Abstract
The Actinobacteria comprise a well-defined clade of bacteria with a high GC content. They are widespread in various ranges of niches. They are present in a diversity of habitats which includes rhizosphere, soil, marine, and freshwater systems. Several Actinobacteria are human and animal pathogens and few of them are pathogenic to plants. Few genera such as Streptomyces and Micromonospora are known for their prolific production of different types of bioactive metabolites, including enzymes, enzyme inhibitors, antibiotics, signaling molecules, and immunomodulators. The present protocol involves collection of Actinobacteria from various freshwater sources like pond and lakes followed by their pretreatment to retard the growth of slime-forming bacteria. The pretreated sample is further diluted and inoculated into a suitable medium followed by enumeration of Actinobacteria. The isolated colonies of actinobacteria are further characterized following Bergey’s Manual of bacteriology. The strains are identified by 16S r-RNA sequencing. The actinobacteria isolated are further tested for production of bioactive metabolites and further antibacterial and antifungal activity of those metabolites is determined.
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Chakraborty, T., Roy, S., Mandal, D., Begum, J., Sengupta, A. (2022). Isolation of Actinobacteria from Water Sources. In: Dharumadurai, D. (eds) Methods in Actinobacteriology. Springer Protocols Handbooks. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1728-1_2
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DOI: https://doi.org/10.1007/978-1-0716-1728-1_2
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-1727-4
Online ISBN: 978-1-0716-1728-1
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