Summary
Embryogenic calli were derived from cultured segments of immature inflorescences of Pennisetum americanum (pearl millet). The original explants as well as the embryogenic calli and the plants regenerated via somatic embryogenesis were examined cytogenetically. Embryogenic calli were predominantly diploid (2n=14) after one month and six months in culture (92% and 76%, respectively). Tetraploid and aneuploid cells were observed in the original explant (2.5% and 1.2%) as well as in one (4.0% and 4.0%) and six-month-old calli (10.0% and 14.0%). Plants were regenerated from calli that had been in continuous culture for two, four and six months. Of the 101 regenerants, 100 were diploid and 1 was tetraploid. The tetraploid was an albino as were three of the diploid regenerants. Examination of 30 of the regenerants in meiotic diakinesis, anaphase I, anaphase II and quartet stages revealed no cytogenetic differences between control and regenerated plants. Gel electrophoresis for total protein content and alcohol dehydrogenase and malate dehydrogenase activity also did not reveal any differences between the controls and regenerants. The results of this study show that a slight shift toward aneuploidy and polyploidy may occur in embryogenic cultures, but there also is a strong selection in favor of plant regeneration from cytogenetically normal cells.
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References
Ahloowahlia BS (1976) Chromosomal changes in parasexually produced ryegrass. In: Jones K, Brandham PE (eds) Current chromosome research. Elsevier/North Holland, Amsterdam, pp 115–122
Bayliss MW (1980) Chromosomal variation in plant tissues in culture. In: Vasil IK (ed) Perspectives in plant cell and tissue culture, suppl 11A. Academic Press, New York, pp 113–144
Bennici A, D'Amato F (1978) In vitro regeneration of Durum wheat plants. 1. Chromosome numbers of regenerated plantlets. Z Pflanzenzücht 81:305–311
Boyes CJ, Vasil IK (1984) Plant regeneration by somatic embryogenesis from cultured young inflorescence segments of Sorghum arundinaceum (Desv.) Stapf, var. ‘sudanense’. Plant Sci Lett 35:153–157
Chen CH, Lo PF, Ross JG (1981) Cytological uniformity in callus culture-derived big bluestem plants (Andropogon gerardii Vitman). Proc S D Acad Sci 60:39–43
Gosch-Wackerle G, Avivi L, Galun E (1979) Induction, culture and differentiation of callus from immature rachises, seeds and embryos of Triticum. Z Pflanzenphysiol 91:267–278
Hanna WW, Lu CY, Vasil IK (1984) Uniformity of plants regenerated from somatic embryos of Panicum maximum Jacq. (guinea grass). Theor Appl Genet 67:155–159
Heinz DJ, Mee GWP (1971) Morphologic, cytogenetic, and enzymatic variation in Saccharum species hybrid clones derived from callus tissue. Am J Bot 58:257–262
Heyser JW, Nabors MW (1982) Regeneration of Proso millet from embryogenic calli derived from various plant parts. Crop Sci 22:1070–1074
Karp A, Maddock SE (1984) Chromosome variation in wheat plants regenerated from cultured immature embryos. Theor Appl Genet 67:249–255
Laemmli V (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680–685
Larkin PJ, Scowcroft WR (1981) Somaclonal variation — a novel source of variability from cell cultures for plant improvement. Theor Appl Genet 60:197–214
Lu CY, Vasil IK (1982) Somatic embryogenesis and plant regeneration in tissue cultures of Panicum maximum Jacq. Am J Bot 69:77–81
McCoy TJ, Phillips RL (1982) Chromosome stability in maize (Zea mays) tissue cultures and sectoring in some regenerated plants. Can J Genet Cytol 24:559–565
McCoy TJ, Phillips RL, Rines HW (1982) Cytogenetic analysis of plants regenerated from oat (Avena sativa) tissue cultures; high frequency of partial chromosome loss. Can J Genet Cytol 24:37–50
Morrissey JH (1981) Silver stain for proteins in polyacrylamide gels: a modified procedure with enhanced uniform sensitivity. Anal Biochem 117:307–310
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497
Orton TJ (1980) Chromosomal variability in tissue cultures and regenerated plants of Hordeum. Theor Appl Genet 56:101–112
Ozias-Akins P, Vasil IK (1982) Plant regeneration from cultured immature embryos and inflorescences of Triticum aestivum (wheat): evidence for somatic embryogenesis. Protoplasma 110:95–105
Schwartz D, Endo T (1966) Alcohol dehydrogenase polymorphism in maize-simple and compound loci. Genetics 53: 709–715
Vasil IK (1982) Somatic embryogenesis and plant regeneration in cereals and grasses. In: Fujiwara A (ed) Plant tissue culture. Maruzen, Tokyo, pp 101–104
Vasil IK (1983a) Regeneration of plants from single cells of cereals and grasses. In: Lurquin P, Kleinhofs A (eds) Genetic engineering in eukaryotes. Plenum, New York, pp 233–252
Vasil IK (1983b) Toward the development of a single cell system for grasses. In: Cell and tissue culture techniques for cereal crop improvement. Science Press, Peking, pp 131–144
Vasil V, Vasil IK (1981a) Somatic embryogenesis and plant regeneration from inflorescence segments of Pennisetum americanum and P. americanum x P. purpureum hybrids. Am J Bot 68:864–872
Vasil V, Vasil IK (1981b) Somatic embryogenesis and plant regeneration from suspension cultures of pearl millet (Pennisetum americanum). Ann Bot 47:669–678
Wang DY, Vasil IK (1982) Somatic embryogenesis and plant regeneration from inflorescence segments of Pennisetum purpureum Schum. (Napier or elephant grass). Plant Sci Lett 25:147–154
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Communicated by P. L. Pfahler
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Swedlund, B., Vasil, I.K. Cytogenetic characterization of embryogenic callus and regenerated plants of Pennisetum americanum (L.) K. Schum. Theoret. Appl. Genetics 69, 575–581 (1985). https://doi.org/10.1007/BF00251107
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DOI: https://doi.org/10.1007/BF00251107