Abstract
Extracts of Artemisia annua cultures have been assessed for in vitro activity against the malarial parasite Plasmodium falciparum. Callus and suspension cells and medium were analysed and examined for their activity at different stages of growth and development. Time-course experiments were carried out to investigate the influence of various basal media, plant growth regulators and light on both growth and possible artemisinin production. Two active fractions were obtained but artemisinin was not detected.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Anon. (1979) Chin. Med. J. 92, 811–816
Anon. (1981) Fourth meeting of Scientific Working Group on the chemotherapy of malaria, Beijing, China, WHO Report TDR/CHEMAL-SWG (4) QHS/81:3
Desjardins RE, Canfield CJ, Haynes JD, Chulay JD (1979) Antimicrob. Ag. Chemother. 16, 710–718
Fairlamb AH, Warhurst DC, Peters W (1985) Ann. Trop. Med. Parasit. 79: 379–384
Fowler MW (1986) In Yeoman MM (ed) “Plant cell culture technology”, Botanical Monographs 23, Blackwell, Oxford, p 202
Fowler MW (1983) In Mantell SH and Smith H (eds) “Plant Biotechnology”, Cambridge University Press Cambridge, p 3
Gamborg O, Miller RA and Ojima K (1968) Exp. Cell Res. 50: 155–158
Linsmaier EM and Skoog F (1965) Physiol. Plant 18: 100–109
Mantell SH and Smith H (1983), In Mantell SH and Smith H (eds) Plant Biotechnology, Cambridge University Press, Cambridge, pp 75–108
Murashige T and Skoog F (1962) Physiol. Plant 15: 473–497
Nair M, Acton N, Klayman DL (1986) J. Nat. Prod. 49 (3), 504–507
O'Neill MJ, Bray DH, Boardman P, Phillipson JD and Warhurst DC (1985) Planta Medica 5, 394–398
Osisanya JO, Gould S, Warhurst DC (1981) Ann. Trop. Med. and Parasit. 75, 107–109
Schenk RU and Hildebrandt AC (1972) Can. J. Bot. 50: 199–204
Thaithong S, Beale GH and Chutmongkonkul M (1983) Trans. Roy. Soc. Trop. Med. Hyg. 77: 228–231
Wyler DJ (1983) New England Journal of Medicine 308, 875–878
Xu Xing-Xiang, Zhu Jie, Huang D-Zhong, Zhou Wei-Sham (1986) Tetrahedron 42 (3), 819–828
Author information
Authors and Affiliations
Additional information
Communicated by M. H. Zenk
Rights and permissions
About this article
Cite this article
Tawfiq, N.K., Anderson, L.A., Roberts, M.F. et al. Antiplasmodial activity of Artemisia annua plant cell cultures. Plant Cell Reports 8, 425–428 (1989). https://doi.org/10.1007/BF00270085
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00270085