Summary
Auxotrophic mutant cells of Corynebacterium glycinophilum with high l-serine production activity were immobilized by entrapment with various gel materials, such as synthetic prepolymers and natural polysaccharides. The entrapped cells were used for estimation of l-serine productivity in a medium supplemented with glycine as a precursor. Based on the above criteria, including cell growth in gels and cell leakage from gels, calcium alginate was the most suitable gel material. Continuous l-serine fermentation with calcium alginate-entrapped growing cells was successfully achieved in an air-bubbled reactor for at least 13 days.
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References
Fukui S, Tanaka A (1982) Immobilized microbial cells. Ann Rev Microbiol 36:145–172
Hamilton BK, Hsiao HY, Swann WE, Anderson DM, Delente JJ (1985) Manufacture of l-amino acids with bioreactors. Trends Biotechnol 3:64–68
Ichimura K (1984) A convenient photochemical method to immobilize enzymes. J Polym Sci Chem Ed 22:2817–2828
Kubota K (1985) Improved production of l-serine by mutants of Corynebacterium glycinophilum with less serine dehydratase activity. Agric Biol Chem 49:7–12
Kubota K, Nakazawa H (1985) Carbon-13-NMR spectroscopic studies on l-serine synthesis from glycine in Corynebacterium glycinophilum. Agric Biol Chem 49:2773–2774
Nakajima H, Sonomoto K, Morikawa H, Sato F, Ichimura K, Yamada Y, Tanaka A (1986) Entrapment of Lavandula vera cells with synthetic resin prepolymers and its application to pigment production. Appl Microbiol Biotechnol 24:266–270
Ogaki M, Sonomoto K, Nakajima H, Tanaka A (1986) Continuous production of oxytetracycline by immobilized growing Streptomyces rimosus cells. Appl Microbiol Biotechnol 24:6–11
Okada T, Sonomoto K, Tanaka A (1987a) Application of entrapped growing yeast cells to peptide secretion system. Appl Microbiol Biotechnol 26:112–116
Okada T, Sonomoto K, Tanaka A (1987b) Novel Leu-Lys-specific peptidase (Leulysin) produced by gel-entrapped yeast cells. Biochem Biophys Res Commun 145:316–322
Sirirote P, Yamane T, Shimizu S (1988) l-Serine production from methanol and glycine with an immobilized methyltroph. J Ferment Technol 66:291–297
Slowinski W, Charm SE (1973) Glutamic acid production with gel-entrapped Corynebacterium glutamicum. Biotechnol Bioeng 15:973–979
Sonomoto K, Tanaka A, Omata T, Yamane T, Fukui S (1979) Application of photo-crosslinkable resin prepolymers to entrap microbial cells. Effects of increased cell-entrapping gel hydrophobicity on the hydrocortisone Δ1-dehydrogenation. Eur J Appl Microbiol Biotechnol 6:325–334
Sonomoto K, Jin IN, Tanaka A, Fukui S (1980) Application of urethane prepolymers to immobilization of biocatalysts: Δ1-dehydrogenation of hydrocortisone by Arthrobacter simplex cells entrapped with urethane prepolymers. Agric Biol Chem 44:1119–1126
Sonomoto K, Hoq MM, Tanaka A, Fukui S (1981) Growth of Curvularia lunata spores into mycelial form within various gels, and steroid 11β-hydroxylation by entrapped mycelia. J Ferment Technol 59:465–469
Takashima Y, Nakajima H, Sonomoto K, Tanaka A (1987) Production of daunorubicin by immobilized growing Streptomyces peucetius cells. Appl Microbiol Biotechnol 27:106–109
Tanaka A, Yasuhara S, Osumi M, Fukui S (1977) Immobilization of yeast microbodies by inclusion with photo-crosslinkable resins. Eur J Biochem 80:193–197
Watanabe M, Morinaga Y, Enei H (1987) l-Serine production by a methionine-auxotrophic mutant of methylotrophic Pseudomonas. J Ferment Technol 65:617–620
Yamada H, Miyazaki SS, Izumi Y (1986) l-Serine production by glycine-resistant mutant of methylotrophic Hyphomicrobium methylovorum. Agric Biol Chem 50:17–21
Yongsmith B, Sonomoto K, Tanaka A, Fukui S (1982) Production of vitamin B12 by immobilized cells of a propionic acid bacterium. Eur J Appl Microbiol Biotechnol 16:70–74
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Tanaka, T., Yamamoto, K., Towprayoon, S. et al. Continuous production of l-serine by immobilized growing Corynebacterium glycinophilum cells. Appl Microbiol Biotechnol 30, 564–568 (1989). https://doi.org/10.1007/BF00255360
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DOI: https://doi.org/10.1007/BF00255360