Summary
Production of L-DOPA was studied in cell suspension culture of Mucuna pruriens f. pruriens. Suspension culture was established in MSI medium composed of half concentration of Murashige and Skoog's salts and 2% sucrose. A two-stage cell suspension culture was developed for enhanced accumulation of L-DOPA. In the first stage, the culture system was composed of MSI medium without CaCl, which was suitable for cell growth and in the second stage MSI medium containing 42.5 mg.l−1 KH2PO4 and 4% sucrose favoured L-DOPA production. A discernible higher production of L-DOPA was obtained in this two-stage cell suspension culture in comparison to single stage culture.
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Communicated by A. R. Gould
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Chattopadhyay, S., Datta, S.K. & Mahato, S.B. Production of L-DOPA from cell suspension culture of Mucuna pruriens f. pruriens . Plant Cell Reports 13, 519–522 (1994). https://doi.org/10.1007/BF00232948
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DOI: https://doi.org/10.1007/BF00232948