Abstract
Maintainable, highly embryogenic suspension cultures of cotton (Gossypium hirsutum L. cv. ‘Coker 310’) have been obtained. Callus cultures were initiated from cotyledonary tissues from aseptically-germinated seedlings. To establish the suspension cultures, callus tissue was placed in a liquid medium containing either 0.5 mg/l picloram or 0.1 mg/l 2,4-dichlorophenoxyacetic acid. For proliferation of the embryogenic suspension, 5 mg/l of 2,4-dichlorophenoxyacetic acid was used. Embryo development took place when the embryogenic tissue was transferred to an auxin-free liquid medium containing 15 mM glutamine. Early embryo development was fairly synchronous and large numbers of somatic embryos were produced. Regenerated plants were fertile and smaller than seed-derived plants.
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Abbreviations
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
- NAA:
-
1-naphthaleneacetic acid
- IAA:
-
indole-3-acetic acid
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Communicated by G. C. Phillips
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Finer, J.J. Plant regeneration from somatic embryogenic suspension cultures of cotton (Gossypium hirsutum L.). Plant Cell Reports 7, 399–402 (1988). https://doi.org/10.1007/BF00269522
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DOI: https://doi.org/10.1007/BF00269522