Summary
A cryopreservation process using encapsulation/dehydration was set up for apices sampled on in vitro plantlets of sugarcane. After dissection, apices were cultured for one day on standard medium and then encapsulated in medium with 3% alginate. Optimal conditions comprised preculture for 2 days in liquid medium with 250 g.l−1 sucrose, desiccation for 6 hours under the laminar flow or for 10–11 hours with silicagel followed by rapid freezing and slow thawing. Survival after freezing in liquid nitrogen ranged between 38 and 91% for the 5 varieties experimented. Cryopreservation did not modify the electrophoretic profiles for aminoleucine peptidases and amylases with plants of the variety Co 6415.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
Abbreviations
- BAP:
-
6-benzylaminopurine
- KIN:
-
Kinetin
- EDTA:
-
ethylenediamine tetracetic acid
- AMP:
-
aminoleucine peptidases
- AMY:
-
amylases
- RFLP:
-
restriction fragment length polymorphism
References
Bajaj YPS, Komeva SB, Gutierrez R, Maribona RH (1987) In Proc. Int. Conf. on Cryogenics. A. Bose and P. Sengupta eds., McGraw-Hill, New Delhi, pp 222–226
Benson EE, Noronha-Dutra AA (1988) Cryo-Letters 9:120–131
Benson EE, Harding K, Smith H (1989) Cryo-Letters 10:323–344
Benson EE, Chabrillange TT, Engelmann F (1992) In Proc. Society for Low Temperature Biology Autumn Meeting, Stirling, UK, 8–9/09/92, in press
Berding N, Roach BT (1987) In Developments in Crop Science, Vol. 11, Sugarcane Improvement through Breeding. DJ Heinz ed., Elsevier, Amsterdam, pp 143–210
Chagvardieff P (1980) Thèse de Docteur Ingénieur, Amélioration des Plantes, Université Paris Sud Orsay
Crowe JH, Crowe LM, Carpenter JF, Rudolph AS, Wistrom CA, Spargo BJ, Anchordoguy TJ (1988) Biochim. Biophys. Acta 947:367–384
Dereuddre J, Engelmann F (1987) In Proc. Coll. Franco-Britannique IAPTC, Angers, 8–9 oct.1987, pp 48–78
Dereuddre J (1992) In Reproductive Biology and Plant Breeding. Y. Dattée, C. Dumas and A. Gallais eds., Springer Verlag, Berlin, pp 291–300
Dereuddre J, Scottez C, Arnaud Y, Duron M (1990) CR. Acad. Sci. Paris 310 (III):317–323
Dumet D, Engelmann F, Chabrillange N, Duval Y (1993) Plant Cell Rep. in press
Dussert S, Mauro MC, Engelmann F (1992) Cryo-Letters 13:12–22
Eksomtramage T (1993) Thèse d'Université, Rennes 1
Eksomtramage T, Paulet F, Guiderdoni E, Glaszmann JC, Engelmann F (1992a) Cryo-Letters 13:239–252
Eksomtramage T, Paulet F, Noyer JL, Feldmann P, Glaszmann JC (1992b) Sugarcane 3:14–21
Engelmann F (1991a) Euphytica 57:227–243
Engelmann F (1991b) In Proc. XVIIIè Cong. Int. Froid, Montréal, 10–17 août, vol. IV, pp 1676–1680
Feldmann P (1984) Thèse de 3èmecycle, Développement et Amélioration des Végétaux, Université Paris XI
Fuji T (1970) Wheat Inform Serv. 31:1–2
Glaszmann JC, Lu YH, D'Hont A, Feldmann P, Gonzalez de Leon D, Lanaud C (in press) In Proc. 21st Int. Soc. Sugarcane Technol. Cong., Bangkok, Thailand, march 1992
Gnanapragasam S, Vasil IK (1990) Plant Cell Rep. 9:419–423
Kartha KK (1985) In Cryopreservation of Plant Cells and Organs. KK Kartha ed., CRC Press, Boca Raton Florida, pp 115–134
Kendall EJ, Qureshi JA, Kartha KK, Leung N, Chewier N, Caswell K, Chen THH (1990) Plant Physiol. 94:1756–1762
Ling CJ, De LS, Long HS (1987) In Plant Biology, Vol.5, Plant Cold Hardiness. PH Li ed., Alan R. Liss Inc., New York, pp 323–337
Murashige T, Skoog F (1962) Physiol. Plant. 15:473–497
Paulet F, Acquaviva C, Eksomtramage T, Lu YH, D'Hont A, Glaszmann JC (1991) In Actes de la première rencontre internationale française sur la canne à sucre. AFCAS, Nogent-Sur-Marne, France, pp 47–52
Peros JP, Bonnel E, Féréol L, Mauboussin JC (1989) In Proc. 20th Int. Soc. Sugarcane Technol. Cong., Sao Paulo, Brazil, pp 732–739
Plessis P, Leddet C, Dereuddre J (1991) C.R. Acad. Sci. Paris 313 (III):373–380
Skinner JC, Hogarth DM, Wu KK (1987) In Developments in Crop Science, Vol. 11, Sugarcane Improvement through Breeding. DJ Heinz ed., Elsevier, Amsterdam, pp 409–453
Ulrich JM, Finkle BJ, Moore PH (1984) Sugarcane 3:11–14
Uragami A (1990) PhD Thesis, Hokkaido Univ.
Watanabe K, Ueno S, Mitsuda H (1990) Agric. Biol. Chem. 54:1295–1296
Watanabe K, Yamada Y, Ueno S, Mitsuda H (1985) Agric. Biol. Chem. 49:1727–1731
Withers LA (1987) Oxford Survey of Plant Molec. Cell Biol. 4:221–272
Author information
Authors and Affiliations
Additional information
Communicated by A. M. Boudet
Rights and permissions
About this article
Cite this article
Paulet, F., Engelmann, F. & Glaszmann, JC. Cryopreservation of apices of in vitro plantlets of sugarcane (Saccharum sp. hybrids) using encapsulation/dehydration. Plant Cell Reports 12, 525–529 (1993). https://doi.org/10.1007/BF00236101
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00236101