Summary
A sugar oxidizing enzyme which produces H2O2 during glucose starvation in the white-rot fungus Phanerochaete chrysosporium has been purified from mycelial extracts and somewhat characterized. Enzyme purity was confirmed by analytical isoelectric focusing and by dodecylsulfate/polyacrylamide gel electrophoresis, both techniques revealing a homogeneous protein. The enzyme is active over a broad pH range with maximum activity at pH 7.5. Of several sugars tested, glucose was the preferred substrate although δ-d-gluconolactone and d-xylose were also oxidized at significant rates (at 60% and 37%, respectively, of the rate observed with glucose). K m-values for glucose and xylose are 1.03 and 20 mM respectively and the glucose oxidation product was idenitified as d-arabino-2-hexosulose. The possible importance of glucose-2-oxidase in lignin degradation is discussed.
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Ander P, Eriksson K-E (1985) Methanol formation during lignin degradation by Phanerochaete chrysosporium. Appl Microbiol Biotechnol 21:96–102
Ander P, Eriksson MER, Eriksson K-E (1985) Methanol production from lignin-related substances by Phanerochaete chrysosporium. Physiol Plant, in press
Baute MA, Baute R (1984) Occurrence among macrofungi of the bioconversion of glucozone to cortalcerone. Phytochemistry 23:271–274
Baute M-A, Baute R, Deffieux G, Filleau M-J (1977) Conversion of glucose to cortalcerone via glucosone by Corticium caeruleum. Phytochemistry 16:1895–1897
Bond CR, Knight EC, Walker TK (1937) The production of glucosone from carbohydrates by enzymic action. Biochem J 31:1033–1040
Eriksson K-E, Johnsrud SC (1983) Mutants of the white-rot fungus Sporotrichum pulverulentum with increased cellulase and β-d-glucosidase production. Enzyme Microb Technol 5:425–429
Eriksson K-E, Johnsrud SC, Vallander L (1983) Degradation of lignin and lignin model compounds by various mutants of the white-rot fungus Sporotrichum pulverulentum. Arch Microbiol 135:161–168
Faison B, Kirk TK (1983) Relationship between lignin degradation and production of reduced oxygen species by Phanerochaete chrysosporium. Appl Environ Microbiol 46:1140–1145
Forney LJ, Reddy CA, Tien M, Aust SD (1982) The involvement of hydroxyl radical derived from hydrogen peroxide in lignin degradation by the white-rot fungus Phanerochaete chrysosporium. J Biol Chem 257:11455–11462
Forney LJ, Reddy CA, Pankratz HS (1982a) Ultrastructural localization of hydrogen peroxide production in ligninolytic Phanerochaete chrysosporium cells. Appl Env Microbiol 44:732–736
Geigert J, Neidleman SL, Hirano DS, Wolf B, Panschar BM (1983) Enzymic oxidation of di-arabino-hexose-2-ulose[d-glucozone] to d-arabino-2-hexulosonic acid[2-keto-d-gluconic acid]. Carbohyd Res 113:163–165
Glenn JK, Morgan MA, Mayfield MB, Kuwahara M, Gold MH (1983) An extracellular H2O2-requiring enzyme preparation involved in lignin biodegradation by the white-rot basidiomycete Phanerochaete chrysosporium. Biochem Biophys Res Commun 114:1077–1083
Henseke G, Libenow W (1954) Über Ozonhydrazone. III. Mitteilung über die Bedeutung der asymmetrisch disubstituierten Hydrazine als Reagenz auf Ozon. Chem Ber 87:1068–1077
Henseke G, Winter M (1959) Ozone hydrazones XII. Ozone hydrazones and their conversion to ozone formazans and osazone formazans. Ber Deut Chem Ges 92:3156–3166
Holbrook IB, Leaver AG (1976) A procedure to increase the sensitivity of staining by Coomassie brilliant blue G250 — perchloric acid solution. Anal Biochem 75:634–636
