Abstract
Protoplasts were isolated from cotyledon, hypocotyl and mesophyll cells of Corchorus capsularis L., a major fibre crop, by one step enzyme digestion method. They were further cultured successfully on modified KM-8p (Kao and Michayluk, 1975) medium to form microcalli. The required cultural conditions could be used to achieve 34% to 78% plating efficiency, depending upon the source of protoplasts. Hypocotyl protoplasts gave the highest plating efficiency. On transfer to regeneration medium, somatic embryos developed at high frequency. The present success is a significant step forward in the development of meaningful plant cell culture methods for application in jute.
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Abbreviations
- B5 :
-
Gamborg et al., 1968
- MS:
-
Murashige and Skoog, 1962
- SH:
-
Schenk and Hildebrandt, 1972
- Ad-SO4 :
-
Adenine sulphate
- BAP:
-
6-benzylaminopurine
- NAA:
-
1-naphthalene acetic acid
- GA3 :
-
Gibberellic acid
- 2,4-D:
-
2,4-dichlorophenoxy acetic acid
- Kn:
-
Kinetin
- IBA:
-
Indole-3-butyric acid
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Communicated by G. C. Phillips
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Saha, T., Sen, S.K. Somatic embryogenesis in protoplast derived calli of cultivated jute, Corchorus capsularis L.. Plant Cell Reports 10, 633–636 (1992). https://doi.org/10.1007/BF00232386
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DOI: https://doi.org/10.1007/BF00232386