Summary
When cell suspension cultures of Catharanthus roseus are treated with autoclaved elicitor from the fungus Pythium aphanidermatum, they respond with the rapid transient induction of tryptophan decarboxylase (TDC) and strictosidine synthase (SS) enzyme activities, followed by the accumulation of indole alkaloids (Eilert et al., 1987). In this report, we demonstrate that expression of TDC and SS enzyme activities is preceeded by the transient appearance of mRNAs for both enzymes, suggesting transcriptional control of these events. The strong transient accumulation of both TDC and SS enzyme transcripts observed in elicitor-treated cell suspension cultures contrasts with the barely detectable level of TDC transcripts and the undetectable level of SS transcripts observed in developing seedlings.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
Abbreviations
- TDC:
-
tryptophan decarboxylase
- SS:
-
strictosidine synthase
References
Aerts R.J., T. Van Der Leer, R. Van Der Heijden & R. Verpoorte (1990) J. Plant Physiol. 136:86–91.
Aerts R.J., A. de Waal, E.J.M. Pennings & R. Verpoorte (1991) Planta 183:536–541.
Aviv H. & P. Leder (1972) Proc. Nat. Acad. Sci. USA 69:1408–1412.
Balsevich J., V. De Luca & W.G.W. Kurz (1986) Heterocycles 24:2415–2421.
De Luca V., J. Balsevich, B. Tyler, U. Eilert, B. Panchuk & W.G.W. Kurz (1986) J. Plant Physiol. 125:147–156.
De Luca V., J. Alvarez-Fernandez, D. Campbell & W.G.W. Kurz (1988) Plant Physiol. 86:447–450.
De Luca V., C. Marineaux & N. Brisson (1989) Proc. Nat. Acad. Sci. 86:2582–2586.
Eilert U., V. De Luca, F. Constabel & W.G.W. Kurz (1987) Arch. Biochem. Biophys. 254: 491–497.
Fernandez J.A., T.G. Owen, W.G.W. Kurz & V. De Luca (1989a) Plant Physiol. 91:79–84.
Knobloch K.H., B. Hansen, J. Berlin (1981) Z. Naturforsch. 36c:40–43.
Kutchan T.M., N. Hampp, F. Lottspeich, M.H. Zenk (1988) FEBS Lett. 237:127–130.
Jones J.D.G., P. Dunsmuir & J. Bedbrook (1985) EMBO J 4:2411–2418.
Maniatis T., E.F. Fritch & J. Sambrook (1982) In “Molecular Cloning: A Laboratory Manual (Cold Spring Harbor Lab. Cold Spring Harbor NY).
McNight T.D., C.A. Roessner, R. Devagupta, C.L. Nessler (1990) Nucleic Acids Res. 18: 4939.
McNight T.D., D.R. Bergey, R.J. Burnett, & C.L. Nessler (1991) Planta 185:148–152.
Noé W. & J. Berlin (1985) Planta 166: 500–504.
Scott A.I., S. Lee, M.G. Culver, W. Wan, T. Hirata, F. Gueritte, R.L. Baxter, H. Nordlov, C.A. Dorschel, H. Mizukami, N.E. Mackenzie, (1981) Heterocycles 15: 1257–1274.
Stöckigt J. (1980). In “Indole and Biogenetially Related Alkaloids” (J.D. Phillipson and M.H. Zenk, eds) Academic Press, London, pp 113–141.
Author information
Authors and Affiliations
Additional information
Communicated by F. Constabel
Rights and permissions
About this article
Cite this article
Roewer, I.A., Cloutier, N., Nessler, C.L. et al. Transient induction of tryptophan decarboxylase (TDC) and strictosidine synthase (SS) genes in cell suspension cultures of Catharanthus roseus . Plant Cell Reports 11, 86–89 (1992). https://doi.org/10.1007/BF00235259
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00235259