Abstract
Leaf discs from four strains and petioles from six strains of Gossypium hirsutum were cultured on a variety of media. Callus formed from explants on all media, though embryogenesis was highly specific. Embryos formed from only three strain x media combinations. A small percentage of these embryos developed into plantlets. These results demonstrate that cotton plants can be obtained from leaf tissue explants.
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Abbreviations
- BA:
-
benzyladenine
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
- GA3 :
-
gibberellic acid
- Kn:
-
Kinetin
- NAA:
-
1-naphthalene acetic acid
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Communicated by G. C. Phillips
College of Agricultural Sciences Publication Number T-4-193; this research was partially funded by the USDA-ARS Plant Stress and Water Conservation Research Program
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Gawel, N.J., Rao, A.P. & Robacker, C.D. Somatic embryogenesis from leaf and petiole callus cultures of Gossypium hirsutum L.. Plant Cell Reports 5, 457–459 (1986). https://doi.org/10.1007/BF00269641
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DOI: https://doi.org/10.1007/BF00269641