Abstract
Halved shoot bases of Allium tuberosum Rottl. ex Spreng. proliferated both axillary and adventitious shoots on B5 medium (1968) supplemented with either 6-benzylaminopurine (0.5 mg/l) or 1-naphthalene acetic acid (0.1 mg/l) and 2-isopentenyladenine (0.5 mg/l). In vitro shoots proliferated further numerous shoots upon subculture to fresh medium, and these shoots rooted spontaneously. Plantlets were transplanted successfully to soil and retained the diploid condition of the parents.
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Abbreviations
- B5:
-
Gamborg et al. (1968) medium
- BAP:
-
6-benzylaminopurine
- 2ip:
-
2isopentenyladenine
- NAA:
-
1-naphthalene acetic acid
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Communicated by G. C. Phillips
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Radhamani, J., Chandel, K.P.S. Cryopreservation of embryonic axes of trifoliate orange (Poncirus trifoliata [L.] RAF.). Plant Cell Reports 11, 204–206 (1992). https://doi.org/10.1007/BF00232534
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DOI: https://doi.org/10.1007/BF00232534