were stained. Parvalbumin-positive fibers were also found in the cerebral cortex of the gerbil.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Aoki E, Semba R, Keino H, Kato-Semba R, Kashiwamata S (1985) Postnatal development of γ-aminobutyric acid (GABA) neurons in the cerebellum of jaundiced Gunn rats: a study with anti-GABA antiserum. Biomed Res 6:145–152
Berchtold MW, Celio MR, Heizmann CW (1984) Parvalbumin in non-muscle tissue of the rat. Quantitation and immunohistochemical localization. J Biol Chem 259:5189–5196
Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254
Braun K, Schachner M, Scheich H, Heizmann CW (1986) Cellular localization of the Ca2+-binding protein parvalbumin in developing avian cerebellum. Cell Tissue Res 243:69–78
Braun K, Scheich H, Schachner M, Heizmann CW (1985a) Distribution of parvalbumin, cytochrome oxidase activity and 14C-2-deoxyglucose uptake in the brain of the zebra finch. I. Auditory and vocal motor systems. Cell Tissue Res 240:101–115
Braun K, Scheich H, Zuschratter W, Heizmann CW, Matute C, Streit P (1988) Postnatal development of parvalbumin-, calbindin- and adult GABA-immunoreactivity in two visual nuclei of zebra finches. Brain Res 475:295–217
Celio MR (1990) Calbindin D-28k and parvalbumin in the rat nervous system. Neuroscience 35:375–475
Demeulemeester H, Vandesande F, Orban GA, Heizmann CW, Pochet R (1989) Calbindin D-28k and parvalbumin immunoreactivity is confined to two separate neuronal subpopulations in the cat visual cortex, whereas partial coexistence is shown in the dorsal lateral geniculate nucleus. Neurosci Lett 99:6–11
Endo T, Kobayashi S, Onaya T (1985) Parvalbumin in rat cereberum, cerebellum and retina during postnatal development. Neurosci Lett 60:279–282
Endo T, Takayama K, Kobayashi S, Onaya T (1986) Immunochemical and immunohistochemical localization of parvalbumin in rat nervous system. J Neurochem 46:892–898
Heizmann CW (1984) Parvalbumin, an intracellular calcium-binding protein; distribution, properties and possible roles in mammalian cells. Experientia 40:910–921
Heizmann CW (1988) Parvalbumin in non-muscle cells. In: Gerday Ch, Gilles R, Bolis L (eds) Proceedings in life sciences, calcium and calcium binding proteins. Springer, Berlin Heidelberg New York, pp 93–101
Heizmann CW, Braun K (1989) Calcium binding proteins. Molecular and functional aspects. In: Anghileri LJ (ed) The role of calcium in biological systems, vol. V. CRC Press, Boca Raton, Florida, pp 21–66
Heizmann CW, Celio MR (1987) Immunolocalization of parvalbumin. Methods Enzymol 139:552–570
Heizmann CW, Hunziker W (1990) Intracellular calcium-binding molecules. In: Bronner F (ed) Intracellular calcium regulation. Alan R Liss, New York, pp 211–247
Hendry SHC, Jones EG, Emson PC, Lawson DEM, Heizmann CW (1989) Two classes of cortical GABA neurons defined by differential calcium binding protein immunoreactivities. Exp Brain Res 76:467–472
Kägi U, Berchtold MW, Heizmann CW (1987) Ca2+-binding protein in rat brain. Characterization, localization and expression during development. J Biol Chem 262:7314–7320
Katsumaru H, Kosaka T, Heizmann CW, Hama K (1988) Immunocytochemical study of GABAergic neurons containing the calcium-binding protein parvalbumin in the rat hippocampus. Exp Brain Res 72:347–362
Kawaguchi Y, Katsumaru H, Kosaka T, Heizmann CW, Hama K (1987) Fast spiking cells in rat hippocampus (CA1 region) contain the calcium-binding protein parvalbumin. Brain Res 416:369–374
Kosaka T, Katsumaru H, Hama K, Wu J-Y, Heizmann CW (1987a) GABAergic neurons containing the Ca2+-binding protein parvalbumin in the rat hippocampus and dentate gyrus. Brain Res 419:119–130
Kosaka T, Heizmann CW, Tateishi K, Hamaoka Y, Hama K (1987b) An aspect of the organizational principle of the γ-aminobutyric acid-ergic system in the cerebral cortex. Brain Res 409:403–408
Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685
Loskota WJ, Lomax P (1975) The Mongolian gerbil (Meriones unguiculatus) as a model for the study of epilepsies. EEG records of seizures. Electroencephalogr Clin Neurophysiol 38:597–604
Morris RJ, Beech JN, Heizmann CW (1988) Two distinct phases and mechanisms of axonal growth shown by primary vestibular fibers in the brain. An immunohistochemical study using antibodies to parvalbumin. Neuroscience 27:597–605
Schmechel DE, Brightman MW, Marangos PJ (1980) Neurons switch from non-neuronal enolase to neuron-specific enolase during differentiation. Brain Res 190:195–214
Seto-Ohshima A, Aoki E, Semba R, Emson PC, Heizmann CW (1989) Parvalbumin immunoreactivity in the reticular thalamic nucleus of developing rats. Acta Histochem Cytochem 22:331–340
Stichel CC, Kägi U, Heizmann CW (1986) Parvalbumin in cat brain: isolation, characterization, and localization. J Neurochem 47:46–53
Straus W (1982) Imidazole increases the sensitivity of the cytochemical reaction for peroxidase with diaminobenzidine at a neutral pH. J Histochem Cytochem 30:491–493
Towbin H, Staehelin T, Gordon J (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose. Proc Natl Acad Sci USA 76:4350–4354
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Seto-Ohshima, A., Aoki, E., Semba, R. et al. Appearance of parvalbumin-specific immunoreactivity in the cerebral cortex and hippocampus of the developing rat and gerbil brain. Histochemistry 94, 579–589 (1990). https://doi.org/10.1007/BF00271984
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00271984