Summary
Actinomycetes were isolated from marine sediments collected in Sandy Hook Bay, New Jersey. A variety of pretreatments, including heat and phenol, were employed to improve the recovery of these microorganisms. In addition, plating of sediment samples on chitin agar, and filtration through cellulose membrane filters were also utilized. These pretreatments eliminated or severely curtailed the growth of contaminating microorganisms thereby facilitating the isolation of actinomycetes. A total of 120 isolates was obtained, of which 19 displayed significant antimicrobial activity. Most of the activity was directed against gram-positive bacteria, but inhibition of gram-negative species and a yeast were also evident.
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Pisano, M.A., Sommer, M.J. & Lopez, M.M. Application of pretreatments for the isolation of bioactive actinomycetes from marine sediments. Appl Microbiol Biotechnol 25, 285–288 (1986). https://doi.org/10.1007/BF00253664
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DOI: https://doi.org/10.1007/BF00253664