Janssen FW, Ruelius HW (1968) Carbohydrate oxidase, a novel enzyme from Polyporus obtusus. II. Specificity and characterization of reaction products. Biochim Biophys Acta 167:501–510
Jeffries TW, Choi S, Kirk TK (1985) Nutritional regulation of lignin degradation by Phanerochaete chrysosporium. Appl Env Microbiol 42:290–296
Johnsrud SC, Eriksson K-E (1985) Cross-beeding of selected and mutated homokaryotic strains of Phanerochaete chrysosporium K-3: New cellulase deficient strains with increased ability to degrade lignin. Appl Microbiol Biotechnol 21:320–327
Kirk TK, Fenn P (1982) Formation and action of the ligninolytic system in basidiomycetes. In: Frankland, Hedger, Swift (eds) Decomposer Basidiomycetes. Cambridge University Press, pp 67–90
Kocourek J, Ticha M, Kostir J, Jensovsky L (1966) The use of diphenylamine-aniline-phosphoric acid reagent in the detection and differentiation of monosaccharides and their derivatives on paper chromatogram. J Chromatogr 14:228–231
Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 227:680–685
Lloyd JB, Whelan WJ (1969) Enzymatic determination of glucose in the presence of maltose. Anal Biochem 30:467–470
Machida Y, Nakanishi T (1984) Purification and properties of pyranose oxidase from Coriolus versicolor. Agric Biol Chem 48:2463–2470
Neidleman SL, Amon WF, Geigert J (1981) Process for the production of fructose. US Patent 4,246,347
Reddy CA, Forney LJ, Kelly RL (1983) Involvement of hydrogen peroxide-derived hydroxyl radical in lignin degradation by the white-rot fungus Phanerochaete chrysosporium. In: Recent Advances in Lignin Biodegradation Research, Uni Publ Co Ltd, Tokyo, pp 153–163
Reid MS, Bielski RL (1968) A simple apparatus for vertical flatsheet polyacrylamide gel electrophoresis. Anal Biochem 22:374–378
Ruelius HW, Kerwin RM, Janssen FW (1968) Carbohydrate oxidase, a novel enzyme from Polyporus obtusus. I. Isolation and purification. Biochim Biophys Acta 167:493–500
Tien M, Kirk TK (1983) Lignin-degrading enzyme from the hymenomycete Phanerochaete chrysosporium Burds. Science 221:661–663
Vesterberg O (1972) Isoelectric focusing of proteins in polyacrylamide gels. Biochim Biophys Acta 357:11–19
Volc J, Sedmera P, Musilek V (1978) Glucose-2-oxidase activity and accumulation of d-arabino-2-hexosulose in cultures of the basidiomycete Oudemansiella mucida. Folia Microbiol 23:292–298
Volc J, Sedmera P, Musilek V (1979) Conversion of monosaccharides into their corresponding 2-glycosuloses by intact cells of the basidiomycete Oudemansiella mucida. Collection Czechoslov Chem Commun 45:950–955
Volc J, Denisova NP, Nerud F, Musilek V (1985) Glucose-2-oxidase activity in mycelial cultures of basidiomycetes. Folia Microbiol, in press
Weetall HH, Hersh LS (1970) Preparation and characterization of glucose oxidase covalently linked to nickel oxide. Biochim Biophys Acta 206:54–60
Weiss JB, Smith I (1967) Sensitive location reagent for the simultaneous multaneous detection of sugars, amino sugars, and sialic acids. Nature (London) 215:638
White JW Jr, Subers MH, Schepartz AI (1963) The identification of inhibine, the antibacterial factor in honey, as hydrogen peroxide and its origin in a honey glucose-oxidase system. Biochim Biophys Acta 73:57–70
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Eriksson, KE., Pettersson, B., Volc, J. et al. Formation and partial characterization of glucose-2-oxidase, a H2O2 producing enzyme in Phanerochaete chrysosporium . Appl Microbiol Biotechnol 23, 257–262 (1986). https://doi.org/10.1007/BF00261925
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DOI: https://doi.org/10.1007/BF00261925